IHB OpenIR  > 期刊论文
Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes
Gao, Hong; Xu, Xudong; Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2009-03-01
Source PublicationFEMS MICROBIOLOGY LETTERS
ISSN0378-1097
Volume292Issue:1Pages:63-70
AbstractA vipp1 mutant of Synechocystis sp. PCC 6803 could not be completely segregated under either mixotrophic or heterotrophic conditions. A vipp1 gene with a copper-regulated promoter (P-petE-vipp1) was integrated into a neutral platform in the genome of the merodiploid mutant. The copper-induced expression of P-petE-vipp1 allowed a complete segregation of the vipp1 mutant and observation of the phenotype of Synechocystis 6803 with different levels of vesicle-inducing protein in plastids 1 (Vipp1). When P-petE-vipp1 was turned off by copper deprivation, Synechocystis lost Vipp1 and photosynthetic activity almost simultaneously, and at a later stage, thylakoid membranes and cell viability. The photosystem II (PSII)-mediated electron transfer was much more rapidly reduced than the PSI-mediated electron transfer. By testing a series of concentrations, we found that P-petE-vipp1 cells grown in medium with 0.025 mu M Cu2+ showed no reduction of thylakoid membranes, but greatly reduced photosynthetic activity and viability. These results suggested that in contrast to a previous report, the loss of photosynthetic activity may not have been due to the loss of thylakoid membranes, but may have been caused more directly by the loss of Vipp1 in Synechocystis 6803.; A vipp1 mutant of Synechocystis sp. PCC 6803 could not be completely segregated under either mixotrophic or heterotrophic conditions. A vipp1 gene with a copper-regulated promoter (P(petE)-vipp1) was integrated into a neutral platform in the genome of the merodiploid mutant. The copper-induced expression of P(petE)-vipp1 allowed a complete segregation of the vipp1 mutant and observation of the phenotype of Synechocystis 6803 with different levels of vesicle-inducing protein in plastids 1 (Vipp1). When P(petE)-vipp1 was turned off by copper deprivation, Synechocystis lost Vipp1 and photosynthetic activity almost simultaneously, and at a later stage, thylakoid membranes and cell viability. The photosystem II (PSII)-mediated electron transfer was much more rapidly reduced than the PSI-mediated electron transfer. By testing a series of concentrations, we found that P(petE)-vipp1 cells grown in medium with 0.025 mu M Cu(2+) showed no reduction of thylakoid membranes, but greatly reduced photosynthetic activity and viability. These results suggested that in contrast to a previous report, the loss of photosynthetic activity may not have been due to the loss of thylakoid membranes, but may have been caused more directly by the loss of Vipp1 in Synechocystis 6803.
SubtypeArticle
KeywordVipp1 p(Pete) Photosynthesis Thylakoid Membranes Viability Synechocystis
Department[Gao, Hong; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Subject AreaMicrobiology
DOI10.1111/j.1574-6968.2008.01470.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332] ; National Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332] ; National Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332] ; National Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000263036900010
WOS KeywordSP STRAIN PCC-6803 ; CHILL-LIGHT TOLERANCE ; PHOTOSYSTEM-II ; MOLECULAR-CLONING ; GENE ; BIOGENESIS ; EXPRESSION ; PLASMA ; GROWTH ; MUTANT
Funding OrganizationNational Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332] ; National Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332] ; National Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332] ; National Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332]
Citation statistics
Cited Times:53[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7876
Collection期刊论文
Corresponding AuthorXu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Gao, Hong,Xu, Xudong,Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China. Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes[J]. FEMS MICROBIOLOGY LETTERS,2009,292(1):63-70.
APA Gao, Hong,Xu, Xudong,&Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.(2009).Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes.FEMS MICROBIOLOGY LETTERS,292(1),63-70.
MLA Gao, Hong,et al."Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes".FEMS MICROBIOLOGY LETTERS 292.1(2009):63-70.
Files in This Item:
File Name/Size DocType Version Access License
Depletion of Vipp1 i(67KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Gao, Hong]'s Articles
[Xu, Xudong]'s Articles
[Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China]'s Articles
Baidu academic
Similar articles in Baidu academic
[Gao, Hong]'s Articles
[Xu, Xudong]'s Articles
[Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Gao, Hong]'s Articles
[Xu, Xudong]'s Articles
[Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.