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Medaka vasa is required for migration but not survival of primordial germ cells
Li, Mingyou; Hong, Ni; Xu, Hongyan; Yi, Meisheng; Li, Changming; Gui, Jianfang; Hong, Yunhan; Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore
2009
Source PublicationMECHANISMS OF DEVELOPMENT
Volume126Issue:5-6Pages:366-381
AbstractVasa is essential for germline development. However, the precise processes in which vasa involves vary considerably in diverse animal phyla. Here we show that vasa is required for primordial germ cell (PGC) migration in the medakafish. vasa knockdown by two morpholinos led to the PGC migration defect that was rescued by coinjection of Vasa RNA. Interestingly, Vasa knockdown did not alter the PGC number, identity, proliferation and motility even at ectopic locations. We established a cell culture system for tracing PGCs at the single cell level in vitro. In this culture system, control and morpholino-injected gastrulae produced the same PGC number and the same time course of PGC survival. importantly, vasa-depleted PGCs in culture had similar motility and locomotion to normal PGCs. Expression patterns of wt1a, sdf1b and cxcT4b in migratory tissues remained unchanged by Vasa knockdown. By chimera formation we show that PGCs from vasa-depleted blastulae failed to migrate properly in the normal environment, whereas control PGCs migrated normally in vasa-disrupted embryos. Furthermore, ectopic PGCs in vasa-depleted embryos also retained all the PGC properties examined. Taken together, medaka vasa is cell-autonomously required for PGC migration, but dispensable to PGC proliferation, motility, identity and survival. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
KeywordCell Migration Germ Cell Culture Morpholino Motility Oryzias Latipes Primordial Germ Cell Vasa
Department[Li, Mingyou; Hong, Ni; Xu, Hongyan; Yi, Meisheng; Hong, Yunhan] Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore; [Li, Mingyou; Gui, Jianfang; Hong, Yunhan] Wuhan Univ, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol,Ctr De, Wuhan 430072, Peoples R China; [Li, Changming] Nanyang Technol Univ, Sch Chem & Biomed Engn, Div Bioengn, Singapore 637457, Singapore
Subject AreaDevelopmental Biology
Indexed BySCI
Language英语
WOS IDWOS:000266975100008
Citation statistics
Cited Times:81[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7700
Collection期刊论文
Corresponding AuthorHong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore
Recommended Citation
GB/T 7714
Li, Mingyou,Hong, Ni,Xu, Hongyan,et al. Medaka vasa is required for migration but not survival of primordial germ cells[J]. MECHANISMS OF DEVELOPMENT,2009,126(5-6):366-381.
APA Li, Mingyou.,Hong, Ni.,Xu, Hongyan.,Yi, Meisheng.,Li, Changming.,...&Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore.(2009).Medaka vasa is required for migration but not survival of primordial germ cells.MECHANISMS OF DEVELOPMENT,126(5-6),366-381.
MLA Li, Mingyou,et al."Medaka vasa is required for migration but not survival of primordial germ cells".MECHANISMS OF DEVELOPMENT 126.5-6(2009):366-381.
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