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学科主题: Fisheries; Marine & Freshwater Biology
题名: Detection of serum and mucosal antibody production and antibody secreting cells (ASCs) in large yellow croaker (Pseudosciaena crocea) following vaccination with Vibrio harveyi via different routes
作者: Xu, Zhen1, 2, 3; Chen, Chang-Fu1, 2; Mao, Zhi-Juan4; Zhu, Wu-Ying4
通讯作者: Chen, CF, Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China
关键词: Vibrio harveyi ; Pseudosciaena crocea ; Systemic antibodies ; Mucosal antibodies ; Antibody secreting cells
刊名: AQUACULTURE
发表日期: 2009-02-18
DOI: 10.1016/j.aquaculture.2008.10.026
卷: 287, 期:3-4, 页:243-247
收录类别: SCI
文章类型: Article
部门归属: [Xu, Zhen; Chen, Chang-Fu] Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China; [Xu, Zhen; Chen, Chang-Fu] Huazhong Agr Univ, Minist Educ, Key Lab Agr Microbiol, Wuhan 430070, Peoples R China; [Xu, Zhen] Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Mao, Zhi-Juan; Zhu, Wu-Ying] Zhejiang Univ, Coll Biomed Engn & Instrument Sci, Hangzhou 310027, Zhejiang, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30271032]
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
摘要: The kinetics of mucosal and serum antibody response is well as antibody secreting cells (ASCs) production were studied in large yellow croaker following vaccination with inactivated Vibrio harveyi by different routes: oral administration. intraperitoneal (IP) injection and immersion. Indirect ELISA was used to measure the antibody level in serum and cutaneous mucus, and ELISPOT was used to monitor the ASCs derived from gill, blood and head kidney. The data demonstrated that IP injection resulted in the highest antibody levels in the systemic circulation, whereas immersion induced significant antibody levels in mucous. As for the ASCs response, IP injection induced high numbers of ASCs in the head kidney and blood; oral intubation only induced a slight ASCs response in the head kidney: immersion induced a much stronger ASCs response in the gill. These results indicate that mucosal antibodies following immersion immunization are independent of a systemic response and more sensitive, since it could be triggered earlier than serum antibodies. The mucosal antibodies following IP injection immunization may depend oil a systemic immune response. The protective effects of the three vaccination methods were compared by challenging with live V. harveyi. Survival of the three groups of vaccinated fish varied front 40 to 60%. while 100% mortality was found in control fish. Compared with IP and oral vaccination, immersion stimulated higher specific antibody titers in the mucosal system and achieved similar protection, so it is in effective and efficient method for immunizing a large number of fish against V harveyi (C) 2008 Elsevier B.V. All rights reserved.
英文摘要: The kinetics of mucosal and serum antibody response is well as antibody secreting cells (ASCs) production were studied in large yellow croaker following vaccination with inactivated Vibrio harveyi by different routes: oral administration. intraperitoneal (IP) injection and immersion. Indirect ELISA was used to measure the antibody level in serum and cutaneous mucus, and ELISPOT was used to monitor the ASCs derived from gill, blood and head kidney. The data demonstrated that IP injection resulted in the highest antibody levels in the systemic circulation, whereas immersion induced significant antibody levels in mucous. As for the ASCs response, IP injection induced high numbers of ASCs in the head kidney and blood; oral intubation only induced a slight ASCs response in the head kidney: immersion induced a much stronger ASCs response in the gill. These results indicate that mucosal antibodies following immersion immunization are independent of a systemic response and more sensitive, since it could be triggered earlier than serum antibodies. The mucosal antibodies following IP injection immunization may depend oil a systemic immune response. The protective effects of the three vaccination methods were compared by challenging with live V. harveyi. Survival of the three groups of vaccinated fish varied front 40 to 60%. while 100% mortality was found in control fish. Compared with IP and oral vaccination, immersion stimulated higher specific antibody titers in the mucosal system and achieved similar protection, so it is in effective and efficient method for immunizing a large number of fish against V harveyi (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: EDWARDSIELLA-TARDA INFECTION ; BASS DICENTRARCHUS-LABRAX ; TROUT ONCORHYNCHUS-MYKISS ; VULNIFICUS ; IMMERSION ; L. ; PISCICIDA ; EXPOSURE ; KINETICS ; EFFICACY
语种: 英语
WOS记录号: WOS:000263388500002
ISSN号: 0044-8486
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7868
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Minist Educ, Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
4.Zhejiang Univ, Coll Biomed Engn & Instrument Sci, Hangzhou 310027, Zhejiang, Peoples R China

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Xu, Zhen; Chen, Chang-Fu; Mao, Zhi-Juan; Zhu, Wu-Ying.Detection of serum and mucosal antibody production and antibody secreting cells (ASCs) in large yellow croaker (Pseudosciaena crocea) following vaccination with Vibrio harveyi via different routes,AQUACULTURE,2009,287(3-4):243-247
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