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The first study on the effects of microcystin-RR on gene expression profiles of antioxidant enzymes and heat shock protein-70 in Synechocystis sp PCC6803
Li, Huiying; Xie, Ping; Zhang, Dawen; Chen, Jun; Xie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009-05-01
Source PublicationTOXICON
ISSN0041-0101
Volume53Issue:6Pages:595-601
AbstractMicrocystins are heptapeptide toxins produced by cyanobacteria. Microcystin-RR(MC-RR) is a common variant among the 80 variants identified so far. There have been many investigations documenting the toxic effects of microcystins on animals and higher plants, but little is known on the toxic effects of microcystins on algae, especially at molecular level. We studied the effects of MC-RR on gene expression profile of a few antioxidant enzymes and heat shock protein-70 (Hsp70) in Synechocystis sp. PCC6803. After two days post-exposure, a high dose toxin (5 mg/l, about 4.8 x 10(-3) mM) significantly increased expression levels of the genes gpx1, sodB, katG, acnB, gamma-TMTand dnaK2, while a relatively low dose toxin (1 mg/l, about 9.63 x 10(-4) mM) induced a moderate and slow increase of gene expression. Our results indicate that MC-RR could induce the oxidative stress in Synechocystis sp. PCC6803 and the increase in gene expression of antioxidant enzymes and Hsp70 might protect the organism from the oxidative damage. in addition, cell aggregation was observed during the early period of exposure, which might be a specific oxidative stress reaction to MC-RR. (C) 2008 Elsevier Ltd. All rights reserved.; Microcystins are heptapeptide toxins produced by cyanobacteria. Microcystin-RR(MC-RR) is a common variant among the 80 variants identified so far. There have been many investigations documenting the toxic effects of microcystins on animals and higher plants, but little is known on the toxic effects of microcystins on algae, especially at molecular level. We studied the effects of MC-RR on gene expression profile of a few antioxidant enzymes and heat shock protein-70 (Hsp70) in Synechocystis sp. PCC6803. After two days post-exposure, a high dose toxin (5 mg/l, about 4.8 x 10(-3) mM) significantly increased expression levels of the genes gpx1, sodB, katG, acnB, gamma-TMTand dnaK2, while a relatively low dose toxin (1 mg/l, about 9.63 x 10(-4) mM) induced a moderate and slow increase of gene expression. Our results indicate that MC-RR could induce the oxidative stress in Synechocystis sp. PCC6803 and the increase in gene expression of antioxidant enzymes and Hsp70 might protect the organism from the oxidative damage. in addition, cell aggregation was observed during the early period of exposure, which might be a specific oxidative stress reaction to MC-RR. (C) 2008 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMicrocystin-rr Mrna Expression Antioxidant Enzymes Synechocystis Sp Pcc6803 Cell Aggregation
Department[Li, Huiying; Xie, Ping; Zhang, Dawen; Chen, Jun] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaPharmacology & Pharmacy ; Toxicology
DOI10.1016/j.toxicon.2008.11.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101]
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000265358600001
WOS KeywordOXIDATIVE STRESS ; SYNECHOCOCCUS-ELONGATUS ; SP PCC-6803 ; LR ; LAKE ; CYANOBACTERIUM ; GLUTATHIONE ; SYSTEM ; DETOXIFICATION ; AERUGINOSA
Funding OrganizationNational Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101]
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7766
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Huiying,Xie, Ping,Zhang, Dawen,et al. The first study on the effects of microcystin-RR on gene expression profiles of antioxidant enzymes and heat shock protein-70 in Synechocystis sp PCC6803[J]. TOXICON,2009,53(6):595-601.
APA Li, Huiying,Xie, Ping,Zhang, Dawen,Chen, Jun,&Xie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).The first study on the effects of microcystin-RR on gene expression profiles of antioxidant enzymes and heat shock protein-70 in Synechocystis sp PCC6803.TOXICON,53(6),595-601.
MLA Li, Huiying,et al."The first study on the effects of microcystin-RR on gene expression profiles of antioxidant enzymes and heat shock protein-70 in Synechocystis sp PCC6803".TOXICON 53.6(2009):595-601.
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