IHB OpenIR  > 期刊论文
Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)
Su, Jianguo1,2; Yang, Chunrong2; Zhu, Zuoyan1; Wang, Yaping1; Jang, Songhun1; Liao, Lanjie1; Wang, YP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009-06-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume26Issue:6Pages:828-835
AbstractIn the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vivo. In the present study, an Mx-like gene was cloned, characterized and gene-transferred in rare minnow Gobiocypris rarus, and its antiviral activity was confirmed in vivo. The full length of the rare minnow Mx-like cDNA is 2241 bp in length and encodes a polypeptide of 625 amino acids with an estimated molecular mass of 70.928 kDa and a predicted isoelectric point of 7.33. Analysis of the deduced amino acid sequence indicated that the mature peptide contains an amino-terminal tripartite GTP-binding motif, a dynamin family signature sequence, a GTPase effector domain and two carboxy-terminal leucine zipper motifs, and is the most similar to the crucian carp (Carassius auratus) Mx3 sequence with an identity of 89%. Both P0 and F1 generations of Mx-transgenic rare minnow demonstrated very significantly high survival rate to GCRV infection (P < 0.01). The mRNA expression of Mx gene was consistent with survival rate in F1 generation. The virus yield was also concurrent with survival time using electron microscope technology. Rare minnow has Mx gene(s) of its own but introducing more Mx gene improves their resistance to GCRV. Mx-transgenic rare minnow might contribute to control the GCRV diseases. (C) 2008 Published by Elsevier Ltd.; In the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vivo. In the present study, an Mx-like gene was cloned, characterized and gene-transferred in rare minnow Gobiocypris rarus, and its antiviral activity was confirmed in vivo. The full length of the rare minnow Mx-like cDNA is 2241 bp in length and encodes a polypeptide of 625 amino acids with an estimated molecular mass of 70.928 kDa and a predicted isoelectric point of 7.33. Analysis of the deduced amino acid sequence indicated that the mature peptide contains an amino-terminal tripartite GTP-binding motif, a dynamin family signature sequence, a GTPase effector domain and two carboxy-terminal leucine zipper motifs, and is the most similar to the crucian carp (Carassius auratus) Mx3 sequence with an identity of 89%. Both P0 and F1 generations of Mx-transgenic rare minnow demonstrated very significantly high survival rate to GCRV infection (P < 0.01). The mRNA expression of Mx gene was consistent with survival rate in F1 generation. The virus yield was also concurrent with survival time using electron microscope technology. Rare minnow has Mx gene(s) of its own but introducing more Mx gene improves their resistance to GCRV. Mx-transgenic rare minnow might contribute to control the GCRV diseases. (C) 2008 Published by Elsevier Ltd.
SubtypeArticle
KeywordRare Minnow (Gobiocypris Rarus) Mx Gene Grass Carp Reovirus Transgenic Fish Antiviral Activity Gene Cloning
Department[Su, Jianguo; Zhu, Zuoyan; Wang, Yaping; Jang, Songhun; Liao, Lanjie] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Su, Jianguo; Yang, Chunrong] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2008.12.007
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09] ; National Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09] ; National Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09] ; National Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000266965900002
WOS KeywordVESICULAR STOMATITIS-VIRUS ; IN-VITRO INHIBITION ; INFLUENZA-VIRUS ; ANTIVIRAL ACTIVITY ; ATLANTIC SALMON ; EXPRESSION ANALYSIS ; SOLE AQUABIRNAVIRUS ; CELL-LINES ; PROTEIN ; GENE
Funding OrganizationNational Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09] ; National Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09] ; National Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09] ; National Natural Science Foundation of China [30740009, 30871917]; project-sponsored by SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]; China Postdoctoral Science Foundation [20070410298]; Institute of Hydrobiology [2007FB09]
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7708
Collection期刊论文
Corresponding AuthorWang, YP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China
Recommended Citation
GB/T 7714
Su, Jianguo,Yang, Chunrong,Zhu, Zuoyan,et al. Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(6):828-835.
APA Su, Jianguo.,Yang, Chunrong.,Zhu, Zuoyan.,Wang, Yaping.,Jang, Songhun.,...&Wang, YP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus).FISH & SHELLFISH IMMUNOLOGY,26(6),828-835.
MLA Su, Jianguo,et al."Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)".FISH & SHELLFISH IMMUNOLOGY 26.6(2009):828-835.
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