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Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts
Chang, Mingxian1,2; Nie, Pin2; Collet, Bertrand3; Secombes, Christopher J.1; Zou, Jun1; Zou, J, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Zool Bldg, Aberdeen AB24 2TZ, Scotland
2009-04-01
Source PublicationIMMUNOGENETICS
ISSN0093-7711
Volume61Issue:4Pages:315-325
AbstractMultiple type I interferons (IFNs) have recently been identified in salmonids, containing two or four conserved cysteines. In this work, a novel two-cysteine containing (2C) IFN gene was identified in rainbow trout. This novel trout IFN gene (termed IFN5) formed a phylogenetic group that is distinct from the other three salmonid IFN groups sequenced to date and had a close evolutionary relationship with IFNs from advanced fish species. Our data demonstrate that two subgroups are apparent within each of the 2C and 4C type I IFNs, an evolutionary outcome possibly due to two rounds of genome duplication events that have occurred within teleosts. We have examined gene expression of the trout 2C type I IFN in cultured cells following stimulation with lipopolysaccharide, phytohaemagglutinin, polyI:C or recombinant IFN, or after transfection with polyI:C. The kinetics of gene expression was also studied after viral infection. Analysis of the regulatory elements in the IFN promoter region predicted several binding sites for key transcription factors that potentially play an important role in mediating IFN5 gene expression.; Multiple type I interferons (IFNs) have recently been identified in salmonids, containing two or four conserved cysteines. In this work, a novel two-cysteine containing (2C) IFN gene was identified in rainbow trout. This novel trout IFN gene (termed IFN5) formed a phylogenetic group that is distinct from the other three salmonid IFN groups sequenced to date and had a close evolutionary relationship with IFNs from advanced fish species. Our data demonstrate that two subgroups are apparent within each of the 2C and 4C type I IFNs, an evolutionary outcome possibly due to two rounds of genome duplication events that have occurred within teleosts. We have examined gene expression of the trout 2C type I IFN in cultured cells following stimulation with lipopolysaccharide, phytohaemagglutinin, polyI:C or recombinant IFN, or after transfection with polyI:C. The kinetics of gene expression was also studied after viral infection. Analysis of the regulatory elements in the IFN promoter region predicted several binding sites for key transcription factors that potentially play an important role in mediating IFN5 gene expression.
SubtypeArticle
KeywordInterferon Evolution Antiviral Defence Rainbow Trout Teleosts
Department[Chang, Mingxian; Secombes, Christopher J.; Zou, Jun] Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland; [Chang, Mingxian; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Collet, Bertrand] Fisheries Res Serv, Marine Lab, Aberdeen AB11 9DK, Scotland
Subject AreaGenetics & Heredity ; Immunology
DOI10.1007/s00251-009-0366-y
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationRoyal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission ; Royal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission ; Royal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission ; Royal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity ; Immunology
WOS SubjectGenetics & Heredity ; Immunology
WOS IDWOS:000264947800007
WOS KeywordSALMON-ANEMIA-VIRUS ; ATLANTIC SALMON ; GENOME DUPLICATION ; DANIO-RERIO ; CELL-LINE ; FISH ; EXPRESSION ; INFECTION ; PHYTOHEMAGGLUTININ ; ZEBRAFISH
Funding OrganizationRoyal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission ; Royal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission ; Royal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission ; Royal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission
Citation statistics
Cited Times:55[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7790
Collection期刊论文
Corresponding AuthorZou, J, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Zool Bldg, Aberdeen AB24 2TZ, Scotland
Affiliation1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Fisheries Res Serv, Marine Lab, Aberdeen AB11 9DK, Scotland
Recommended Citation
GB/T 7714
Chang, Mingxian,Nie, Pin,Collet, Bertrand,et al. Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts[J]. IMMUNOGENETICS,2009,61(4):315-325.
APA Chang, Mingxian,Nie, Pin,Collet, Bertrand,Secombes, Christopher J.,Zou, Jun,&Zou, J, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Zool Bldg, Aberdeen AB24 2TZ, Scotland.(2009).Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts.IMMUNOGENETICS,61(4),315-325.
MLA Chang, Mingxian,et al."Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts".IMMUNOGENETICS 61.4(2009):315-325.
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