IHB OpenIR  > 期刊论文
Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China
Gao, E-Bin; Yuan, Xiu-Ping; Li, Ren-Hui; Zhang, Qi-Ya; Zhang, QY, Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009
Source PublicationAQUATIC MICROBIAL ECOLOGY
ISSN0948-3055
Volume54Issue:2Pages:163-170
AbstractA previously unknown cyanophage, PaV-LD (Planktothrix agardhii Virus isolated from Lake Donghu), which causes lysis of the bloom-forming filamentous cyanobacterium P. agardhii, was isolated from Lake Donghu, Wuhan, China. PaV-LD only lysed P. agardhii strains isolated from Lake Donghu and not those isolated from other lakes. The PaV-LD particle has an icosahedral, non-tailed structure, ca. 70 to 85 nm (mean +/- SD = 76 +/- 6 nm) in diameter. PaV-LD was stable at freezing temperature, but lost its infectivity at temperatures >50 degrees C. Lysis of host cells was delayed about 3 d after the PaV-LD treatment with chloroform, and the virus was inactivated by exposure to low pH (<= 4). The latent period and burst size of the PaV-LD were estimated to be 48 to 72 h and about 340 infectious units per cell, respectively. The regrowth cultures of surviving host filaments were not lysed by the PaV-LD suspension. To our knowledge, this is the first isolation and cultivation of a virus infectious to the filamentous bloom-forming cyanobacterium Planktothrix from a freshwater lake.; A previously unknown cyanophage, PaV-LD (Planktothrix agardhii Virus isolated from Lake Donghu), which causes lysis of the bloom-forming filamentous cyanobacterium P. agardhii, was isolated from Lake Donghu, Wuhan, China. PaV-LD only lysed P. agardhii strains isolated from Lake Donghu and not those isolated from other lakes. The PaV-LD particle has an icosahedral, non-tailed structure, ca. 70 to 85 nm (mean +/- SD = 76 +/- 6 nm) in diameter. PaV-LD was stable at freezing temperature, but lost its infectivity at temperatures >50 degrees C. Lysis of host cells was delayed about 3 d after the PaV-LD treatment with chloroform, and the virus was inactivated by exposure to low pH (<= 4). The latent period and burst size of the PaV-LD were estimated to be 48 to 72 h and about 340 infectious units per cell, respectively. The regrowth cultures of surviving host filaments were not lysed by the PaV-LD suspension. To our knowledge, this is the first isolation and cultivation of a virus infectious to the filamentous bloom-forming cyanobacterium Planktothrix from a freshwater lake.
SubtypeArticle
KeywordCyanophage Planktothrix Agardhii Viral Infection Blooms Cyanobacteria Freshwater Lake
Department[Gao, E-Bin; Yuan, Xiu-Ping; Li, Ren-Hui; Zhang, Qi-Ya] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaEcology ; Marine & Freshwater Biology ; Microbiology
DOI10.3354/ame01266
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
WOS SubjectEcology ; Marine & Freshwater Biology ; Microbiology
WOS IDWOS:000265158200005
WOS KeywordMICROCYSTIS-AERUGINOSA ; MARINE SYNECHOCOCCUS ; BLOOM ; VIRUSES ; DIVERSITY ; WATER ; VIRIOPLANKTON ; COMMUNITIES ; DYNAMICS ; SHALLOW
Funding OrganizationNational Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008] ; National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008]
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7786
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Gao, E-Bin,Yuan, Xiu-Ping,Li, Ren-Hui,et al. Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China[J]. AQUATIC MICROBIAL ECOLOGY,2009,54(2):163-170.
APA Gao, E-Bin,Yuan, Xiu-Ping,Li, Ren-Hui,Zhang, Qi-Ya,&Zhang, QY, Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China.AQUATIC MICROBIAL ECOLOGY,54(2),163-170.
MLA Gao, E-Bin,et al."Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China".AQUATIC MICROBIAL ECOLOGY 54.2(2009):163-170.
Files in This Item:
File Name/Size DocType Version Access License
09-Gao et al-Aquat M(649KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Gao, E-Bin]'s Articles
[Yuan, Xiu-Ping]'s Articles
[Li, Ren-Hui]'s Articles
Baidu academic
Similar articles in Baidu academic
[Gao, E-Bin]'s Articles
[Yuan, Xiu-Ping]'s Articles
[Li, Ren-Hui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Gao, E-Bin]'s Articles
[Yuan, Xiu-Ping]'s Articles
[Li, Ren-Hui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 09-Gao et al-Aquat Microb Ecol-Isolation of a novel cyanophage.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.