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Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization
Zhong, Xue-Ping1,2; Wang, Dan1; Zhang, Yi-Bing1; Gui, Jian-Fang1; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009-02-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume152Issue:2Pages:161-170
AbstractOrganisms living in water are inevitably exposed to periods of hypoxia. Environmental hypoxia has been an important stressor having manifold effects on aquatic life. Many fish species have evolved behavioral, physiological, biochemical and molecular adaptations that enable them to cope with hypoxia. However, the molecular mechanisms of hypoxia tolerance in fish, remain unknown. in this study, we used suppression subtractive hybridization to examine the differential gene expression in CAB cells (Carassius auratus blastulae embryonic cells) exposed to hypoxia for 24 h. We isolated 2100 clones and identified 211 differentially expressed genes (e-value <= 5e-3; Identity > 45%). Among the genes whose expression is modified in cells, a vast majority involved in metabolism, signal transduction, cell defense, angiogenesis, cell growth and proliferation. Twelve genes encoding for ERO1-L, p53, CPO, HO-1, MKP2, PFK-2, cystatin B, GLUT1, BTG1, TGF beta 1, PGAM1, hypothetical protein F1508, were selected and identified to be hypoxia-induced using semi-quantitive RT-PCR and real-time PCR. Among the identified genes, two open reading frames (ORFs) encoding for CaBTG1 and Cacystatin B were obtained. The deduced amino acid sequence of CaBTG1 had 94.1%, 72.8%, 72.8%, 72.8%, 68.6% identity with that of DrBTG1, HsBTG1, BtBTG1, MmBTG1 and XIBTG1. Comparison of Cacystatin B with known cystatin B, the molecules exhibited 49.5 to 76.0% identity overall. These results may provide significant information for further understanding of the adaptive mechanism by which C. auratus responds to hypoxia. (c) 2008 Elsevier Inc. All rights reserved.; Organisms living in water are inevitably exposed to periods of hypoxia. Environmental hypoxia has been an important stressor having manifold effects on aquatic life. Many fish species have evolved behavioral, physiological, biochemical and molecular adaptations that enable them to cope with hypoxia. However, the molecular mechanisms of hypoxia tolerance in fish, remain unknown. in this study, we used suppression subtractive hybridization to examine the differential gene expression in CAB cells (Carassius auratus blastulae embryonic cells) exposed to hypoxia for 24 h. We isolated 2100 clones and identified 211 differentially expressed genes (e-value <= 5e-3; Identity > 45%). Among the genes whose expression is modified in cells, a vast majority involved in metabolism, signal transduction, cell defense, angiogenesis, cell growth and proliferation. Twelve genes encoding for ERO1-L, p53, CPO, HO-1, MKP2, PFK-2, cystatin B, GLUT1, BTG1, TGF beta 1, PGAM1, hypothetical protein F1508, were selected and identified to be hypoxia-induced using semi-quantitive RT-PCR and real-time PCR. Among the identified genes, two open reading frames (ORFs) encoding for CaBTG1 and Cacystatin B were obtained. The deduced amino acid sequence of CaBTG1 had 94.1%, 72.8%, 72.8%, 72.8%, 68.6% identity with that of DrBTG1, HsBTG1, BtBTG1, MmBTG1 and XIBTG1. Comparison of Cacystatin B with known cystatin B, the molecules exhibited 49.5 to 76.0% identity overall. These results may provide significant information for further understanding of the adaptive mechanism by which C. auratus responds to hypoxia. (c) 2008 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordCarassius Auratus Hypoxia Suppression Subtractive Hybridization
Department[Zhong, Xue-Ping; Wang, Dan; Zhang, Yi-Bing; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhong, Xue-Ping] Cent China Normal Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Zoology
DOI10.1016/j.cbpb.2008.10.013
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30500375]; 973 National Basic Research Program of China [2004CB117404]; 863 High Technology Research Program of China [2007AA09Z423] ; National Natural Science Foundation of China [30500375]; 973 National Basic Research Program of China [2004CB117404]; 863 High Technology Research Program of China [2007AA09Z423] ; National Natural Science Foundation of China [30500375]; 973 National Basic Research Program of China [2004CB117404]; 863 High Technology Research Program of China [2007AA09Z423] ; National Natural Science Foundation of China [30500375]; 973 National Basic Research Program of China [2004CB117404]; 863 High Technology Research Program of China [2007AA09Z423]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS IDWOS:000263140700008
WOS KeywordENDOTHELIAL GROWTH-FACTOR ; MICROSOMAL HEME OXYGENASE ; ANOXIC CRUCIAN CARP ; MESSENGER-RNA ; INDUCIBLE FACTOR-1 ; OXIDATIVE STRESS ; ANAEROBIC METABOLISM ; EXPRESSION ANALYSIS ; CATHEPSIN-D ; GRASS CARP
Funding OrganizationNational Natural Science Foundation of China [30500375]; 973 National Basic Research Program of China [2004CB117404]; 863 High Technology Research Program of China [2007AA09Z423] ; National Natural Science Foundation of China [30500375]; 973 National Basic Research Program of China [2004CB117404]; 863 High Technology Research Program of China [2007AA09Z423] ; National Natural Science Foundation of China [30500375]; 973 National Basic Research Program of China [2004CB117404]; 863 High Technology Research Program of China [2007AA09Z423] ; National Natural Science Foundation of China [30500375]; 973 National Basic Research Program of China [2004CB117404]; 863 High Technology Research Program of China [2007AA09Z423]
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7884
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Cent China Normal Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Xue-Ping,Wang, Dan,Zhang, Yi-Bing,et al. Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2009,152(2):161-170.
APA Zhong, Xue-Ping,Wang, Dan,Zhang, Yi-Bing,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,152(2),161-170.
MLA Zhong, Xue-Ping,et al."Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 152.2(2009):161-170.
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