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Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways
Chang, M. X.; Wang, Y. P.; Nie, P.1; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2009-02-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume26Issue:2Pages:264-274
AbstractInsect PGRPs can function as bacterial recognition molecules triggering proteolytic and/or signal transduction pathways, with the resultant production of antimicrobial peptides. To explore if zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) has such effects, RNA interference (siRNA) and high-density oligonucleotide microarray analysis were used to identify differentially expressed genes regulated by zfPGRP-SC. The mRNA levels for a set of genes involved in Toll-like receptor signaling pathway, such as TLRs, SARM, MyD88, TRAF6 and nuclear factor (NF)-kappa B2 (p100/p52), were examined by quantitative RT-PCR (QT-PCR). The results from the arrays and QT-PCR showed that the expression of 133 genes was involved in signal transduction pathways, which included Toll-like receptor signaling, Wnt signaling, BMP signaling, insulin receptor signaling, TGF-beta signaling, GPCR signaling, small GTPase signaling, second-messenger-mediated signaling, MAPK signaling, JAK/STAT signaling, apoptosis and anti-apoptosis signaling and other signaling cascades. These signaling pathways may connect with each other to form a complex network to regulate not just immune responses but also other processes such as development and apoptosis. When transiently over-expressed in HEK293T cells, zfPGRP-SC inhibited NF-kappa B activity with and without lipopolysacharide (LPS) stimulation. (C) 2008 Elsevier Ltd. All rights reserved.; Insect PGRPs can function as bacterial recognition molecules triggering proteolytic and/or signal transduction pathways, with the resultant production of antimicrobial peptides. To explore if zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) has such effects, RNA interference (siRNA) and high-density oligonucleotide microarray analysis were used to identify differentially expressed genes regulated by zfPGRP-SC. The mRNA levels for a set of genes involved in Toll-like receptor signaling pathway, such as TLRs, SARM, MyD88, TRAF6 and nuclear factor (NF)-kappa B2 (p100/p52), were examined by quantitative RT-PCR (QT-PCR). The results from the arrays and QT-PCR showed that the expression of 133 genes was involved in signal transduction pathways, which included Toll-like receptor signaling, Wnt signaling, BMP signaling, insulin receptor signaling, TGF-beta signaling, GPCR signaling, small GTPase signaling, second-messenger-mediated signaling, MAPK signaling, JAK/STAT signaling, apoptosis and anti-apoptosis signaling and other signaling cascades. These signaling pathways may connect with each other to form a complex network to regulate not just immune responses but also other processes such as development and apoptosis. When transiently over-expressed in HEK293T cells, zfPGRP-SC inhibited NF-kappa B activity with and without lipopolysacharide (LPS) stimulation. (C) 2008 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordZebrafish Peptidoglycan Recognition Protein Pgrp-sc (zfPgrp-sc) Danio Rerio Rna Interference Microarray Signaling Pathways Nf-kappa b
Department[Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2008.11.007
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30500380]; Knowledge Innovation Program of the Chinese Academy of Science [KSCX2-YW-N-021] ; National Natural Science Foundation of China [30500380]; Knowledge Innovation Program of the Chinese Academy of Science [KSCX2-YW-N-021] ; National Natural Science Foundation of China [30500380]; Knowledge Innovation Program of the Chinese Academy of Science [KSCX2-YW-N-021] ; National Natural Science Foundation of China [30500380]; Knowledge Innovation Program of the Chinese Academy of Science [KSCX2-YW-N-021]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000264329800009
WOS KeywordNF-KAPPA-B ; TOLL-LIKE-RECEPTOR ; GRAM-POSITIVE BACTERIA ; MOLECULAR-CLONING ; TYROSINE KINASE ; DROSOPHILA ; PGRP ; ACTIVATION ; EXPRESSION ; FAMILY
Funding OrganizationNational Natural Science Foundation of China [30500380]; Knowledge Innovation Program of the Chinese Academy of Science [KSCX2-YW-N-021] ; National Natural Science Foundation of China [30500380]; Knowledge Innovation Program of the Chinese Academy of Science [KSCX2-YW-N-021] ; National Natural Science Foundation of China [30500380]; Knowledge Innovation Program of the Chinese Academy of Science [KSCX2-YW-N-021] ; National Natural Science Foundation of China [30500380]; Knowledge Innovation Program of the Chinese Academy of Science [KSCX2-YW-N-021]
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7818
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chang, M. X.,Wang, Y. P.,Nie, P.,et al. Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(2):264-274.
APA Chang, M. X.,Wang, Y. P.,Nie, P.,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.(2009).Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways.FISH & SHELLFISH IMMUNOLOGY,26(2),264-274.
MLA Chang, M. X.,et al."Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways".FISH & SHELLFISH IMMUNOLOGY 26.2(2009):264-274.
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