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Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene
Zhou, Xiuxia1,2; Guo, Qionglin1; Dai, Heping1; Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume33Issue:7Pages:838-847
AbstractIn this study, an IL-8 homologue has been cloned and identified from a reptile, Chinese soft-shelled turtle for the first time. The full-length cDNA of turtle IL-8 was 1188 bp and contained a 312 bp open reading frame (ORF) coding for a protein of 104 amino acids. The chemokine CXC domain, which contained Glu-Leu-Arg (ELR) motif and four cysteine residues, was well conserved in turtle IL-8. The 4924 bp genomic DNA of turtle IL-8 contained four exons and three introns. Phylogenetic analysis showed that the amino acid sequence of turtle IL-8 clustered together with birds. RT-PCR analysis showed that turtle IL-8 mRNA was constitutively expressed liver, spleen, kidney, heart, blood and intestine tissues of control turtles. Real-time quantitative PCR analysis further indicated that the turtle IL-8 mRNA expression was apparent in various tissues at 8 h and up-regulated significantly during 8 h-7 d after Aeromonas hydrophila infection. The present studies will help us to understand the evolution of IL-8 molecule and the inflammatory response mechanism in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.; In this study, an IL-8 homologue has been cloned and identified from a reptile, Chinese soft-shelled turtle for the first time. The full-length cDNA of turtle IL-8 was 1188 bp and contained a 312 bp open reading frame (ORF) coding for a protein of 104 amino acids. The chemokine CXC domain, which contained Glu-Leu-Arg (ELR) motif and four cysteine residues, was well conserved in turtle IL-8. The 4924 bp genomic DNA of turtle IL-8 contained four exons and three introns. Phylogenetic analysis showed that the amino acid sequence of turtle IL-8 clustered together with birds. RT-PCR analysis showed that turtle IL-8 mRNA was constitutively expressed liver, spleen, kidney, heart, blood and intestine tissues of control turtles. Real-time quantitative PCR analysis further indicated that the turtle IL-8 mRNA expression was apparent in various tissues at 8 h and up-regulated significantly during 8 h-7 d after Aeromonas hydrophila infection. The present studies will help us to understand the evolution of IL-8 molecule and the inflammatory response mechanism in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordInterleukin-8 (Il-8) Chemokine Molecular Cloning Aeromonas Hydrophila Chinese Soft-shelled Turtle
Department[Zhou, Xiuxia; Guo, Qionglin; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Xiuxia] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaImmunology ; Zoology
DOI10.1016/j.dci.2009.02.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000266152600006
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; 3'-UNTRANSLATED REGION ; RECEPTOR-BINDING ; CXC CHEMOKINES ; CELLS ; CLONING ; IDENTIFICATION ; ANGIOGENESIS ; CYTOKINES ; CHICKEN
Funding OrganizationNational Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7738
Collection期刊论文
Corresponding AuthorGuo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Xiuxia,Guo, Qionglin,Dai, Heping,et al. Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(7):838-847.
APA Zhou, Xiuxia,Guo, Qionglin,Dai, Heping,&Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(7),838-847.
MLA Zhou, Xiuxia,et al."Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.7(2009):838-847.
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