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Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos
Pei, De-Sheng; Sun, Yong-Hua; Zhu, Zuo-Yan; Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009
Source PublicationMOLECULAR BIOLOGY REPORTS
Volume36Issue:6Pages:1375-1380
AbstractA novel gene-K23, differentially expressed in cross-subfamily cloned embryos, was isolated by RACE-PCR technique. It had 2580 base pairs (bp) in length, with a 1,425 bp open reading frame (ORF) encoding a putative protein of 474 amino acids (aa). Bioinformatic analysis indicated that K23 had 22 phosphorylation sites, but it had no signal peptides. Developmental expression analysis in zebrafish showed that K23 transcripts were maternally expressed in ovum and the amount of K23 transcripts increased gradually from zygote to pharyngula period. Subcellular localization analysis revealed that K23 protein was homogeneously distributed both in nuclei and cytoplasm. Taken together, our findings indicate that K23 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos.
KeywordNuclear Reprogramming Nuclear Transfer Rare Minnow (Gobiocypris Rarus) Zebrafish (Danio Rerio)
Department[Pei, De-Sheng; Sun, Yong-Hua; Zhu, Zuo-Yan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Pei, De-Sheng] Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology
Funding OrganizationNational Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406] ; National Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406] ; National Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406] ; National Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406]
Indexed BySCI
Language英语
WOS IDWOS:000266454500023
Funding OrganizationNational Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406] ; National Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406] ; National Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406] ; National Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7712
Collection期刊论文
Corresponding AuthorSun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Pei, De-Sheng,Sun, Yong-Hua,Zhu, Zuo-Yan,et al. Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1375-1380.
APA Pei, De-Sheng,Sun, Yong-Hua,Zhu, Zuo-Yan,&Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos.MOLECULAR BIOLOGY REPORTS,36(6),1375-1380.
MLA Pei, De-Sheng,et al."Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos".MOLECULAR BIOLOGY REPORTS 36.6(2009):1375-1380.
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