| Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp | |
| Liu, Jing-Xia; Zhai, Yan-Hua; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China | |
| 2009-07-01 | |
| Source Publication | MOLECULAR BIOLOGY REPORTS
 |
| ISSN | 0301-4851 |
| Volume | 36Issue:6Pages:1491-1496 |
| Abstract | By differential screening, we cloned the CagCNBP, demonstrated its predominant expression in ovary and testis, and reported its development behavior during folliculogenesis and oogenesis by immunofluorescence localization (Liu and Gui, Gene 365:181-192, 2005), but its developmental behavior during spermatogenesis and its transcript distribution during embryogenesis are not revealed. In the present study, by in situ hybridization, we analyze CagCNBP expression pattern during gibel carp embryogenesis. The CagCNBP transcripts ubiquitously distributed in all embryonic cells in early developmental stage embryos, and peak in midbrain, hindbrain and somites of gibel carp larva during organogenesis. By antibody detection, we reveal CagCNBP protein distribution change during spermatogenesis. The cell-specific distribution of CagCNBP is revealed by immunofluorescence staining, and predominant CagCNBP expression in testis somatic cells and spermatogonia is demonstrated in this paper. For the first time, the CNBP distribution during spermatogenesis in vertebrate has been revealed.; By differential screening, we cloned the CagCNBP, demonstrated its predominant expression in ovary and testis, and reported its development behavior during folliculogenesis and oogenesis by immunofluorescence localization (Liu and Gui, Gene 365:181-192, 2005), but its developmental behavior during spermatogenesis and its transcript distribution during embryogenesis are not revealed. In the present study, by in situ hybridization, we analyze CagCNBP expression pattern during gibel carp embryogenesis. The CagCNBP transcripts ubiquitously distributed in all embryonic cells in early developmental stage embryos, and peak in midbrain, hindbrain and somites of gibel carp larva during organogenesis. By antibody detection, we reveal CagCNBP protein distribution change during spermatogenesis. The cell-specific distribution of CagCNBP is revealed by immunofluorescence staining, and predominant CagCNBP expression in testis somatic cells and spermatogonia is demonstrated in this paper. For the first time, the CNBP distribution during spermatogenesis in vertebrate has been revealed. |
| Subtype | Article |
| Keyword | Cnbp Embryogenesis Organogenesis Spermatogenesis Gibel Carp |
| Department | [Liu, Jing-Xia; Zhai, Yan-Hua; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| Subject Area | Biochemistry & Molecular Biology |
| DOI | 10.1007/s11033-008-9340-2 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemistry & Molecular Biology |
| WOS ID | WOS:000266454500039 |
| WOS Keyword | ZINC-FINGER PROTEIN ; CARASSIUS-AURATUS-GIBELIO ; DEVELOPMENTAL EXPRESSION ; MOUSE ; GENE ; OOGENESIS ; BEHAVIOR ; FISH |
| Funding Organization | National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7714 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Jing-Xia,Zhai, Yan-Hua,Gui, Jian-Fang,et al. Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1491-1496. |
| APA | Liu, Jing-Xia,Zhai, Yan-Hua,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China.(2009).Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp.MOLECULAR BIOLOGY REPORTS,36(6),1491-1496. |
| MLA | Liu, Jing-Xia,et al."Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp".MOLECULAR BIOLOGY REPORTS 36.6(2009):1491-1496. |
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