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学科主题: Genetics & Heredity; Virology
题名: Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein
作者: Zhao, Zhe1; Ke, Fei1; Shi, Yan1; Zhou, Guang-1; Gui, Jian-Fang1; Zhang, Qi-Ya1
通讯作者: Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Rana grylio virus (RGV) ; Iridovirus ; Thymidine kinase ; Early viral gene ; Cytoplasmic protein
刊名: VIRUS GENES
发表日期: 2009-04-01
DOI: 10.1007/s11262-008-0318-x
卷: 38, 期:2, 页:345-352
收录类别: SCI
文章类型: Article
部门归属: [Zhao, Zhe; Ke, Fei; Shi, Yan; Zhou, Guang-; Gui, Jian-Fang; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05]
类目[WOS]: Genetics & Heredity ; Virology
研究领域[WOS]: Genetics & Heredity ; Virology
摘要: The presence of thymidine kinase (TK) is a feature of many large DNA viruses. Here, a TK gene homologue was cloned and characterized from Rana grylio virus (RGV), a member of family Iridoviridae. RGV TK encodes a protein of 195 aa with a predicted molecular mass of 22.1 kDa. Homologues of the protein were present in all the currently sequenced iridoviruses, and phylogenetic analysis showed that it was much close to cellular TK type 2 (TK2), deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Subsequently, Western blotting revealed TK expression increased with time from 6 h post-infection in RGV-infected cells. Using drug inhibition analysis by protein synthesis inhibitor (cycloheximide) and DNA replication inhibitor (cytosine arabinofuranoside), RGV TK was classified as the early expression gene during in vitro infection. Subcellular localization by TK-GFP fusion protein expression and immunofluorescence staining showed RGV TK was an exclusively cytoplasmic protein in fish cells. Collectively, current data indicate that RGV TK was an early gene of iridovirus which encoded a cytoplasmic protein in fish cells.
英文摘要: The presence of thymidine kinase (TK) is a feature of many large DNA viruses. Here, a TK gene homologue was cloned and characterized from Rana grylio virus (RGV), a member of family Iridoviridae. RGV TK encodes a protein of 195 aa with a predicted molecular mass of 22.1 kDa. Homologues of the protein were present in all the currently sequenced iridoviruses, and phylogenetic analysis showed that it was much close to cellular TK type 2 (TK2), deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Subsequently, Western blotting revealed TK expression increased with time from 6 h post-infection in RGV-infected cells. Using drug inhibition analysis by protein synthesis inhibitor (cycloheximide) and DNA replication inhibitor (cytosine arabinofuranoside), RGV TK was classified as the early expression gene during in vitro infection. Subcellular localization by TK-GFP fusion protein expression and immunofluorescence staining showed RGV TK was an exclusively cytoplasmic protein in fish cells. Collectively, current data indicate that RGV TK was an early gene of iridovirus which encoded a cytoplasmic protein in fish cells.
关键词[WOS]: HERPES-SIMPLEX-VIRUS ; LYMPHOCYSTIS DISEASE VIRUS ; COMPLETE GENOME SEQUENCE ; CHILO IRIDESCENT VIRUS ; RANAVIRUS FAMILY IRIDOVIRIDAE ; SINGAPORE GROUPER IRIDOVIRUS ; COMPLETE DNA-SEQUENCE ; MAJOR CAPSID PROTEIN ; SPOT SYNDROME VIRUS ; DEOXYRIBONUCLEOSIDE KINASES
语种: 英语
WOS记录号: WOS:000263900500020
ISSN号: 0920-8569
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7844
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhao, Zhe; Ke, Fei; Shi, Yan; Zhou, Guang-; Gui, Jian-Fang; Zhang, Qi-Ya.Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein,VIRUS GENES,2009,38(2):345-352
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