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First description of a novel Weissella species as an opportunistic pathogen for rainbow trout Oncorhynchus mykiss (Walbaum) in China
Liu, Jin Yu1,2; Li, Ai Hua1; Ji, Cheng1,2; Yang, Wu Ming1,2; Li, AH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009-05-12
Source PublicationVETERINARY MICROBIOLOGY
ISSN0378-1135
Volume136Issue:3-4Pages:314-320
AbstractSix strains of Gram-positive, catalase-negative, non-motile, irregular short rod-shaped Weissella bacteria, with width and length of 0.5-0.6 and 1.2-2.7 mu m were isolated from diseased rainbow trout Oncorhynchus mykiss (Walbaum) in winter of 2007 at a commercial fishery in Jingmen, Hubei province, China. The diseased rainbow trout exhibited hemorrhage in eyes, anal region, intestine and abdomen wall, petechia of liver, some fish with hydrocele in stomach. Six isolates had identical biochemical reactions, phylogenetic analysis of 16S rDNA sequences, amplified ribosomal DNA restriction analysis (ARDRA), enzymatic profile analysis and antimicrobial susceptibility results, indicating as a single clonal outbreak. But all were different from any other validated twelve Weissella species in the term of physiological and biochemical characters. It is indicated that isolates are phylogenetically closer to Weissella halotolerans, Weissella viridescens and Weissella minor on 16S rDNA phylogenetic analysis result, than to W halotolerans and W viridescens on the result of ARDRA study and enzymatic profile analysis. Antimicrobial susceptibility testing was used to scan effective drugs for the therapy of this disease. Experimental infection assays with one isolate were conducted and pathogenicity (by intraperitoneal injection) was demonstrated in rainbow trout O. mykiss (Walbaum) and crucian carp (Carassius auratus gibelio) fingerlings. Because no Weissella was detected in fish feedstuffs and pond water, the source of this pathogen remains unknown, and Weissella isolates were regarded as an opportunistic pathogen for rainbow trout. This is the first report of Weissella strains which can cause disease of cultured fish in the world. (C) 2009 Elsevier B.V. All rights reserved.; Six strains of Gram-positive, catalase-negative, non-motile, irregular short rod-shaped Weissella bacteria, with width and length of 0.5-0.6 and 1.2-2.7 mu m were isolated from diseased rainbow trout Oncorhynchus mykiss (Walbaum) in winter of 2007 at a commercial fishery in Jingmen, Hubei province, China. The diseased rainbow trout exhibited hemorrhage in eyes, anal region, intestine and abdomen wall, petechia of liver, some fish with hydrocele in stomach. Six isolates had identical biochemical reactions, phylogenetic analysis of 16S rDNA sequences, amplified ribosomal DNA restriction analysis (ARDRA), enzymatic profile analysis and antimicrobial susceptibility results, indicating as a single clonal outbreak. But all were different from any other validated twelve Weissella species in the term of physiological and biochemical characters. It is indicated that isolates are phylogenetically closer to Weissella halotolerans, Weissella viridescens and Weissella minor on 16S rDNA phylogenetic analysis result, than to W halotolerans and W viridescens on the result of ARDRA study and enzymatic profile analysis. Antimicrobial susceptibility testing was used to scan effective drugs for the therapy of this disease. Experimental infection assays with one isolate were conducted and pathogenicity (by intraperitoneal injection) was demonstrated in rainbow trout O. mykiss (Walbaum) and crucian carp (Carassius auratus gibelio) fingerlings. Because no Weissella was detected in fish feedstuffs and pond water, the source of this pathogen remains unknown, and Weissella isolates were regarded as an opportunistic pathogen for rainbow trout. This is the first report of Weissella strains which can cause disease of cultured fish in the world. (C) 2009 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordWeissella Identification Description Rainbow Trout
Department[Liu, Jin Yu; Li, Ai Hua; Ji, Cheng; Yang, Wu Ming] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, Jin Yu; Ji, Cheng; Yang, Wu Ming] Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Subject AreaMicrobiology ; Veterinary Sciences
DOI10.1016/j.vetmic.2008.11.027
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112] ; National Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112] ; National Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112] ; National Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology ; Veterinary Sciences
WOS SubjectMicrobiology ; Veterinary Sciences
WOS IDWOS:000266118800015
WOS KeywordLACTIC-ACID BACTERIA ; GRAM-POSITIVE COCCI ; SP-NOV. ; IDENTIFICATION ; LACTOBACILLUS ; CONFUSA ; LEUCONOSTOC ; KIMCHI ; FISH ; FOOD
Funding OrganizationNational Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112] ; National Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112] ; National Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112] ; National Science & Technology Pillar Programs [2006BAD03B04, 2006BAK02A22]; National Natural Science Foundation of China [30670112]
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Cited Times:56[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7734
Collection期刊论文
Corresponding AuthorLi, AH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jin Yu,Li, Ai Hua,Ji, Cheng,et al. First description of a novel Weissella species as an opportunistic pathogen for rainbow trout Oncorhynchus mykiss (Walbaum) in China[J]. VETERINARY MICROBIOLOGY,2009,136(3-4):314-320.
APA Liu, Jin Yu,Li, Ai Hua,Ji, Cheng,Yang, Wu Ming,&Li, AH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).First description of a novel Weissella species as an opportunistic pathogen for rainbow trout Oncorhynchus mykiss (Walbaum) in China.VETERINARY MICROBIOLOGY,136(3-4),314-320.
MLA Liu, Jin Yu,et al."First description of a novel Weissella species as an opportunistic pathogen for rainbow trout Oncorhynchus mykiss (Walbaum) in China".VETERINARY MICROBIOLOGY 136.3-4(2009):314-320.
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