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Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)
Li, Yuan1,2; Sun, Baojian1; Wu, Hongjuan2; Nie, Pin1; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2009-04-03
Source PublicationAQUACULTURE
ISSN0044-8486
Volume289Issue:1-2Pages:154-160
AbstractMicrocystins (MCs) are cyanobacterial toxins in water blooms that have received increasing attention as a public biohazard for human and animal health. Previous studies were mainly focused on the toxic effects on adult fish, rather than juvenile or larvae, and the response of fish immune system were usually neglected. This paper presents the first data of the effects of microcystin-LR (MC-LR) on transcription of several genes essential for early lymphoid development (Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha) and heat shock proteins (HSP90, HSP70, HSP60, HSP27) in zebrafish larvae. Relative changes of mRNA transcription were analyzed by real time PCR. The transcription of Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha were up-regulated when following exposure to 800 mu g/L MC-LR, which may indicate that specific lymphocytes differentiation and TCR/lg arrangement are induced to counteract the toxic effects of MC-LR. It was also interesting to note the dramatically increased transcription of HSP90. HSP70, HSP60 and HSP27, which may indicate their important roles as molecular chaperones under oxidative stress. (C) 2009 Elsevier B.V. All rights reserved.; Microcystins (MCs) are cyanobacterial toxins in water blooms that have received increasing attention as a public biohazard for human and animal health. Previous studies were mainly focused on the toxic effects on adult fish, rather than juvenile or larvae, and the response of fish immune system were usually neglected. This paper presents the first data of the effects of microcystin-LR (MC-LR) on transcription of several genes essential for early lymphoid development (Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha) and heat shock proteins (HSP90, HSP70, HSP60, HSP27) in zebrafish larvae. Relative changes of mRNA transcription were analyzed by real time PCR. The transcription of Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha were up-regulated when following exposure to 800 mu g/L MC-LR, which may indicate that specific lymphocytes differentiation and TCR/lg arrangement are induced to counteract the toxic effects of MC-LR. It was also interesting to note the dramatically increased transcription of HSP90. HSP70, HSP60 and HSP27, which may indicate their important roles as molecular chaperones under oxidative stress. (C) 2009 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicrocystin Immunotoxin t Cell Development Heat Shock Proteins Gene Expression Zebrafish
Department[Li, Yuan; Sun, Baojian; Nie, Pin] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Li, Yuan; Wu, Hongjuan] Huazhong Univ Sci & Technol, Coll Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1016/j.aquaculture.2008.12.029
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [20577065, 20747003] ; National Natural Science Foundation of China [20577065, 20747003] ; National Natural Science Foundation of China [20577065, 20747003] ; National Natural Science Foundation of China [20577065, 20747003]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000264942400025
WOS KeywordOXIDATIVE STRESS ; HEPATIC HEAT-SHOCK-PROTEIN-70 ; CYANOBACTERIAL TOXINS ; BETA-NAPHTHOFLAVONE ; MOUSE-LIVER ; DNA-DAMAGE ; IN-VIVO ; EXPRESSION ; APOPTOSIS ; FISH
Funding OrganizationNational Natural Science Foundation of China [20577065, 20747003] ; National Natural Science Foundation of China [20577065, 20747003] ; National Natural Science Foundation of China [20577065, 20747003] ; National Natural Science Foundation of China [20577065, 20747003]
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7794
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2.Huazhong Univ Sci & Technol, Coll Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Li, Yuan,Sun, Baojian,Wu, Hongjuan,et al. Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)[J]. AQUACULTURE,2009,289(1-2):154-160.
APA Li, Yuan,Sun, Baojian,Wu, Hongjuan,Nie, Pin,&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.(2009).Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio).AQUACULTURE,289(1-2),154-160.
MLA Li, Yuan,et al."Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)".AQUACULTURE 289.1-2(2009):154-160.
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