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IgM, IgD and IgY and their expression pattern in the Chinese soft-shelled turtle Pelodiscus sinensis
Xu, Zhen1,2; Wang, Gai L.1; Nie, P.1; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2009-06-01
Source PublicationMOLECULAR IMMUNOLOGY
ISSN0161-5890
Volume46Issue:10Pages:2124-2132
AbstractThree Ig isotypes, IgM, IgD, and IgA, were previously known in reptiles. Here, in this report we describe IgM, IgD and a novel immunoglobulin heavy-chain isotype upsilon (IgY) in Chinese soft-shelled turtle (Pelodiscus sinensis). The IgM and IgY constant domains are characteristically similar to their counterparts described in other vertebrates. The expression of IgM and IgD were detected at mRNA level early during embryonic development, and their expression increased during further development. However, the IgY expression was not detected in larval turtles until 90 days after hatching-out. The increase in the transcription of these three Ig molecules was analyzed by using real-time PCR in spleen, kidney and blood following the injection of inactivated Aeromonas hydrophila. The primary increase in the expression of these three Igs was observed I week after the first injection, although not statistically significant, and the second injection 2 weeks after the first injection provoked a significant increase in the expression of these Igs, revealing a pattern of primary and secondary antibody response in the turtle. The present study represents the first report on reptile IgY and the pattern of IgM, IgD and IgY transcription in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.; Three Ig isotypes, IgM, IgD, and IgA, were previously known in reptiles. Here, in this report we describe IgM, IgD and a novel immunoglobulin heavy-chain isotype upsilon (IgY) in Chinese soft-shelled turtle (Pelodiscus sinensis). The IgM and IgY constant domains are characteristically similar to their counterparts described in other vertebrates. The expression of IgM and IgD were detected at mRNA level early during embryonic development, and their expression increased during further development. However, the IgY expression was not detected in larval turtles until 90 days after hatching-out. The increase in the transcription of these three Ig molecules was analyzed by using real-time PCR in spleen, kidney and blood following the injection of inactivated Aeromonas hydrophila. The primary increase in the expression of these three Igs was observed I week after the first injection, although not statistically significant, and the second injection 2 weeks after the first injection provoked a significant increase in the expression of these Igs, revealing a pattern of primary and secondary antibody response in the turtle. The present study represents the first report on reptile IgY and the pattern of IgM, IgD and IgY transcription in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordIgm Igd Igy Ontogeny Antibody Immune Response Chinese Soft-shelled Turtle
Department[Xu, Zhen; Wang, Gai L.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Xu, Zhen] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Immunology
DOI10.1016/j.molimm.2009.03.028
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [U0631010, 30571412] ; National Natural Science Foundation of China [U0631010, 30571412] ; National Natural Science Foundation of China [U0631010, 30571412] ; National Natural Science Foundation of China [U0631010, 30571412]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Immunology
WOS SubjectBiochemistry & Molecular Biology ; Immunology
WOS IDWOS:000266997500024
WOS KeywordIMMUNOGLOBULIN HEAVY-CHAIN ; CONSTANT-REGION GENE ; AMINO-ACID SEQUENCE ; NUCLEOTIDE-SEQUENCE ; MESSENGER-RNA ; ORGANIZATION ; ISOTYPE ; CLONING ; LOCUS ; DUCK
Funding OrganizationNational Natural Science Foundation of China [U0631010, 30571412] ; National Natural Science Foundation of China [U0631010, 30571412] ; National Natural Science Foundation of China [U0631010, 30571412] ; National Natural Science Foundation of China [U0631010, 30571412]
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7696
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Xu, Zhen,Wang, Gai L.,Nie, P.,et al. IgM, IgD and IgY and their expression pattern in the Chinese soft-shelled turtle Pelodiscus sinensis[J]. MOLECULAR IMMUNOLOGY,2009,46(10):2124-2132.
APA Xu, Zhen,Wang, Gai L.,Nie, P.,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.(2009).IgM, IgD and IgY and their expression pattern in the Chinese soft-shelled turtle Pelodiscus sinensis.MOLECULAR IMMUNOLOGY,46(10),2124-2132.
MLA Xu, Zhen,et al."IgM, IgD and IgY and their expression pattern in the Chinese soft-shelled turtle Pelodiscus sinensis".MOLECULAR IMMUNOLOGY 46.10(2009):2124-2132.
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