| Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus | |
| Su, Jianguo1,2; Zhu, Zuoyan1; Wang, Yaping1; Zou, Jun3; Wang, Na1; Jang, Songhun1; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Peoples R China | |
| 2009-04-03 | |
| Source Publication | AQUACULTURE
 |
| ISSN | 0044-8486 |
| Volume | 289Issue:1-2Pages:1-5 |
| Abstract | Dicer catalyzes the initiation step of RNA interference (RNAi) which is known to play a significant role in innate immune response to viral infection in many organisms. To study the RNAi-related pathway after virus infection in fish, we identified a partial cDNA sequence of dicer from rare minnow, Gobiocypris rants. Real-time quantitative RT-PCR (qRT-PCR) demonstrated the Dicer transcript level was the highest at zygote stage, decreased at prim-5 stage, and was stable from the protruding mouth to adult stage. Regular RT-PCR analysis showed that the Dicer gene expressed widely in the tested tissues, including brain, gill, heart, intestine, kidney, liver, muscle, ovary, spleen and testis. The expression of Dicer mRNA was significantly increased in the early period of Grass carp reovirus (GCRV) infection, and declined from 24 It post-injection (h p.i.) (P<0.05). The mRNA expression returned to control levels at 48 h p.i. (P>0.05). Under transmission electron microscope, virions were difficulty to find out in 12 h p.i., and virus inclusion bodies and few scattered viral particles were easily visualized from 24 h p.i. to moribund. These results implied GCRV triggered the RNAi pathway in the early stages of infection and perhaps virus inclusion bodies suppressed the antiviral functions of RNAi mechanism. (C) 2009 Published by Elsevier B.V.; Dicer catalyzes the initiation step of RNA interference (RNAi) which is known to play a significant role in innate immune response to viral infection in many organisms. To study the RNAi-related pathway after virus infection in fish, we identified a partial cDNA sequence of dicer from rare minnow, Gobiocypris rants. Real-time quantitative RT-PCR (qRT-PCR) demonstrated the Dicer transcript level was the highest at zygote stage, decreased at prim-5 stage, and was stable from the protruding mouth to adult stage. Regular RT-PCR analysis showed that the Dicer gene expressed widely in the tested tissues, including brain, gill, heart, intestine, kidney, liver, muscle, ovary, spleen and testis. The expression of Dicer mRNA was significantly increased in the early period of Grass carp reovirus (GCRV) infection, and declined from 24 It post-injection (h p.i.) (P<0.05). The mRNA expression returned to control levels at 48 h p.i. (P>0.05). Under transmission electron microscope, virions were difficulty to find out in 12 h p.i., and virus inclusion bodies and few scattered viral particles were easily visualized from 24 h p.i. to moribund. These results implied GCRV triggered the RNAi pathway in the early stages of infection and perhaps virus inclusion bodies suppressed the antiviral functions of RNAi mechanism. (C) 2009 Published by Elsevier B.V. |
| Subtype | Article |
| Keyword | Gobiocypris Torus Grass Carp Reovirus Rna Interference Dicer Mrna Expression Virus Inclusion Body |
| Department | [Su, Jianguo; Zhu, Zuoyan; Wang, Yaping; Wang, Na; Jang, Songhun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Su, Jianguo] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Coll Anim Sci & Technol, Yangling 712100, Peoples R China; [Zou, Jun] Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland |
| Subject Area | Fisheries ; Marine & Freshwater Biology |
| DOI | 10.1016/j.aquaculture.2008.12.027 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000264942400001 |
| WOS Keyword | INTERFERENCE PATHWAY ; DICER ; VIRUSES ; MORPHOGENESIS ; DEGRADATION ; TRANSCRIPTS ; DROSOPHILA ; PROTEINS ; DOMAIN ; DSRNA |
| Funding Organization | National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7792 |
| Collection | 期刊论文 |
| Corresponding Author | Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 3.Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland |
| Recommended Citation GB/T 7714 | Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,et al. Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus[J]. AQUACULTURE,2009,289(1-2):1-5. |
| APA | Su, Jianguo.,Zhu, Zuoyan.,Wang, Yaping.,Zou, Jun.,Wang, Na.,...&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Peoples R China.(2009).Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus.AQUACULTURE,289(1-2),1-5. |
| MLA | Su, Jianguo,et al."Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus".AQUACULTURE 289.1-2(2009):1-5. |
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