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Zebrafish eaf1 and eaf2/u19 Mediate Effective Convergence and Extension Movements through the Maintenance of wnt11 and wnt5 Expression
Liu, Jing-Xia1; Hu, Bo1; Wang, Yang2; Gui, Jian-Fang2; Xiao, Wuhan1; Xiao, WH, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2009-06-12
Source PublicationJOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
Volume284Issue:24Pages:16679-16692
AbstractStudies have attributed several functions to the Eaf family, including tumor suppression and eye development. Given the potential association between cancer and development, we set forth to explore Eaf1 and Eaf2/U19 activity in vertebrate embryogenesis, using zebrafish. In situ hybridization revealed similar eaf1 and eaf2/u19 expression patterns. Morpholino-mediated knockdown of either eaf1 or eaf2/u19 expression produced similar morphological changes that could be reversed by ectopic expression of target or reciprocal-target mRNA. However, combination of Eaf1 and Eaf2/U19 (Eafs)-morpholinos increased the severity of defects, suggesting that Eaf1 and Eaf2/U19 only share some functional redundancy. The Eafs knockdown phenotype resembled that of embryos with defects in convergence and extension movements. Indeed, knockdown caused expression pattern changes for convergence and extension movement markers, whereas cell tracing experiments using kaeda mRNA showed a correlation between Eafs knockdown and cell migration defects. Cardiac and pancreatic differentiation markers revealed that Eafs knockdown also disrupted midline convergence of heart and pancreatic organ precursors. Noncanonical Wnt signaling plays a key role in both convergence and extension movements and midline convergence of organ precursors. We found that Eaf1 and Eaf2/U19 maintained expression levels of wnt11 and wnt5. Moreover, wnt11 or wnt5 mRNA partially rescued the convergence and extension movement defects occurring in eafs morphants. Wnt11 and Wnt5 converge on rhoA, so not surprisingly, rhoA mRNA more effectively rescued defects than either wnt11 or wnt5 mRNA alone. However, the ectopic expression of wnt11 and wnt5 did not affect eaf1 and eaf2/u19 expression. These data indicate that eaf1 and eaf2/u19 act upstream of noncanonical Wnt signaling to mediate convergence and extension movements.; Studies have attributed several functions to the Eaf family, including tumor suppression and eye development. Given the potential association between cancer and development, we set forth to explore Eaf1 and Eaf2/U19 activity in vertebrate embryogenesis, using zebrafish. In situ hybridization revealed similar eaf1 and eaf2/u19 expression patterns. Morpholino-mediated knockdown of either eaf1 or eaf2/u19 expression produced similar morphological changes that could be reversed by ectopic expression of target or reciprocal-target mRNA. However, combination of Eaf1 and Eaf2/U19 (Eafs)-morpholinos increased the severity of defects, suggesting that Eaf1 and Eaf2/U19 only share some functional redundancy. The Eafs knockdown phenotype resembled that of embryos with defects in convergence and extension movements. Indeed, knockdown caused expression pattern changes for convergence and extension movement markers, whereas cell tracing experiments using kaeda mRNA showed a correlation between Eafs knockdown and cell migration defects. Cardiac and pancreatic differentiation markers revealed that Eafs knockdown also disrupted midline convergence of heart and pancreatic organ precursors. Noncanonical Wnt signaling plays a key role in both convergence and extension movements and midline convergence of organ precursors. We found that Eaf1 and Eaf2/U19 maintained expression levels of wnt11 and wnt5. Moreover, wnt11 or wnt5 mRNA partially rescued the convergence and extension movement defects occurring in eafs morphants. Wnt11 and Wnt5 converge on rhoA, so not surprisingly, rhoA mRNA more effectively rescued defects than either wnt11 or wnt5 mRNA alone. However, the ectopic expression of wnt11 and wnt5 did not affect eaf1 and eaf2/u19 expression. These data indicate that eaf1 and eaf2/u19 act upstream of noncanonical Wnt signaling to mediate convergence and extension movements.
SubtypeArticle
KeywordCell Movements Embryonic-development Axis Formation Factor-2 Eaf2 Gastrulation Ell Protein Roles Gene Mll
Department[Liu, Jing-Xia; Hu, Bo; Xiao, Wuhan] Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China; [Wang, Yang; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology
DOI10.1074/jbc.M109.009654
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China (NSFC) [30700440, 20890113]; Chinese Academy of Sciences [KSCX2-YW-N-020]; [2004CB117404]; [2007CB815705]; [2006CB504100] ; National Natural Science Foundation of China (NSFC) [30700440, 20890113]; Chinese Academy of Sciences [KSCX2-YW-N-020]; [2004CB117404]; [2007CB815705]; [2006CB504100] ; National Natural Science Foundation of China (NSFC) [30700440, 20890113]; Chinese Academy of Sciences [KSCX2-YW-N-020]; [2004CB117404]; [2007CB815705]; [2006CB504100] ; National Natural Science Foundation of China (NSFC) [30700440, 20890113]; Chinese Academy of Sciences [KSCX2-YW-N-020]; [2004CB117404]; [2007CB815705]; [2006CB504100]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000266730900063
WOS KeywordCELL MOVEMENTS ; EMBRYONIC-DEVELOPMENT ; AXIS FORMATION ; FACTOR-2 EAF2 ; GASTRULATION ; ELL ; PROTEIN ; ROLES ; GENE ; MLL
Funding OrganizationNational Natural Science Foundation of China (NSFC) [30700440, 20890113]; Chinese Academy of Sciences [KSCX2-YW-N-020]; [2004CB117404]; [2007CB815705]; [2006CB504100] ; National Natural Science Foundation of China (NSFC) [30700440, 20890113]; Chinese Academy of Sciences [KSCX2-YW-N-020]; [2004CB117404]; [2007CB815705]; [2006CB504100] ; National Natural Science Foundation of China (NSFC) [30700440, 20890113]; Chinese Academy of Sciences [KSCX2-YW-N-020]; [2004CB117404]; [2007CB815705]; [2006CB504100] ; National Natural Science Foundation of China (NSFC) [30700440, 20890113]; Chinese Academy of Sciences [KSCX2-YW-N-020]; [2004CB117404]; [2007CB815705]; [2006CB504100]
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7702
Collection期刊论文
Corresponding AuthorXiao, WH, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jing-Xia,Hu, Bo,Wang, Yang,et al. Zebrafish eaf1 and eaf2/u19 Mediate Effective Convergence and Extension Movements through the Maintenance of wnt11 and wnt5 Expression[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(24):16679-16692.
APA Liu, Jing-Xia,Hu, Bo,Wang, Yang,Gui, Jian-Fang,Xiao, Wuhan,&Xiao, WH, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.(2009).Zebrafish eaf1 and eaf2/u19 Mediate Effective Convergence and Extension Movements through the Maintenance of wnt11 and wnt5 Expression.JOURNAL OF BIOLOGICAL CHEMISTRY,284(24),16679-16692.
MLA Liu, Jing-Xia,et al."Zebrafish eaf1 and eaf2/u19 Mediate Effective Convergence and Extension Movements through the Maintenance of wnt11 and wnt5 Expression".JOURNAL OF BIOLOGICAL CHEMISTRY 284.24(2009):16679-16692.
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