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Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp
Liu, Jing-Xia; Zhai, Yan-Hua; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
2009-07-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume36Issue:6Pages:1491-1496
AbstractBy differential screening, we cloned the CagCNBP, demonstrated its predominant expression in ovary and testis, and reported its development behavior during folliculogenesis and oogenesis by immunofluorescence localization (Liu and Gui, Gene 365:181-192, 2005), but its developmental behavior during spermatogenesis and its transcript distribution during embryogenesis are not revealed. In the present study, by in situ hybridization, we analyze CagCNBP expression pattern during gibel carp embryogenesis. The CagCNBP transcripts ubiquitously distributed in all embryonic cells in early developmental stage embryos, and peak in midbrain, hindbrain and somites of gibel carp larva during organogenesis. By antibody detection, we reveal CagCNBP protein distribution change during spermatogenesis. The cell-specific distribution of CagCNBP is revealed by immunofluorescence staining, and predominant CagCNBP expression in testis somatic cells and spermatogonia is demonstrated in this paper. For the first time, the CNBP distribution during spermatogenesis in vertebrate has been revealed.; By differential screening, we cloned the CagCNBP, demonstrated its predominant expression in ovary and testis, and reported its development behavior during folliculogenesis and oogenesis by immunofluorescence localization (Liu and Gui, Gene 365:181-192, 2005), but its developmental behavior during spermatogenesis and its transcript distribution during embryogenesis are not revealed. In the present study, by in situ hybridization, we analyze CagCNBP expression pattern during gibel carp embryogenesis. The CagCNBP transcripts ubiquitously distributed in all embryonic cells in early developmental stage embryos, and peak in midbrain, hindbrain and somites of gibel carp larva during organogenesis. By antibody detection, we reveal CagCNBP protein distribution change during spermatogenesis. The cell-specific distribution of CagCNBP is revealed by immunofluorescence staining, and predominant CagCNBP expression in testis somatic cells and spermatogonia is demonstrated in this paper. For the first time, the CNBP distribution during spermatogenesis in vertebrate has been revealed.
SubtypeArticle
KeywordCnbp Embryogenesis Organogenesis Spermatogenesis Gibel Carp
Department[Liu, Jing-Xia; Zhai, Yan-Hua; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology
DOI10.1007/s11033-008-9340-2
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000266454500039
WOS KeywordZINC-FINGER PROTEIN ; CARASSIUS-AURATUS-GIBELIO ; DEVELOPMENTAL EXPRESSION ; MOUSE ; GENE ; OOGENESIS ; BEHAVIOR ; FISH
Funding OrganizationNational Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401] ; National Major Basic Research Program [2004CB117401]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7714
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jing-Xia,Zhai, Yan-Hua,Gui, Jian-Fang,et al. Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1491-1496.
APA Liu, Jing-Xia,Zhai, Yan-Hua,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China.(2009).Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp.MOLECULAR BIOLOGY REPORTS,36(6),1491-1496.
MLA Liu, Jing-Xia,et al."Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp".MOLECULAR BIOLOGY REPORTS 36.6(2009):1491-1496.
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