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学科主题: Ecology; Marine & Freshwater Biology; Microbiology
题名: Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China
作者: Gao, E-Bin1; Yuan, Xiu-Ping1; Li, Ren-Hui1; Zhang, Qi-Ya1
通讯作者: Zhang, QY, Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Cyanophage ; Planktothrix agardhii ; Viral infection ; Blooms ; Cyanobacteria ; Freshwater lake
刊名: AQUATIC MICROBIAL ECOLOGY
发表日期: 2009
DOI: 10.3354/ame01266
卷: 54, 期:2, 页:163-170
收录类别: SCI
文章类型: Article
部门归属: [Gao, E-Bin; Yuan, Xiu-Ping; Li, Ren-Hui; Zhang, Qi-Ya] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008]
类目[WOS]: Ecology ; Marine & Freshwater Biology ; Microbiology
研究领域[WOS]: Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
摘要: A previously unknown cyanophage, PaV-LD (Planktothrix agardhii Virus isolated from Lake Donghu), which causes lysis of the bloom-forming filamentous cyanobacterium P. agardhii, was isolated from Lake Donghu, Wuhan, China. PaV-LD only lysed P. agardhii strains isolated from Lake Donghu and not those isolated from other lakes. The PaV-LD particle has an icosahedral, non-tailed structure, ca. 70 to 85 nm (mean +/- SD = 76 +/- 6 nm) in diameter. PaV-LD was stable at freezing temperature, but lost its infectivity at temperatures >50 degrees C. Lysis of host cells was delayed about 3 d after the PaV-LD treatment with chloroform, and the virus was inactivated by exposure to low pH (<= 4). The latent period and burst size of the PaV-LD were estimated to be 48 to 72 h and about 340 infectious units per cell, respectively. The regrowth cultures of surviving host filaments were not lysed by the PaV-LD suspension. To our knowledge, this is the first isolation and cultivation of a virus infectious to the filamentous bloom-forming cyanobacterium Planktothrix from a freshwater lake.
英文摘要: A previously unknown cyanophage, PaV-LD (Planktothrix agardhii Virus isolated from Lake Donghu), which causes lysis of the bloom-forming filamentous cyanobacterium P. agardhii, was isolated from Lake Donghu, Wuhan, China. PaV-LD only lysed P. agardhii strains isolated from Lake Donghu and not those isolated from other lakes. The PaV-LD particle has an icosahedral, non-tailed structure, ca. 70 to 85 nm (mean +/- SD = 76 +/- 6 nm) in diameter. PaV-LD was stable at freezing temperature, but lost its infectivity at temperatures >50 degrees C. Lysis of host cells was delayed about 3 d after the PaV-LD treatment with chloroform, and the virus was inactivated by exposure to low pH (<= 4). The latent period and burst size of the PaV-LD were estimated to be 48 to 72 h and about 340 infectious units per cell, respectively. The regrowth cultures of surviving host filaments were not lysed by the PaV-LD suspension. To our knowledge, this is the first isolation and cultivation of a virus infectious to the filamentous bloom-forming cyanobacterium Planktothrix from a freshwater lake.
关键词[WOS]: MICROCYSTIS-AERUGINOSA ; MARINE SYNECHOCOCCUS ; BLOOM ; VIRUSES ; DIVERSITY ; WATER ; VIRIOPLANKTON ; COMMUNITIES ; DYNAMICS ; SHALLOW
语种: 英语
WOS记录号: WOS:000265158200005
ISSN号: 0948-3055
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7786
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Gao, E-Bin; Yuan, Xiu-Ping; Li, Ren-Hui; Zhang, Qi-Ya.Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China,AQUATIC MICROBIAL ECOLOGY,2009,54(2):163-170
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