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学科主题: Microbiology; Veterinary Sciences
题名: First description of a novel Weissella species as an opportunistic pathogen for rainbow trout Oncorhynchus mykiss (Walbaum) in China
作者: Liu, Jin Yu1, 2; Li, Ai Hua1; Ji, Cheng1, 2; Yang, Wu Ming1, 2
通讯作者: Li, AH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: Weissella ; Identification ; Description ; Rainbow trout
刊名: VETERINARY MICROBIOLOGY
发表日期: 2009-05-12
DOI: 10.1016/j.vetmic.2008.11.027
卷: 136, 期:3-4, 页:314-320
收录类别: SCI
文章类型: Article
部门归属: [Liu, Jin Yu; Li, Ai Hua; Ji, Cheng; Yang, Wu Ming] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, Jin Yu; Ji, Cheng; Yang, Wu Ming] Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112]
类目[WOS]: Microbiology ; Veterinary Sciences
研究领域[WOS]: Microbiology ; Veterinary Sciences
摘要: Six strains of Gram-positive, catalase-negative, non-motile, irregular short rod-shaped Weissella bacteria, with width and length of 0.5-0.6 and 1.2-2.7 mu m were isolated from diseased rainbow trout Oncorhynchus mykiss (Walbaum) in winter of 2007 at a commercial fishery in Jingmen, Hubei province, China. The diseased rainbow trout exhibited hemorrhage in eyes, anal region, intestine and abdomen wall, petechia of liver, some fish with hydrocele in stomach. Six isolates had identical biochemical reactions, phylogenetic analysis of 16S rDNA sequences, amplified ribosomal DNA restriction analysis (ARDRA), enzymatic profile analysis and antimicrobial susceptibility results, indicating as a single clonal outbreak. But all were different from any other validated twelve Weissella species in the term of physiological and biochemical characters. It is indicated that isolates are phylogenetically closer to Weissella halotolerans, Weissella viridescens and Weissella minor on 16S rDNA phylogenetic analysis result, than to W halotolerans and W viridescens on the result of ARDRA study and enzymatic profile analysis. Antimicrobial susceptibility testing was used to scan effective drugs for the therapy of this disease. Experimental infection assays with one isolate were conducted and pathogenicity (by intraperitoneal injection) was demonstrated in rainbow trout O. mykiss (Walbaum) and crucian carp (Carassius auratus gibelio) fingerlings. Because no Weissella was detected in fish feedstuffs and pond water, the source of this pathogen remains unknown, and Weissella isolates were regarded as an opportunistic pathogen for rainbow trout. This is the first report of Weissella strains which can cause disease of cultured fish in the world. (C) 2009 Elsevier B.V. All rights reserved.
英文摘要: Six strains of Gram-positive, catalase-negative, non-motile, irregular short rod-shaped Weissella bacteria, with width and length of 0.5-0.6 and 1.2-2.7 mu m were isolated from diseased rainbow trout Oncorhynchus mykiss (Walbaum) in winter of 2007 at a commercial fishery in Jingmen, Hubei province, China. The diseased rainbow trout exhibited hemorrhage in eyes, anal region, intestine and abdomen wall, petechia of liver, some fish with hydrocele in stomach. Six isolates had identical biochemical reactions, phylogenetic analysis of 16S rDNA sequences, amplified ribosomal DNA restriction analysis (ARDRA), enzymatic profile analysis and antimicrobial susceptibility results, indicating as a single clonal outbreak. But all were different from any other validated twelve Weissella species in the term of physiological and biochemical characters. It is indicated that isolates are phylogenetically closer to Weissella halotolerans, Weissella viridescens and Weissella minor on 16S rDNA phylogenetic analysis result, than to W halotolerans and W viridescens on the result of ARDRA study and enzymatic profile analysis. Antimicrobial susceptibility testing was used to scan effective drugs for the therapy of this disease. Experimental infection assays with one isolate were conducted and pathogenicity (by intraperitoneal injection) was demonstrated in rainbow trout O. mykiss (Walbaum) and crucian carp (Carassius auratus gibelio) fingerlings. Because no Weissella was detected in fish feedstuffs and pond water, the source of this pathogen remains unknown, and Weissella isolates were regarded as an opportunistic pathogen for rainbow trout. This is the first report of Weissella strains which can cause disease of cultured fish in the world. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: LACTIC-ACID BACTERIA ; GRAM-POSITIVE COCCI ; SP-NOV. ; IDENTIFICATION ; LACTOBACILLUS ; CONFUSA ; LEUCONOSTOC ; KIMCHI ; FISH ; FOOD
语种: 英语
WOS记录号: WOS:000266118800015
ISSN号: 0378-1135
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7734
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Liu, Jin Yu; Li, Ai Hua; Ji, Cheng; Yang, Wu Ming.First description of a novel Weissella species as an opportunistic pathogen for rainbow trout Oncorhynchus mykiss (Walbaum) in China,VETERINARY MICROBIOLOGY,2009,136(3-4):314-320
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