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Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China
Pan, Xiaojie1,2; Chang, Fengyi1,2; Liu, Yongding1; Li, Dunhai1; Xu, Aihua1,2; Shen, Yinwu1; Huang, Zebo3; Liu, YD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009-02-01
Source PublicationENVIRONMENTAL TOXICOLOGY
ISSN1520-4081
Volume24Issue:1Pages:10-18
AbstractSome species of the genera Anabaena can produce various kinds of cyanotoxins, which may pose risks to environment and human health. Anabaena has frequently been observed in eutrophic freshwater of China in recent years, but its toxicity has been reported only in a few studies. In the present study, the toxicity of an Anabaena flos-aquae strain isolated from Lake Dianchi was investigated. Acute toxicity testing was performed by mouse bioassay using crude extracts from the lyophilized cultures. The mice exposed to crude extracts showed visible symptoms of toxicity and died within 10-24 h of the injection. Serum biochemical parameters were evaluated by the use of commercial diagnostic kits. Significant alterations were found in the serum biochemical parameters: alkaline phosphatase (AKP), gamma-glutamyl transpepticlase (gamma-GT), aspartate amino transferase (AST), alanine amino transferase (ALT), AST/ALT ratio, total protein content, albumin content, albumin/globulin (A/G) ratio, blood urea nitrogen (BUN), serum creatinine (Ssr), and total antioxidative capacity (T-AOC). Histopathological observations were carried out with hematoxylin and eosin (HE) stain under light microscope. Severe lesions were seen in the livers, kidneys, and lungs of the mice injected with crude extracts. The alterations of biochemical parameters were in a dose-dependent manner, and the severities of histological lesions were in the same manner. Based on biochemical and histological studies, this research firstly shows the presence of toxin-producing Anabaena species in Lake Dianchi and the toxic effects of its crude extracts on mammals. (C) 2008 Wiley Periodicals, Inc. Environ Toxicol 24: 10-18, 2009.; Some species of the genera Anabaena can produce various kinds of cyanotoxins, which may pose risks to environment and human health. Anabaena has frequently been observed in eutrophic freshwater of China in recent years, but its toxicity has been reported only in a few studies. In the present study, the toxicity of an Anabaena flos-aquae strain isolated from Lake Dianchi was investigated. Acute toxicity testing was performed by mouse bioassay using crude extracts from the lyophilized cultures. The mice exposed to crude extracts showed visible symptoms of toxicity and died within 10-24 h of the injection. Serum biochemical parameters were evaluated by the use of commercial diagnostic kits. Significant alterations were found in the serum biochemical parameters: alkaline phosphatase (AKP), gamma-glutamyl transpepticlase (gamma-GT), aspartate amino transferase (AST), alanine amino transferase (ALT), AST/ALT ratio, total protein content, albumin content, albumin/globulin (A/G) ratio, blood urea nitrogen (BUN), serum creatinine (Ssr), and total antioxidative capacity (T-AOC). Histopathological observations were carried out with hematoxylin and eosin (HE) stain under light microscope. Severe lesions were seen in the livers, kidneys, and lungs of the mice injected with crude extracts. The alterations of biochemical parameters were in a dose-dependent manner, and the severities of histological lesions were in the same manner. Based on biochemical and histological studies, this research firstly shows the presence of toxin-producing Anabaena species in Lake Dianchi and the toxic effects of its crude extracts on mammals. (C) 2008 Wiley Periodicals, Inc. Environ Toxicol 24: 10-18, 2009.
SubtypeArticle
KeywordToxicity Anabaena Flos-aquae Mouse Bioassay Biochemical Parameters Histopathology Lake Dianchi
Department[Pan, Xiaojie; Chang, Fengyi; Liu, Yongding; Li, Dunhai; Xu, Aihua; Shen, Yinwu] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Pan, Xiaojie; Chang, Fengyi; Xu, Aihua] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Huang, Zebo] Wuhan Univ, Coll Pharm, Wuhan 430072, Peoples R China
Subject AreaEnvironmental Sciences ; Toxicology ; Water Resources
DOI10.1002/tox.20385
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationMinistry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10] ; Ministry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10] ; Ministry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10] ; Ministry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology ; Water Resources
WOS SubjectEnvironmental Sciences ; Toxicology ; Water Resources
WOS IDWOS:000263533000002
WOS KeywordCYLINDROSPERMOPSIS-RACIBORSKII ; TOXIN ; PHORMIDIUM ; STRAINS ; BLOOMS ; LR
Funding OrganizationMinistry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10] ; Ministry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10] ; Ministry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10] ; Ministry of Science and Technology, People's Republic of China [2002CB412300, 2005AA60101504]; Chinese Academy of Sciences [KZCX2-YW-426, KSCX2-1-10]
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7862
Collection期刊论文
Corresponding AuthorLiu, YD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Wuhan Univ, Coll Pharm, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Pan, Xiaojie,Chang, Fengyi,Liu, Yongding,et al. Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China[J]. ENVIRONMENTAL TOXICOLOGY,2009,24(1):10-18.
APA Pan, Xiaojie.,Chang, Fengyi.,Liu, Yongding.,Li, Dunhai.,Xu, Aihua.,...&Liu, YD, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China.ENVIRONMENTAL TOXICOLOGY,24(1),10-18.
MLA Pan, Xiaojie,et al."Mouse Toxicity of Anabaena flos-aquae from Lake Dianchi, China".ENVIRONMENTAL TOXICOLOGY 24.1(2009):10-18.
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