IHB OpenIR  > 期刊论文
Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus
Su, Jianguo1,2; Zhu, Zuoyan1; Wang, Yaping1; Zou, Jun3; Wang, Na1; Jang, Songhun1; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Peoples R China
2009-04-03
Source PublicationAQUACULTURE
ISSN0044-8486
Volume289Issue:1-2Pages:1-5
AbstractDicer catalyzes the initiation step of RNA interference (RNAi) which is known to play a significant role in innate immune response to viral infection in many organisms. To study the RNAi-related pathway after virus infection in fish, we identified a partial cDNA sequence of dicer from rare minnow, Gobiocypris rants. Real-time quantitative RT-PCR (qRT-PCR) demonstrated the Dicer transcript level was the highest at zygote stage, decreased at prim-5 stage, and was stable from the protruding mouth to adult stage. Regular RT-PCR analysis showed that the Dicer gene expressed widely in the tested tissues, including brain, gill, heart, intestine, kidney, liver, muscle, ovary, spleen and testis. The expression of Dicer mRNA was significantly increased in the early period of Grass carp reovirus (GCRV) infection, and declined from 24 It post-injection (h p.i.) (P<0.05). The mRNA expression returned to control levels at 48 h p.i. (P>0.05). Under transmission electron microscope, virions were difficulty to find out in 12 h p.i., and virus inclusion bodies and few scattered viral particles were easily visualized from 24 h p.i. to moribund. These results implied GCRV triggered the RNAi pathway in the early stages of infection and perhaps virus inclusion bodies suppressed the antiviral functions of RNAi mechanism. (C) 2009 Published by Elsevier B.V.; Dicer catalyzes the initiation step of RNA interference (RNAi) which is known to play a significant role in innate immune response to viral infection in many organisms. To study the RNAi-related pathway after virus infection in fish, we identified a partial cDNA sequence of dicer from rare minnow, Gobiocypris rants. Real-time quantitative RT-PCR (qRT-PCR) demonstrated the Dicer transcript level was the highest at zygote stage, decreased at prim-5 stage, and was stable from the protruding mouth to adult stage. Regular RT-PCR analysis showed that the Dicer gene expressed widely in the tested tissues, including brain, gill, heart, intestine, kidney, liver, muscle, ovary, spleen and testis. The expression of Dicer mRNA was significantly increased in the early period of Grass carp reovirus (GCRV) infection, and declined from 24 It post-injection (h p.i.) (P<0.05). The mRNA expression returned to control levels at 48 h p.i. (P>0.05). Under transmission electron microscope, virions were difficulty to find out in 12 h p.i., and virus inclusion bodies and few scattered viral particles were easily visualized from 24 h p.i. to moribund. These results implied GCRV triggered the RNAi pathway in the early stages of infection and perhaps virus inclusion bodies suppressed the antiviral functions of RNAi mechanism. (C) 2009 Published by Elsevier B.V.
SubtypeArticle
KeywordGobiocypris Torus Grass Carp Reovirus Rna Interference Dicer Mrna Expression Virus Inclusion Body
Department[Su, Jianguo; Zhu, Zuoyan; Wang, Yaping; Wang, Na; Jang, Songhun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Su, Jianguo] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Coll Anim Sci & Technol, Yangling 712100, Peoples R China; [Zou, Jun] Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1016/j.aquaculture.2008.12.027
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000264942400001
WOS KeywordINTERFERENCE PATHWAY ; DICER ; VIRUSES ; MORPHOGENESIS ; DEGRADATION ; TRANSCRIPTS ; DROSOPHILA ; PROTEINS ; DOMAIN ; DSRNA
Funding OrganizationNational Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298] ; National Natural Science Foundation of China [30871917, 30740009, 30540084]; 973 National Basic Research Program of China [973 National Basic Research Program of China (2006CB102100]; Chinese Academy of Sciences [KSCX2-YW-N-021]; SRF [14110104]; Northwest A & F University in China [01140309, 08080113]; China Postdoctoral Science Foundation [20070410298]
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7792
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
3.Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland
Recommended Citation
GB/T 7714
Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,et al. Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus[J]. AQUACULTURE,2009,289(1-2):1-5.
APA Su, Jianguo.,Zhu, Zuoyan.,Wang, Yaping.,Zou, Jun.,Wang, Na.,...&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Peoples R China.(2009).Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus.AQUACULTURE,289(1-2),1-5.
MLA Su, Jianguo,et al."Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus".AQUACULTURE 289.1-2(2009):1-5.
Files in This Item:
File Name/Size DocType Version Access License
Grass carp reovirus (744KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Su, Jianguo]'s Articles
[Zhu, Zuoyan]'s Articles
[Wang, Yaping]'s Articles
Baidu academic
Similar articles in Baidu academic
[Su, Jianguo]'s Articles
[Zhu, Zuoyan]'s Articles
[Wang, Yaping]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Su, Jianguo]'s Articles
[Zhu, Zuoyan]'s Articles
[Wang, Yaping]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.