| The first study on the effects of microcystin-RR on gene expression profiles of antioxidant enzymes and heat shock protein-70 in Synechocystis sp PCC6803 | |
| Li, Huiying; Xie, Ping; Zhang, Dawen; Chen, Jun; Xie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China | |
| 2009-05-01 | |
| Source Publication | TOXICON
 |
| ISSN | 0041-0101 |
| Volume | 53Issue:6Pages:595-601 |
| Abstract | Microcystins are heptapeptide toxins produced by cyanobacteria. Microcystin-RR(MC-RR) is a common variant among the 80 variants identified so far. There have been many investigations documenting the toxic effects of microcystins on animals and higher plants, but little is known on the toxic effects of microcystins on algae, especially at molecular level. We studied the effects of MC-RR on gene expression profile of a few antioxidant enzymes and heat shock protein-70 (Hsp70) in Synechocystis sp. PCC6803. After two days post-exposure, a high dose toxin (5 mg/l, about 4.8 x 10(-3) mM) significantly increased expression levels of the genes gpx1, sodB, katG, acnB, gamma-TMTand dnaK2, while a relatively low dose toxin (1 mg/l, about 9.63 x 10(-4) mM) induced a moderate and slow increase of gene expression. Our results indicate that MC-RR could induce the oxidative stress in Synechocystis sp. PCC6803 and the increase in gene expression of antioxidant enzymes and Hsp70 might protect the organism from the oxidative damage. in addition, cell aggregation was observed during the early period of exposure, which might be a specific oxidative stress reaction to MC-RR. (C) 2008 Elsevier Ltd. All rights reserved.; Microcystins are heptapeptide toxins produced by cyanobacteria. Microcystin-RR(MC-RR) is a common variant among the 80 variants identified so far. There have been many investigations documenting the toxic effects of microcystins on animals and higher plants, but little is known on the toxic effects of microcystins on algae, especially at molecular level. We studied the effects of MC-RR on gene expression profile of a few antioxidant enzymes and heat shock protein-70 (Hsp70) in Synechocystis sp. PCC6803. After two days post-exposure, a high dose toxin (5 mg/l, about 4.8 x 10(-3) mM) significantly increased expression levels of the genes gpx1, sodB, katG, acnB, gamma-TMTand dnaK2, while a relatively low dose toxin (1 mg/l, about 9.63 x 10(-4) mM) induced a moderate and slow increase of gene expression. Our results indicate that MC-RR could induce the oxidative stress in Synechocystis sp. PCC6803 and the increase in gene expression of antioxidant enzymes and Hsp70 might protect the organism from the oxidative damage. in addition, cell aggregation was observed during the early period of exposure, which might be a specific oxidative stress reaction to MC-RR. (C) 2008 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Microcystin-rr Mrna Expression Antioxidant Enzymes Synechocystis Sp Pcc6803 Cell Aggregation |
| Department | [Li, Huiying; Xie, Ping; Zhang, Dawen; Chen, Jun] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Subject Area | Pharmacology & Pharmacy ; Toxicology |
| DOI | 10.1016/j.toxicon.2008.11.005 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Pharmacology & Pharmacy ; Toxicology |
| WOS Subject | Pharmacology & Pharmacy ; Toxicology |
| WOS ID | WOS:000265358600001 |
| WOS Keyword | OXIDATIVE STRESS ; SYNECHOCOCCUS-ELONGATUS ; SP PCC-6803 ; LR ; LAKE ; CYANOBACTERIUM ; GLUTATHIONE ; SYSTEM ; DETOXIFICATION ; AERUGINOSA |
| Funding Organization | National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7766 |
| Collection | 期刊论文 |
| Corresponding Author | Xie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Huiying,Xie, Ping,Zhang, Dawen,et al. The first study on the effects of microcystin-RR on gene expression profiles of antioxidant enzymes and heat shock protein-70 in Synechocystis sp PCC6803[J]. TOXICON,2009,53(6):595-601. |
| APA | Li, Huiying,Xie, Ping,Zhang, Dawen,Chen, Jun,&Xie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).The first study on the effects of microcystin-RR on gene expression profiles of antioxidant enzymes and heat shock protein-70 in Synechocystis sp PCC6803.TOXICON,53(6),595-601. |
| MLA | Li, Huiying,et al."The first study on the effects of microcystin-RR on gene expression profiles of antioxidant enzymes and heat shock protein-70 in Synechocystis sp PCC6803".TOXICON 53.6(2009):595-601. |
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