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Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein
Zhao, Zhe; Ke, Fei; Shi, Yan; Zhou, Guang-; Gui, Jian-Fang; Zhang, Qi-Ya; Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009-04-01
Source PublicationVIRUS GENES
ISSN0920-8569
Volume38Issue:2Pages:345-352
AbstractThe presence of thymidine kinase (TK) is a feature of many large DNA viruses. Here, a TK gene homologue was cloned and characterized from Rana grylio virus (RGV), a member of family Iridoviridae. RGV TK encodes a protein of 195 aa with a predicted molecular mass of 22.1 kDa. Homologues of the protein were present in all the currently sequenced iridoviruses, and phylogenetic analysis showed that it was much close to cellular TK type 2 (TK2), deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Subsequently, Western blotting revealed TK expression increased with time from 6 h post-infection in RGV-infected cells. Using drug inhibition analysis by protein synthesis inhibitor (cycloheximide) and DNA replication inhibitor (cytosine arabinofuranoside), RGV TK was classified as the early expression gene during in vitro infection. Subcellular localization by TK-GFP fusion protein expression and immunofluorescence staining showed RGV TK was an exclusively cytoplasmic protein in fish cells. Collectively, current data indicate that RGV TK was an early gene of iridovirus which encoded a cytoplasmic protein in fish cells.; The presence of thymidine kinase (TK) is a feature of many large DNA viruses. Here, a TK gene homologue was cloned and characterized from Rana grylio virus (RGV), a member of family Iridoviridae. RGV TK encodes a protein of 195 aa with a predicted molecular mass of 22.1 kDa. Homologues of the protein were present in all the currently sequenced iridoviruses, and phylogenetic analysis showed that it was much close to cellular TK type 2 (TK2), deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Subsequently, Western blotting revealed TK expression increased with time from 6 h post-infection in RGV-infected cells. Using drug inhibition analysis by protein synthesis inhibitor (cycloheximide) and DNA replication inhibitor (cytosine arabinofuranoside), RGV TK was classified as the early expression gene during in vitro infection. Subcellular localization by TK-GFP fusion protein expression and immunofluorescence staining showed RGV TK was an exclusively cytoplasmic protein in fish cells. Collectively, current data indicate that RGV TK was an early gene of iridovirus which encoded a cytoplasmic protein in fish cells.
SubtypeArticle
KeywordRana Grylio Virus (Rgv) Iridovirus Thymidine Kinase Early Viral Gene Cytoplasmic Protein
Department[Zhao, Zhe; Ke, Fei; Shi, Yan; Zhou, Guang-; Gui, Jian-Fang; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaGenetics & Heredity ; Virology
DOI10.1007/s11262-008-0318-x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05]
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity ; Virology
WOS SubjectGenetics & Heredity ; Virology
WOS IDWOS:000263900500020
WOS KeywordHERPES-SIMPLEX-VIRUS ; LYMPHOCYSTIS DISEASE VIRUS ; COMPLETE GENOME SEQUENCE ; CHILO IRIDESCENT VIRUS ; RANAVIRUS FAMILY IRIDOVIRIDAE ; SINGAPORE GROUPER IRIDOVIRUS ; COMPLETE DNA-SEQUENCE ; MAJOR CAPSID PROTEIN ; SPOT SYNDROME VIRUS ; DEOXYRIBONUCLEOSIDE KINASES
Funding OrganizationNational Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7844
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Zhe,Ke, Fei,Shi, Yan,et al. Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein[J]. VIRUS GENES,2009,38(2):345-352.
APA Zhao, Zhe.,Ke, Fei.,Shi, Yan.,Zhou, Guang-.,Gui, Jian-Fang.,...&Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein.VIRUS GENES,38(2),345-352.
MLA Zhao, Zhe,et al."Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein".VIRUS GENES 38.2(2009):345-352.
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