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学科主题: Endocrinology & Metabolism
题名: The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development
作者: Li, Xi1, 2; He, Jiangyan1; Hu, Wei1; Yin, Zhan1, 2, 3
通讯作者: Yin, Z, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China
关键词: DES-ACYL GHRELIN ; COMPLEMENTARY DEOXYRIBONUCLEIC-ACID ; FOOD-INTAKE ; SECRETAGOGUE RECEPTOR ; FUNCTIONAL-CHARACTERIZATION ; ENERGY-BALANCE ; GENE ; PITUITARY ; PEPTIDE ; OBESTATIN
刊名: ENDOCRINOLOGY
发表日期: 2009-06-01
DOI: 10.1210/en.2008-1398
卷: 150, 期:6, 页:2767-2774
收录类别: SCI
文章类型: Article
部门归属: [Li, Xi; He, Jiangyan; Hu, Wei; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China; [Li, Xi; Yin, Zhan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Yin, Zhan] Huazhong Univ Sci & Technol, Chinese Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113]
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
摘要: Ghrelin, a multifunctional hormone, including potent GH stimulation activity, has been suggested to be important during embryonic development. Expression of ghrelin has been confirmed in the zebrafish pancreas during embryonic stages. Interfering with ghrelin function using two specific antisense morpholino oligonucleotides causes defects during zebrafish embryonic development. In ghrelin morphants the expression of GH was abolished in zebrafish somatotropes, whereas the expression patterns of the other key molecules involved in hypothalamic-pituitary development and distinct pituitary hormones genes remain largely intact at the appropriate time during zebrafish adenohypophysis development. Effective rescue of the ghrelin morphants with exogenous ghrelin mRNA showed that the correct gene had been targeted. Moreover, by analyzing the efficiencies of the ghrelin morphants rescue experiments with various forms of exogenous mutant ghrelin mRNAs, we also demonstrated the essentiality of the form acyl-ghrelin on GH stimulation during zebrafish adenohypophysis development. Our in vivo experiments, for the first time, also provided evidence of the existence of functional obestatin in the C-terminal part of zebrafish proghrelin peptides. Our research here has demonstrated that zebrafish is a unique model for functional studies of endogenous ghrelin, especially during embryonic development. (Endocrinology 150: 2767-2774, 2009)
英文摘要: Ghrelin, a multifunctional hormone, including potent GH stimulation activity, has been suggested to be important during embryonic development. Expression of ghrelin has been confirmed in the zebrafish pancreas during embryonic stages. Interfering with ghrelin function using two specific antisense morpholino oligonucleotides causes defects during zebrafish embryonic development. In ghrelin morphants the expression of GH was abolished in zebrafish somatotropes, whereas the expression patterns of the other key molecules involved in hypothalamic-pituitary development and distinct pituitary hormones genes remain largely intact at the appropriate time during zebrafish adenohypophysis development. Effective rescue of the ghrelin morphants with exogenous ghrelin mRNA showed that the correct gene had been targeted. Moreover, by analyzing the efficiencies of the ghrelin morphants rescue experiments with various forms of exogenous mutant ghrelin mRNAs, we also demonstrated the essentiality of the form acyl-ghrelin on GH stimulation during zebrafish adenohypophysis development. Our in vivo experiments, for the first time, also provided evidence of the existence of functional obestatin in the C-terminal part of zebrafish proghrelin peptides. Our research here has demonstrated that zebrafish is a unique model for functional studies of endogenous ghrelin, especially during embryonic development. (Endocrinology 150: 2767-2774, 2009)
关键词[WOS]: DES-ACYL GHRELIN ; COMPLEMENTARY DEOXYRIBONUCLEIC-ACID ; FOOD-INTAKE ; SECRETAGOGUE RECEPTOR ; FUNCTIONAL-CHARACTERIZATION ; ENERGY-BALANCE ; GENE ; PITUITARY ; PEPTIDE ; OBESTATIN
语种: 英语
WOS记录号: WOS:000266256700038
ISSN号: 0013-7227
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7736
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Huazhong Univ Sci & Technol, Chinese Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Hubei, Peoples R China

Recommended Citation:
Li, Xi; He, Jiangyan; Hu, Wei; Yin, Zhan.The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development,ENDOCRINOLOGY,2009,150(6):2767-2774
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