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学科主题: Microbiology
题名: Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes
作者: Gao, Hong1; Xu, Xudong1
通讯作者: Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
关键词: Vipp1 ; P(petE) ; photosynthesis ; thylakoid membranes ; viability ; Synechocystis
刊名: FEMS MICROBIOLOGY LETTERS
发表日期: 2009-03-01
DOI: 10.1111/j.1574-6968.2008.01470.x
卷: 292, 期:1, 页:63-70
收录类别: SCI
文章类型: Article
部门归属: [Gao, Hong; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332]
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: A vipp1 mutant of Synechocystis sp. PCC 6803 could not be completely segregated under either mixotrophic or heterotrophic conditions. A vipp1 gene with a copper-regulated promoter (P-petE-vipp1) was integrated into a neutral platform in the genome of the merodiploid mutant. The copper-induced expression of P-petE-vipp1 allowed a complete segregation of the vipp1 mutant and observation of the phenotype of Synechocystis 6803 with different levels of vesicle-inducing protein in plastids 1 (Vipp1). When P-petE-vipp1 was turned off by copper deprivation, Synechocystis lost Vipp1 and photosynthetic activity almost simultaneously, and at a later stage, thylakoid membranes and cell viability. The photosystem II (PSII)-mediated electron transfer was much more rapidly reduced than the PSI-mediated electron transfer. By testing a series of concentrations, we found that P-petE-vipp1 cells grown in medium with 0.025 mu M Cu2+ showed no reduction of thylakoid membranes, but greatly reduced photosynthetic activity and viability. These results suggested that in contrast to a previous report, the loss of photosynthetic activity may not have been due to the loss of thylakoid membranes, but may have been caused more directly by the loss of Vipp1 in Synechocystis 6803.
英文摘要: A vipp1 mutant of Synechocystis sp. PCC 6803 could not be completely segregated under either mixotrophic or heterotrophic conditions. A vipp1 gene with a copper-regulated promoter (P(petE)-vipp1) was integrated into a neutral platform in the genome of the merodiploid mutant. The copper-induced expression of P(petE)-vipp1 allowed a complete segregation of the vipp1 mutant and observation of the phenotype of Synechocystis 6803 with different levels of vesicle-inducing protein in plastids 1 (Vipp1). When P(petE)-vipp1 was turned off by copper deprivation, Synechocystis lost Vipp1 and photosynthetic activity almost simultaneously, and at a later stage, thylakoid membranes and cell viability. The photosystem II (PSII)-mediated electron transfer was much more rapidly reduced than the PSI-mediated electron transfer. By testing a series of concentrations, we found that P(petE)-vipp1 cells grown in medium with 0.025 mu M Cu(2+) showed no reduction of thylakoid membranes, but greatly reduced photosynthetic activity and viability. These results suggested that in contrast to a previous report, the loss of photosynthetic activity may not have been due to the loss of thylakoid membranes, but may have been caused more directly by the loss of Vipp1 in Synechocystis 6803.
关键词[WOS]: SP STRAIN PCC-6803 ; CHILL-LIGHT TOLERANCE ; PHOTOSYSTEM-II ; MOLECULAR-CLONING ; GENE ; BIOGENESIS ; EXPRESSION ; PLASMA ; GROWTH ; MUTANT
语种: 英语
WOS记录号: WOS:000263036900010
ISSN号: 0378-1097
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7876
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Gao, Hong; Xu, Xudong.Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes,FEMS MICROBIOLOGY LETTERS,2009,292(1):63-70
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