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学科主题: Mycology
题名: Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish
作者: Ke, Xiao L.1, 2; Wang, Jian G.1; Gu, Ze M.3; Li, Ming4; Gong, Xiao N.1
通讯作者: Wang, JG, Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China
关键词: Fish disease ; ITS rDNA ; Molecular phylogeny ; Saprolegnia ; Systematic
刊名: MYCOLOGICAL RESEARCH
发表日期: 2009-05-01
DOI: 10.1016/j.mycres.2009.01.008
卷: 113, 页:637-644
收录类别: SCI
文章类型: Article
部门归属: [Ke, Xiao L.; Wang, Jian G.; Gong, Xiao N.] Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China; [Ke, Xiao L.] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Gu, Ze M.] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China; [Li, Ming] Wuhan Polytech Univ, Feed Sci Dept, Wuhan 430023, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Chinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700]
类目[WOS]: Mycology
研究领域[WOS]: Mycology
摘要: Two Saprolegnia isolates, JY isolated from silver crucian carp (Carassius auratus gibelio Bloch) and BMY isolated from zebra fish (Brachydanio rerio Hamilton) came from infections occurring concurrently in different locations in China. To confirm whether the two isolates were from the same Saprolegnia clone, comparative studies have been carried out based on their morphological, physiological and molecular characteristics. Observations showed that morphologically (both asexual and sexual organs) the two isolates were broadly similar and both isolates under-went repeated zoospore emergence. Comparing 704 base pairs of internal transcribed spacer (ITS) region and the 5.8S rDNA, we found isolates JY and BMY shared an identical ITS sequence with a minor variation (99.6 % similarity). Forty available sequences for representatives Saprolegnia spp. belonged to four phylogenetically separate clades. The two studied isolates fell within clade I that comprised a group of isolates which showed almost an identical ITS sequence but had been identified as a number of different morphological species. our findings suggest that isolates JY and BMY appear to belong to the S. ferax clade and this clade (1) contains a number of closely related phylogenetic species. This is distinct from the more common fish pathogenic isolates, which belong to the S. parasitica clade (III) and are characterized by having cysts decorated by bundles of long hooked hairs and two further clades (II and IV) containing largely saprotrophic or soil born species. (C) 2009 Published by Elsevier Ltd on behalf of The British Mycological Society.
英文摘要: Two Saprolegnia isolates, JY isolated from silver crucian carp (Carassius auratus gibelio Bloch) and BMY isolated from zebra fish (Brachydanio rerio Hamilton) came from infections occurring concurrently in different locations in China. To confirm whether the two isolates were from the same Saprolegnia clone, comparative studies have been carried out based on their morphological, physiological and molecular characteristics. Observations showed that morphologically (both asexual and sexual organs) the two isolates were broadly similar and both isolates under-went repeated zoospore emergence. Comparing 704 base pairs of internal transcribed spacer (ITS) region and the 5.8S rDNA, we found isolates JY and BMY shared an identical ITS sequence with a minor variation (99.6 % similarity). Forty available sequences for representatives Saprolegnia spp. belonged to four phylogenetically separate clades. The two studied isolates fell within clade I that comprised a group of isolates which showed almost an identical ITS sequence but had been identified as a number of different morphological species. our findings suggest that isolates JY and BMY appear to belong to the S. ferax clade and this clade (1) contains a number of closely related phylogenetic species. This is distinct from the more common fish pathogenic isolates, which belong to the S. parasitica clade (III) and are characterized by having cysts decorated by bundles of long hooked hairs and two further clades (II and IV) containing largely saprotrophic or soil born species. (C) 2009 Published by Elsevier Ltd on behalf of The British Mycological Society.
关键词[WOS]: SEQUENCE ALIGNMENT ; BROWN TROUT ; PARASITICA ; FUNGUS ; AMPHIBIANS ; TAXONOMY ; DICLINA ; EGGS
语种: 英语
WOS记录号: WOS:000266473900012
ISSN号: 0953-7562
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7728
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
4.Wuhan Polytech Univ, Feed Sci Dept, Wuhan 430023, Peoples R China

Recommended Citation:
Ke, Xiao L.; Wang, Jian G.; Gu, Ze M.; Li, Ming; Gong, Xiao N..Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish,MYCOLOGICAL RESEARCH,2009,113():637-644
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