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The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development
Li, Xi1,2; He, Jiangyan1; Hu, Wei1; Yin, Zhan1,2,3; Yin, Z, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China
2009-06-01
Source PublicationENDOCRINOLOGY
ISSN0013-7227
Volume150Issue:6Pages:2767-2774
AbstractGhrelin, a multifunctional hormone, including potent GH stimulation activity, has been suggested to be important during embryonic development. Expression of ghrelin has been confirmed in the zebrafish pancreas during embryonic stages. Interfering with ghrelin function using two specific antisense morpholino oligonucleotides causes defects during zebrafish embryonic development. In ghrelin morphants the expression of GH was abolished in zebrafish somatotropes, whereas the expression patterns of the other key molecules involved in hypothalamic-pituitary development and distinct pituitary hormones genes remain largely intact at the appropriate time during zebrafish adenohypophysis development. Effective rescue of the ghrelin morphants with exogenous ghrelin mRNA showed that the correct gene had been targeted. Moreover, by analyzing the efficiencies of the ghrelin morphants rescue experiments with various forms of exogenous mutant ghrelin mRNAs, we also demonstrated the essentiality of the form acyl-ghrelin on GH stimulation during zebrafish adenohypophysis development. Our in vivo experiments, for the first time, also provided evidence of the existence of functional obestatin in the C-terminal part of zebrafish proghrelin peptides. Our research here has demonstrated that zebrafish is a unique model for functional studies of endogenous ghrelin, especially during embryonic development. (Endocrinology 150: 2767-2774, 2009); Ghrelin, a multifunctional hormone, including potent GH stimulation activity, has been suggested to be important during embryonic development. Expression of ghrelin has been confirmed in the zebrafish pancreas during embryonic stages. Interfering with ghrelin function using two specific antisense morpholino oligonucleotides causes defects during zebrafish embryonic development. In ghrelin morphants the expression of GH was abolished in zebrafish somatotropes, whereas the expression patterns of the other key molecules involved in hypothalamic-pituitary development and distinct pituitary hormones genes remain largely intact at the appropriate time during zebrafish adenohypophysis development. Effective rescue of the ghrelin morphants with exogenous ghrelin mRNA showed that the correct gene had been targeted. Moreover, by analyzing the efficiencies of the ghrelin morphants rescue experiments with various forms of exogenous mutant ghrelin mRNAs, we also demonstrated the essentiality of the form acyl-ghrelin on GH stimulation during zebrafish adenohypophysis development. Our in vivo experiments, for the first time, also provided evidence of the existence of functional obestatin in the C-terminal part of zebrafish proghrelin peptides. Our research here has demonstrated that zebrafish is a unique model for functional studies of endogenous ghrelin, especially during embryonic development. (Endocrinology 150: 2767-2774, 2009)
SubtypeArticle
KeywordDes-acyl Ghrelin Complementary Deoxyribonucleic-acid Food-intake Secretagogue Receptor Functional-characterization Energy-balance Gene Pituitary Peptide Obestatin
Department[Li, Xi; He, Jiangyan; Hu, Wei; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China; [Li, Xi; Yin, Zhan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Yin, Zhan] Huazhong Univ Sci & Technol, Chinese Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Hubei, Peoples R China
Subject AreaEndocrinology & Metabolism
DOI10.1210/en.2008-1398
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113] ; National Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113] ; National Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113] ; National Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113]
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000266256700038
WOS KeywordDES-ACYL GHRELIN ; COMPLEMENTARY DEOXYRIBONUCLEIC-ACID ; FOOD-INTAKE ; SECRETAGOGUE RECEPTOR ; FUNCTIONAL-CHARACTERIZATION ; ENERGY-BALANCE ; GENE ; PITUITARY ; PEPTIDE ; OBESTATIN
Funding OrganizationNational Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113] ; National Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113] ; National Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113] ; National Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7736
Collection期刊论文
Corresponding AuthorYin, Z, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Huazhong Univ Sci & Technol, Chinese Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Li, Xi,He, Jiangyan,Hu, Wei,et al. The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development[J]. ENDOCRINOLOGY,2009,150(6):2767-2774.
APA Li, Xi,He, Jiangyan,Hu, Wei,Yin, Zhan,&Yin, Z, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China.(2009).The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development.ENDOCRINOLOGY,150(6),2767-2774.
MLA Li, Xi,et al."The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development".ENDOCRINOLOGY 150.6(2009):2767-2774.
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