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Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization
Luo, Daji1,2; Hu, Wei2; Chen, Shangping2; Xiao, Yi1; Sun, Yonghua2; Zhu, Zuoyan1,2; Zhu, ZY, Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
2009-04-01
Source PublicationBIOLOGY OF REPRODUCTION
ISSN0006-3363
Volume80Issue:4Pages:674-684
AbstractComparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on Ensembl description and Gene Ontology Consortium annotation, the problems of reprogramming at the dome stage may occur during nuclear remodeling, translation initiation, and regulation of the cell cycle. The importance of regulation from recipient oocytes in cloning should not be underestimated in zebrafish.; Comparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on Ensembl description and Gene Ontology Consortium annotation, the problems of reprogramming at the dome stage may occur during nuclear remodeling, translation initiation, and regulation of the cell cycle. The importance of regulation from recipient oocytes in cloning should not be underestimated in zebrafish.
SubtypeArticle
KeywordEmbryo Differentially Expressed Genes Reprogramming Somatic Cell Nuclear Transfer Suppression Subtractive Hybridization
Department[Luo, Daji; Xiao, Yi; Zhu, Zuoyan] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China; [Luo, Daji; Hu, Wei; Chen, Shangping; Sun, Yonghua; Zhu, Zuoyan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Subject AreaReproductive Biology
DOI10.1095/biolreprod.108.074203
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationDevelopment Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540] ; Development Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540] ; Development Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540] ; Development Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540]
Indexed BySCI
Language英语
WOS Research AreaReproductive Biology
WOS SubjectReproductive Biology
WOS IDWOS:000264437000009
WOS KeywordSOMATIC-CELL NUCLEI ; PLURIPOTENT STEM-CELLS ; MEDAKA ORYZIAS-LATIPES ; TUMOR-SUPPRESSOR ; ENUCLEATED EGGS ; FISH ; P53 ; CYTOPLASM ; OOCYTES ; CLONING
Funding OrganizationDevelopment Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540] ; Development Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540] ; Development Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540] ; Development Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540]
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7826
Collection期刊论文
Corresponding AuthorZhu, ZY, Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Affiliation1.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Luo, Daji,Hu, Wei,Chen, Shangping,et al. Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization[J]. BIOLOGY OF REPRODUCTION,2009,80(4):674-684.
APA Luo, Daji.,Hu, Wei.,Chen, Shangping.,Xiao, Yi.,Sun, Yonghua.,...&Zhu, ZY, Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China.(2009).Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization.BIOLOGY OF REPRODUCTION,80(4),674-684.
MLA Luo, Daji,et al."Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization".BIOLOGY OF REPRODUCTION 80.4(2009):674-684.
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