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First Identification of the Hepatotoxic Microcystins in the Serum of a Chronically Exposed Human Population Together with Indication of Hepatocellular Damage
Chen, Jun; Xie, Ping; Li, Li; Xu, Jun; Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
2009-03-01
Source PublicationTOXICOLOGICAL SCIENCES
ISSN1096-6080
Volume108Issue:1Pages:81-89
AbstractHepatotoxic microcystins (MCs) are the most commonly reported cyanotoxins in eutrophic freshwaters. In 1996, human intoxications by MCs caused deaths of 76 patients at Caruaru dialysis centers in Brazil. So far, there have been no direct evidences of MC occurrence in human tissue in consequence of exposure to MC. In this study, we improved cleanup procedures for detecting MCs in serum sample using liquid chromatographymass spectrometry, and confirmed for the first time the presence of MCs in serum samples (average 0.39 ng/ml, which amounts to ca. 1/87 of the concentrations found in tissue samples of the Caruaru victims) of fishermen at Lake Chaohu. Daily intake by the fishermen was estimated to be in the range of 2.2-3.9 mu g MC-LReq, whereas the provisional World Health Organization tolerable daily intake (TDI) for daily lifetime exposure is 0.04 mu g/kg or 2-3 mu g per person. Moreover, statistical analysis showed closer positive relationships between MC serum concentrations and concentrations of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase than between the MC concentrations and other biochemical indicators. Thus, the data raise the question whether extended exposure in the range of the TDI or up to a factor of 10 above it may already lead to indication of liver damage. The results also demonstrate a risk of health effects from chronic exposure to MCs at least for populations with high levels of exposure, like these fishermen.; Hepatotoxic microcystins (MCs) are the most commonly reported cyanotoxins in eutrophic freshwaters. In 1996, human intoxications by MCs caused deaths of 76 patients at Caruaru dialysis centers in Brazil. So far, there have been no direct evidences of MC occurrence in human tissue in consequence of exposure to MC. In this study, we improved cleanup procedures for detecting MCs in serum sample using liquid chromatographymass spectrometry, and confirmed for the first time the presence of MCs in serum samples (average 0.39 ng/ml, which amounts to ca. 1/87 of the concentrations found in tissue samples of the Caruaru victims) of fishermen at Lake Chaohu. Daily intake by the fishermen was estimated to be in the range of 2.2-3.9 mu g MC-LReq, whereas the provisional World Health Organization tolerable daily intake (TDI) for daily lifetime exposure is 0.04 mu g/kg or 2-3 mu g per person. Moreover, statistical analysis showed closer positive relationships between MC serum concentrations and concentrations of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase than between the MC concentrations and other biochemical indicators. Thus, the data raise the question whether extended exposure in the range of the TDI or up to a factor of 10 above it may already lead to indication of liver damage. The results also demonstrate a risk of health effects from chronic exposure to MCs at least for populations with high levels of exposure, like these fishermen.
SubtypeArticle
KeywordSerum Microcystins Serum Biochemical Indices Natural Chronic Exposure Fishermen Hepatocellular Damage
Department[Chen, Jun; Xie, Ping; Li, Li; Xu, Jun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Subject AreaToxicology
DOI10.1093/toxsci/kfp009
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077]
Indexed BySCI
Language英语
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000263606600009
WOS KeywordBIOLOGICAL EVIDENCE ; HUMAN FATALITIES ; LR ; MICE ; BRAZIL ; LIVER ; CARUARU ; INTOXICATION ; TOXINS
Funding OrganizationNational Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077] ; National Basic Research Program of China [2008CB418101]; National Natural Science Foundation of China [30700077]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7850
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Jun,Xie, Ping,Li, Li,et al. First Identification of the Hepatotoxic Microcystins in the Serum of a Chronically Exposed Human Population Together with Indication of Hepatocellular Damage[J]. TOXICOLOGICAL SCIENCES,2009,108(1):81-89.
APA Chen, Jun,Xie, Ping,Li, Li,Xu, Jun,&Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.(2009).First Identification of the Hepatotoxic Microcystins in the Serum of a Chronically Exposed Human Population Together with Indication of Hepatocellular Damage.TOXICOLOGICAL SCIENCES,108(1),81-89.
MLA Chen, Jun,et al."First Identification of the Hepatotoxic Microcystins in the Serum of a Chronically Exposed Human Population Together with Indication of Hepatocellular Damage".TOXICOLOGICAL SCIENCES 108.1(2009):81-89.
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