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Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)
Li, Dunhai1; Xing, Wei2; Li, Genbao1; Liu, Yongding1; Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009-02-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume21Issue:1Pages:119-125
AbstractThere are several apparent developmental stages in the life cycle of Nostoc sphaeroides Kutzing, an edible cyanobacterium found mainly in paddy fields in central China. The cytochemical changes in developmental stages such as hormogonia, aseriate stage, filamentous stage and colony in N. sphaeroides were examined using fluorescent staining and colorimetric methods. The staining of acidic and sulfated polysaccharides increased with development when hormogonia were used as the starting point. Acidic polysaccharides (AP) were most abundant at the aseriate stage and then decreased, while the sulfated polysaccharides (SP) were highest at the colony stage. Quantitatively, along the developmental process from hormogonia to colony, total carbohydrates first increased, then became stable, and then reached their highest level at the colony stage, while reducing sugars were highest at the hormogonia stage and then decreased sharply once development began. SP were not detectable in the hot water soluble polysaccharides (HWSP), and hormogonia had the lowest content of AP, while old colonies had the highest. The AP content of the aseriate stage, filamentous stage and young colony stage were very similar. The evolutionary relationships reflected in the developmental stages of N. sphaeroides are discussed.; There are several apparent developmental stages in the life cycle of Nostoc sphaeroides Kutzing, an edible cyanobacterium found mainly in paddy fields in central China. The cytochemical changes in developmental stages such as hormogonia, aseriate stage, filamentous stage and colony in N. sphaeroides were examined using fluorescent staining and colorimetric methods. The staining of acidic and sulfated polysaccharides increased with development when hormogonia were used as the starting point. Acidic polysaccharides (AP) were most abundant at the aseriate stage and then decreased, while the sulfated polysaccharides (SP) were highest at the colony stage. Quantitatively, along the developmental process from hormogonia to colony, total carbohydrates first increased, then became stable, and then reached their highest level at the colony stage, while reducing sugars were highest at the hormogonia stage and then decreased sharply once development began. SP were not detectable in the hot water soluble polysaccharides (HWSP), and hormogonia had the lowest content of AP, while old colonies had the highest. The AP content of the aseriate stage, filamentous stage and young colony stage were very similar. The evolutionary relationships reflected in the developmental stages of N. sphaeroides are discussed.
SubtypeArticle
KeywordCyanobacterium Cytochemical Changes Development Nostoc Sphaeroides Polysaccharides
Department[Li, Dunhai; Li, Genbao; Liu, Yongding] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Xing, Wei] Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI10.1007/s10811-008-9340-6
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationCAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306] ; CAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306] ; CAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306] ; CAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000263877000015
WOS KeywordBLUE-GREEN-ALGAE ; DIFFERENT COLONY MORPHOLOGIES ; STRUCTURAL-CHARACTERIZATION ; NITROGEN-FIXATION ; POLYSACCHARIDES ; COMMUNE ; ULTRAVIOLET ; SCYTONEMIN ; CULTURES ; PIGMENT
Funding OrganizationCAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306] ; CAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306] ; CAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306] ; CAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306]
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Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7846
Collection期刊论文
Corresponding AuthorLiu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
Recommended Citation
GB/T 7714
Li, Dunhai,Xing, Wei,Li, Genbao,et al. Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)[J]. JOURNAL OF APPLIED PHYCOLOGY,2009,21(1):119-125.
APA Li, Dunhai,Xing, Wei,Li, Genbao,Liu, Yongding,&Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium).JOURNAL OF APPLIED PHYCOLOGY,21(1),119-125.
MLA Li, Dunhai,et al."Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)".JOURNAL OF APPLIED PHYCOLOGY 21.1(2009):119-125.
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