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学科主题: Genetics & Heredity; Immunology
题名: Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts
作者: Chang, Mingxian1, 2; Nie, Pin2; Collet, Bertrand3; Secombes, Christopher J.1; Zou, Jun1
通讯作者: Zou, J, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Zool Bldg, Aberdeen AB24 2TZ, Scotland
关键词: Interferon ; Evolution ; Antiviral defence ; Rainbow trout ; Teleosts
刊名: IMMUNOGENETICS
发表日期: 2009-04-01
DOI: 10.1007/s00251-009-0366-y
卷: 61, 期:4, 页:315-325
收录类别: SCI
文章类型: Article
部门归属: [Chang, Mingxian; Secombes, Christopher J.; Zou, Jun] Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland; [Chang, Mingxian; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Collet, Bertrand] Fisheries Res Serv, Marine Lab, Aberdeen AB11 9DK, Scotland
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Royal Society of Edinburgh ; National Natural Science Foundation of China [30711130225, 30830083]; European Commission
类目[WOS]: Genetics & Heredity ; Immunology
研究领域[WOS]: Genetics & Heredity ; Immunology
摘要: Multiple type I interferons (IFNs) have recently been identified in salmonids, containing two or four conserved cysteines. In this work, a novel two-cysteine containing (2C) IFN gene was identified in rainbow trout. This novel trout IFN gene (termed IFN5) formed a phylogenetic group that is distinct from the other three salmonid IFN groups sequenced to date and had a close evolutionary relationship with IFNs from advanced fish species. Our data demonstrate that two subgroups are apparent within each of the 2C and 4C type I IFNs, an evolutionary outcome possibly due to two rounds of genome duplication events that have occurred within teleosts. We have examined gene expression of the trout 2C type I IFN in cultured cells following stimulation with lipopolysaccharide, phytohaemagglutinin, polyI:C or recombinant IFN, or after transfection with polyI:C. The kinetics of gene expression was also studied after viral infection. Analysis of the regulatory elements in the IFN promoter region predicted several binding sites for key transcription factors that potentially play an important role in mediating IFN5 gene expression.
英文摘要: Multiple type I interferons (IFNs) have recently been identified in salmonids, containing two or four conserved cysteines. In this work, a novel two-cysteine containing (2C) IFN gene was identified in rainbow trout. This novel trout IFN gene (termed IFN5) formed a phylogenetic group that is distinct from the other three salmonid IFN groups sequenced to date and had a close evolutionary relationship with IFNs from advanced fish species. Our data demonstrate that two subgroups are apparent within each of the 2C and 4C type I IFNs, an evolutionary outcome possibly due to two rounds of genome duplication events that have occurred within teleosts. We have examined gene expression of the trout 2C type I IFN in cultured cells following stimulation with lipopolysaccharide, phytohaemagglutinin, polyI:C or recombinant IFN, or after transfection with polyI:C. The kinetics of gene expression was also studied after viral infection. Analysis of the regulatory elements in the IFN promoter region predicted several binding sites for key transcription factors that potentially play an important role in mediating IFN5 gene expression.
关键词[WOS]: SALMON-ANEMIA-VIRUS ; ATLANTIC SALMON ; GENOME DUPLICATION ; DANIO-RERIO ; CELL-LINE ; FISH ; EXPRESSION ; INFECTION ; PHYTOHEMAGGLUTININ ; ZEBRAFISH
语种: 英语
WOS记录号: WOS:000264947800007
ISSN号: 0093-7711
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7790
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Fisheries Res Serv, Marine Lab, Aberdeen AB11 9DK, Scotland

Recommended Citation:
Chang, Mingxian; Nie, Pin; Collet, Bertrand; Secombes, Christopher J.; Zou, Jun.Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts,IMMUNOGENETICS,2009,61(4):315-325
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