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Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus
Su, Jianguo1,2; Zhu, Zuoyan1; Wang, Yaping1; Jang, Songhun1; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume26Issue:1Pages:164-170
AbstractArgonaute 2 gene plays a pivotal role in RNAi in many species. Herein is the first report of the cloning and characterization of Argonaute 2 gene in fish. The full-length cDNA of Gobiocypris rarus Argonaute 2 (GrAgo2) consisted of 3073 nucleotides encoding 869 amino acid residues with a calculated molecular weight of 98.499 kDa and an estimated isoelectric point of 9.18. Analysis of the deduced amino acid sequence showed the presence of two signature domains, PAZ and Piwi. RT-PCR analysis indicated that GrAgo2 mRNA expression could be detected in widespread tissues. After infection with grass carp reovirus, GrAgo2 expression was up-regulated from 12 h post-injection (p < 0.05) and returned to control levels at 48 h post-injection (p > 0.05). These data imply that GrAgo2 is involved in antiviral defense in rare minnow. (C) 2008 Published by Elsevier Ltd.; Argonaute 2 gene plays a pivotal role in RNAi in many species. Herein is the first report of the cloning and characterization of Argonaute 2 gene in fish. The full-length cDNA of Gobiocypris rarus Argonaute 2 (GrAgo2) consisted of 3073 nucleotides encoding 869 amino acid residues with a calculated molecular weight of 98.499 kDa and an estimated isoelectric point of 9.18. Analysis of the deduced amino acid sequence showed the presence of two signature domains, PAZ and Piwi. RT-PCR analysis indicated that GrAgo2 mRNA expression could be detected in widespread tissues. After infection with grass carp reovirus, GrAgo2 expression was up-regulated from 12 h post-injection (p < 0.05) and returned to control levels at 48 h post-injection (p > 0.05). These data imply that GrAgo2 is involved in antiviral defense in rare minnow. (C) 2008 Published by Elsevier Ltd.
SubtypeArticle
KeywordRare Minnow (Gobiocypris Rarus) Argonaute 2 Rna Interference Gene Cloning Mrna Expression
Department[Su, Jianguo; Zhu, Zuoyan; Wang, Yaping; Jang, Songhun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Su, Jianguo] NW A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2008.10.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309] ; National Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309] ; National Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309] ; National Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000264015700020
WOS KeywordFAMILY ; EXPRESSION ; IDENTIFICATION ; PROTEINS ; DOMAINS ; COMPLEX
Funding OrganizationNational Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309] ; National Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309] ; National Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309] ; National Natural Science Foundation of China [30740009, 30871917, 30540084]; National Basic Research Program of China [2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; Northwest A&F University in China [01140309, 05ZR096, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology, CAS [2007171309]
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7834
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.NW A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China
Recommended Citation
GB/T 7714
Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,et al. Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(1):164-170.
APA Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,Jang, Songhun,&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus.FISH & SHELLFISH IMMUNOLOGY,26(1),164-170.
MLA Su, Jianguo,et al."Isolation and characterization of Argonaute 2: A key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus".FISH & SHELLFISH IMMUNOLOGY 26.1(2009):164-170.
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