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学科主题: Reproductive Biology
题名: Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization
作者: Luo, Daji1, 2; Hu, Wei2; Chen, Shangping2; Xiao, Yi1; Sun, Yonghua2; Zhu, Zuoyan1, 2
通讯作者: Zhu, ZY, Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
关键词: embryo ; differentially expressed genes ; reprogramming ; somatic cell nuclear transfer ; suppression subtractive hybridization
刊名: BIOLOGY OF REPRODUCTION
发表日期: 2009-04-01
DOI: 10.1095/biolreprod.108.074203
卷: 80, 期:4, 页:674-684
收录类别: SCI
文章类型: Article
部门归属: [Luo, Daji; Xiao, Yi; Zhu, Zuoyan] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China; [Luo, Daji; Hu, Wei; Chen, Shangping; Sun, Yonghua; Zhu, Zuoyan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Development Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540]
类目[WOS]: Reproductive Biology
研究领域[WOS]: Reproductive Biology
摘要: Comparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on Ensembl description and Gene Ontology Consortium annotation, the problems of reprogramming at the dome stage may occur during nuclear remodeling, translation initiation, and regulation of the cell cycle. The importance of regulation from recipient oocytes in cloning should not be underestimated in zebrafish.
英文摘要: Comparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on Ensembl description and Gene Ontology Consortium annotation, the problems of reprogramming at the dome stage may occur during nuclear remodeling, translation initiation, and regulation of the cell cycle. The importance of regulation from recipient oocytes in cloning should not be underestimated in zebrafish.
关键词[WOS]: SOMATIC-CELL NUCLEI ; PLURIPOTENT STEM-CELLS ; MEDAKA ORYZIAS-LATIPES ; TUMOR-SUPPRESSOR ; ENUCLEATED EGGS ; FISH ; P53 ; CYTOPLASM ; OOCYTES ; CLONING
语种: 英语
WOS记录号: WOS:000264437000009
ISSN号: 0006-3363
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7826
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China

Recommended Citation:
Luo, Daji; Hu, Wei; Chen, Shangping; Xiao, Yi; Sun, Yonghua; Zhu, Zuoyan.Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization,BIOLOGY OF REPRODUCTION,2009,80(4):674-684
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