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Detection of serum and mucosal antibody production and antibody secreting cells (ASCs) in large yellow croaker (Pseudosciaena crocea) following vaccination with Vibrio harveyi via different routes
Xu, Zhen1,2,3; Chen, Chang-Fu1,2; Mao, Zhi-Juan4; Zhu, Wu-Ying4; Chen, CF, Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China
2009-02-18
Source PublicationAQUACULTURE
ISSN0044-8486
Volume287Issue:3-4Pages:243-247
AbstractThe kinetics of mucosal and serum antibody response is well as antibody secreting cells (ASCs) production were studied in large yellow croaker following vaccination with inactivated Vibrio harveyi by different routes: oral administration. intraperitoneal (IP) injection and immersion. Indirect ELISA was used to measure the antibody level in serum and cutaneous mucus, and ELISPOT was used to monitor the ASCs derived from gill, blood and head kidney. The data demonstrated that IP injection resulted in the highest antibody levels in the systemic circulation, whereas immersion induced significant antibody levels in mucous. As for the ASCs response, IP injection induced high numbers of ASCs in the head kidney and blood; oral intubation only induced a slight ASCs response in the head kidney: immersion induced a much stronger ASCs response in the gill. These results indicate that mucosal antibodies following immersion immunization are independent of a systemic response and more sensitive, since it could be triggered earlier than serum antibodies. The mucosal antibodies following IP injection immunization may depend oil a systemic immune response. The protective effects of the three vaccination methods were compared by challenging with live V. harveyi. Survival of the three groups of vaccinated fish varied front 40 to 60%. while 100% mortality was found in control fish. Compared with IP and oral vaccination, immersion stimulated higher specific antibody titers in the mucosal system and achieved similar protection, so it is in effective and efficient method for immunizing a large number of fish against V harveyi (C) 2008 Elsevier B.V. All rights reserved.; The kinetics of mucosal and serum antibody response is well as antibody secreting cells (ASCs) production were studied in large yellow croaker following vaccination with inactivated Vibrio harveyi by different routes: oral administration. intraperitoneal (IP) injection and immersion. Indirect ELISA was used to measure the antibody level in serum and cutaneous mucus, and ELISPOT was used to monitor the ASCs derived from gill, blood and head kidney. The data demonstrated that IP injection resulted in the highest antibody levels in the systemic circulation, whereas immersion induced significant antibody levels in mucous. As for the ASCs response, IP injection induced high numbers of ASCs in the head kidney and blood; oral intubation only induced a slight ASCs response in the head kidney: immersion induced a much stronger ASCs response in the gill. These results indicate that mucosal antibodies following immersion immunization are independent of a systemic response and more sensitive, since it could be triggered earlier than serum antibodies. The mucosal antibodies following IP injection immunization may depend oil a systemic immune response. The protective effects of the three vaccination methods were compared by challenging with live V. harveyi. Survival of the three groups of vaccinated fish varied front 40 to 60%. while 100% mortality was found in control fish. Compared with IP and oral vaccination, immersion stimulated higher specific antibody titers in the mucosal system and achieved similar protection, so it is in effective and efficient method for immunizing a large number of fish against V harveyi (C) 2008 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordVibrio Harveyi Pseudosciaena Crocea Systemic Antibodies Mucosal Antibodies Antibody Secreting Cells
Department[Xu, Zhen; Chen, Chang-Fu] Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China; [Xu, Zhen; Chen, Chang-Fu] Huazhong Agr Univ, Minist Educ, Key Lab Agr Microbiol, Wuhan 430070, Peoples R China; [Xu, Zhen] Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Mao, Zhi-Juan; Zhu, Wu-Ying] Zhejiang Univ, Coll Biomed Engn & Instrument Sci, Hangzhou 310027, Zhejiang, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1016/j.aquaculture.2008.10.026
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30271032] ; National Natural Science Foundation of China [30271032] ; National Natural Science Foundation of China [30271032] ; National Natural Science Foundation of China [30271032]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000263388500002
WOS KeywordEDWARDSIELLA-TARDA INFECTION ; BASS DICENTRARCHUS-LABRAX ; TROUT ONCORHYNCHUS-MYKISS ; VULNIFICUS ; IMMERSION ; L. ; PISCICIDA ; EXPOSURE ; KINETICS ; EFFICACY
Funding OrganizationNational Natural Science Foundation of China [30271032] ; National Natural Science Foundation of China [30271032] ; National Natural Science Foundation of China [30271032] ; National Natural Science Foundation of China [30271032]
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7868
Collection期刊论文
Corresponding AuthorChen, CF, Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China
Affiliation1.Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Minist Educ, Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
4.Zhejiang Univ, Coll Biomed Engn & Instrument Sci, Hangzhou 310027, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Xu, Zhen,Chen, Chang-Fu,Mao, Zhi-Juan,et al. Detection of serum and mucosal antibody production and antibody secreting cells (ASCs) in large yellow croaker (Pseudosciaena crocea) following vaccination with Vibrio harveyi via different routes[J]. AQUACULTURE,2009,287(3-4):243-247.
APA Xu, Zhen,Chen, Chang-Fu,Mao, Zhi-Juan,Zhu, Wu-Ying,&Chen, CF, Huazhong Agr Univ, Fishery Coll, Wuhan 430070, Peoples R China.(2009).Detection of serum and mucosal antibody production and antibody secreting cells (ASCs) in large yellow croaker (Pseudosciaena crocea) following vaccination with Vibrio harveyi via different routes.AQUACULTURE,287(3-4),243-247.
MLA Xu, Zhen,et al."Detection of serum and mucosal antibody production and antibody secreting cells (ASCs) in large yellow croaker (Pseudosciaena crocea) following vaccination with Vibrio harveyi via different routes".AQUACULTURE 287.3-4(2009):243-247.
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