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Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish
Ke, Xiao L.; Wang, Jian G.; Gu, Ze M.; Li, Ming; Gong, Xiao N.; Wang, JG, Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China
2009
Source PublicationMYCOLOGICAL RESEARCH
Volume113Pages:637-644
AbstractTwo Saprolegnia isolates, JY isolated from silver crucian carp (Carassius auratus gibelio Bloch) and BMY isolated from zebra fish (Brachydanio rerio Hamilton) came from infections occurring concurrently in different locations in China. To confirm whether the two isolates were from the same Saprolegnia clone, comparative studies have been carried out based on their morphological, physiological and molecular characteristics. Observations showed that morphologically (both asexual and sexual organs) the two isolates were broadly similar and both isolates under-went repeated zoospore emergence. Comparing 704 base pairs of internal transcribed spacer (ITS) region and the 5.8S rDNA, we found isolates JY and BMY shared an identical ITS sequence with a minor variation (99.6 % similarity). Forty available sequences for representatives Saprolegnia spp. belonged to four phylogenetically separate clades. The two studied isolates fell within clade I that comprised a group of isolates which showed almost an identical ITS sequence but had been identified as a number of different morphological species. our findings suggest that isolates JY and BMY appear to belong to the S. ferax clade and this clade (1) contains a number of closely related phylogenetic species. This is distinct from the more common fish pathogenic isolates, which belong to the S. parasitica clade (III) and are characterized by having cysts decorated by bundles of long hooked hairs and two further clades (II and IV) containing largely saprotrophic or soil born species. (C) 2009 Published by Elsevier Ltd on behalf of The British Mycological Society.
KeywordFish Disease Its Rdna Molecular Phylogeny Saprolegnia Systematic
Department[Ke, Xiao L.; Wang, Jian G.; Gong, Xiao N.] Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China; [Ke, Xiao L.] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Gu, Ze M.] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China; [Li, Ming] Wuhan Polytech Univ, Feed Sci Dept, Wuhan 430023, Peoples R China
Subject AreaMycology
Funding OrganizationChinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700] ; Chinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700] ; Chinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700] ; Chinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700]
Indexed BySCI
Language英语
WOS IDWOS:000266473900012
Funding OrganizationChinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700] ; Chinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700] ; Chinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700] ; Chinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700]
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Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7728
Collection期刊论文
Corresponding AuthorWang, JG, Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Ke, Xiao L.,Wang, Jian G.,Gu, Ze M.,et al. Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish[J]. MYCOLOGICAL RESEARCH,2009,113:637-644.
APA Ke, Xiao L.,Wang, Jian G.,Gu, Ze M.,Li, Ming,Gong, Xiao N.,&Wang, JG, Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China.(2009).Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish.MYCOLOGICAL RESEARCH,113,637-644.
MLA Ke, Xiao L.,et al."Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish".MYCOLOGICAL RESEARCH 113(2009):637-644.
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