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Predominant expression and cellular distribution of fish Agr2 in renal collecting system
Xia, Jian-Hong; Jiang, Jun; Shi, Yao-Hua; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009-04-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume152Issue:4Pages:397-404
AbstractAnterior gradient 2 (Agr2) genes encode secretory proteins, and play significant roles in anterior-posterior patterning and tumor metastasis. Agr2 transcripts were shown to display quite diverse tissue distribution in different species, and little was known about the cellular localization of Agr2 proteins. In this study, we identified an Agr2 homologue from gibe[ carp (Carassius auratus gibelio), and revealed the expression patterns and cellular localization during embryogenesis and in adult tissues. The full-length cDNA of CagAgr2 is 803 nucleotides (nt) with an open reading frame of 510 nt encoding 169 amino acids. The Agr2 C-terminus matches to the class I PDZ-interacting motif, suggesting that it might be a PDZ-binding protein. During embryogenesis, CagAgr2 was found to be transcribed in the mucus-secreting hatching gland from tailbud stage and later in the pharynx region, swim bladder and pronephric duct as revealed by RT-PCR and whole mount in situ hybridization. In the adult fish, its transcription was predominantly confined to the kidney, and lower transcription levels were also found in the intestine, ovary and gills. To further localize the Agr2 protein, the anti-CagAgr2 polyclonal antibody was produced and used for immunofluorescence observation. In agreement with mRNA expression data, the Agr2 protein was localized in the pronephric duct of 3dph larvae. In adult fish, Agr2 protein expression is confined to the renal collecting system with asymmetric distribution along the apical-basolateral axis. The data provided suggestive evidence that fish Agr2 might be involved in differentiation and secretory functions of kidney epithelium. (C) 2009 Elsevier Inc. All rights reserved.; Anterior gradient 2 (Agr2) genes encode secretory proteins, and play significant roles in anterior-posterior patterning and tumor metastasis. Agr2 transcripts were shown to display quite diverse tissue distribution in different species, and little was known about the cellular localization of Agr2 proteins. In this study, we identified an Agr2 homologue from gibe[ carp (Carassius auratus gibelio), and revealed the expression patterns and cellular localization during embryogenesis and in adult tissues. The full-length cDNA of CagAgr2 is 803 nucleotides (nt) with an open reading frame of 510 nt encoding 169 amino acids. The Agr2 C-terminus matches to the class I PDZ-interacting motif, suggesting that it might be a PDZ-binding protein. During embryogenesis, CagAgr2 was found to be transcribed in the mucus-secreting hatching gland from tailbud stage and later in the pharynx region, swim bladder and pronephric duct as revealed by RT-PCR and whole mount in situ hybridization. In the adult fish, its transcription was predominantly confined to the kidney, and lower transcription levels were also found in the intestine, ovary and gills. To further localize the Agr2 protein, the anti-CagAgr2 polyclonal antibody was produced and used for immunofluorescence observation. In agreement with mRNA expression data, the Agr2 protein was localized in the pronephric duct of 3dph larvae. In adult fish, Agr2 protein expression is confined to the renal collecting system with asymmetric distribution along the apical-basolateral axis. The data provided suggestive evidence that fish Agr2 might be involved in differentiation and secretory functions of kidney epithelium. (C) 2009 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordAnterior Gradient 2 (Agr2) Kidney Renal Collecting System Epithelium Polarity Gibel Carp
Department[Xia, Jian-Hong; Jiang, Jun; Shi, Yao-Hua; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Zoology
DOI10.1016/j.cbpb.2009.01.014
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117401]; Innovation Project of the Chinese Academy of Sciences [KSCX2-YW-N-020]; Innovation Project of State Key Laboratory of Freshwater Ecology and Biotechnology [085B15-1-F01]; Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Innovation Project of the Chinese Academy of Sciences [KSCX2-YW-N-020]; Innovation Project of State Key Laboratory of Freshwater Ecology and Biotechnology [085B15-1-F01]; Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Innovation Project of the Chinese Academy of Sciences [KSCX2-YW-N-020]; Innovation Project of State Key Laboratory of Freshwater Ecology and Biotechnology [085B15-1-F01]; Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Innovation Project of the Chinese Academy of Sciences [KSCX2-YW-N-020]; Innovation Project of State Key Laboratory of Freshwater Ecology and Biotechnology [085B15-1-F01]; Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS IDWOS:000264616400014
WOS KeywordSILVER CRUCIAN CARP ; XENOPUS CEMENT GLAND ; PDZ-DOMAIN ; CARASSIUS-AURATUS ; BREAST-CANCER ; GENE XAG-2 ; PROTEIN ; KIDNEY ; EMBRYOGENESIS ; LAEVIS
Funding OrganizationNational Major Basic Research Program [2004CB117401]; Innovation Project of the Chinese Academy of Sciences [KSCX2-YW-N-020]; Innovation Project of State Key Laboratory of Freshwater Ecology and Biotechnology [085B15-1-F01]; Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Innovation Project of the Chinese Academy of Sciences [KSCX2-YW-N-020]; Innovation Project of State Key Laboratory of Freshwater Ecology and Biotechnology [085B15-1-F01]; Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Innovation Project of the Chinese Academy of Sciences [KSCX2-YW-N-020]; Innovation Project of State Key Laboratory of Freshwater Ecology and Biotechnology [085B15-1-F01]; Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Innovation Project of the Chinese Academy of Sciences [KSCX2-YW-N-020]; Innovation Project of State Key Laboratory of Freshwater Ecology and Biotechnology [085B15-1-F01]; Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301]
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7806
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xia, Jian-Hong,Jiang, Jun,Shi, Yao-Hua,et al. Predominant expression and cellular distribution of fish Agr2 in renal collecting system[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2009,152(4):397-404.
APA Xia, Jian-Hong,Jiang, Jun,Shi, Yao-Hua,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Predominant expression and cellular distribution of fish Agr2 in renal collecting system.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,152(4),397-404.
MLA Xia, Jian-Hong,et al."Predominant expression and cellular distribution of fish Agr2 in renal collecting system".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 152.4(2009):397-404.
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