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学科主题: Biochemistry & Molecular Biology; Immunology
题名: IgM, IgD and IgY and their expression pattern in the Chinese soft-shelled turtle Pelodiscus sinensis
作者: Xu, Zhen1, 2; Wang, Gai L.1; Nie, P.1
通讯作者: Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
关键词: IgM ; IgD ; IgY ; Ontogeny ; Antibody ; Immune response ; Chinese soft-shelled turtle
刊名: MOLECULAR IMMUNOLOGY
发表日期: 2009-06-01
DOI: 10.1016/j.molimm.2009.03.028
卷: 46, 期:10, 页:2124-2132
收录类别: SCI
文章类型: Article
部门归属: [Xu, Zhen; Wang, Gai L.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Xu, Zhen] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [U0631010, 30571412]
类目[WOS]: Biochemistry & Molecular Biology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Immunology
摘要: Three Ig isotypes, IgM, IgD, and IgA, were previously known in reptiles. Here, in this report we describe IgM, IgD and a novel immunoglobulin heavy-chain isotype upsilon (IgY) in Chinese soft-shelled turtle (Pelodiscus sinensis). The IgM and IgY constant domains are characteristically similar to their counterparts described in other vertebrates. The expression of IgM and IgD were detected at mRNA level early during embryonic development, and their expression increased during further development. However, the IgY expression was not detected in larval turtles until 90 days after hatching-out. The increase in the transcription of these three Ig molecules was analyzed by using real-time PCR in spleen, kidney and blood following the injection of inactivated Aeromonas hydrophila. The primary increase in the expression of these three Igs was observed I week after the first injection, although not statistically significant, and the second injection 2 weeks after the first injection provoked a significant increase in the expression of these Igs, revealing a pattern of primary and secondary antibody response in the turtle. The present study represents the first report on reptile IgY and the pattern of IgM, IgD and IgY transcription in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要: Three Ig isotypes, IgM, IgD, and IgA, were previously known in reptiles. Here, in this report we describe IgM, IgD and a novel immunoglobulin heavy-chain isotype upsilon (IgY) in Chinese soft-shelled turtle (Pelodiscus sinensis). The IgM and IgY constant domains are characteristically similar to their counterparts described in other vertebrates. The expression of IgM and IgD were detected at mRNA level early during embryonic development, and their expression increased during further development. However, the IgY expression was not detected in larval turtles until 90 days after hatching-out. The increase in the transcription of these three Ig molecules was analyzed by using real-time PCR in spleen, kidney and blood following the injection of inactivated Aeromonas hydrophila. The primary increase in the expression of these three Igs was observed I week after the first injection, although not statistically significant, and the second injection 2 weeks after the first injection provoked a significant increase in the expression of these Igs, revealing a pattern of primary and secondary antibody response in the turtle. The present study represents the first report on reptile IgY and the pattern of IgM, IgD and IgY transcription in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.
关键词[WOS]: IMMUNOGLOBULIN HEAVY-CHAIN ; CONSTANT-REGION GENE ; AMINO-ACID SEQUENCE ; NUCLEOTIDE-SEQUENCE ; MESSENGER-RNA ; ORGANIZATION ; ISOTYPE ; CLONING ; LOCUS ; DUCK
语种: 英语
WOS记录号: WOS:000266997500024
ISSN号: 0161-5890
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7696
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China

Recommended Citation:
Xu, Zhen; Wang, Gai L.; Nie, P..IgM, IgD and IgY and their expression pattern in the Chinese soft-shelled turtle Pelodiscus sinensis,MOLECULAR IMMUNOLOGY,2009,46(10):2124-2132
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