IHB OpenIR  > 期刊论文
Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins
Xiong, Qian1,2; Xie, Ping1; Li, Huiying1; Hao, Le1,2; Li, Guangyu1,2; Qiu, Tong1,2; Liu, Ying1,2; Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009-07-01
Source PublicationTOXICON
ISSN0041-0101
Volume54Issue:1Pages:1-7
AbstractPrevious studies have shown that gonads were the second target organ of microcystins (MCs), and that MCs exposure exerted obvious toxic effects on male reproductive system of mammals. However, relevant molecular evidences are still lacking. Fas-signaling pathway plays a key role in toxicant-induced germ cell apoptosis. This study was to evaluate the responses of Fas/FasL system related genes and proteins in testes of rats injected intravenously with MCs. Enhanced apoptosis of germ cells in the testes of MCs-treated rats was detected by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) associated with up-regulation of the Fas/FasL system. Both Fas and FasL protein expression were induced evidently from I h post-injection, and this high expression level maintained throughout the experiment. In addition, the activation of caspase-8 and caspase-3 protein was also observed, which were indicators of apoptosis. These results suggested the likely involvement of Fas/FasL system in the MCs-induced germ cell apoptosis. It is also suggested that MCs can cause damage to Sertoli cells directly. (C) 2009 Elsevier Ltd. All rights reserved.; Previous studies have shown that gonads were the second target organ of microcystins (MCs), and that MCs exposure exerted obvious toxic effects on male reproductive system of mammals. However, relevant molecular evidences are still lacking. Fas-signaling pathway plays a key role in toxicant-induced germ cell apoptosis. This study was to evaluate the responses of Fas/FasL system related genes and proteins in testes of rats injected intravenously with MCs. Enhanced apoptosis of germ cells in the testes of MCs-treated rats was detected by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) associated with up-regulation of the Fas/FasL system. Both Fas and FasL protein expression were induced evidently from I h post-injection, and this high expression level maintained throughout the experiment. In addition, the activation of caspase-8 and caspase-3 protein was also observed, which were indicators of apoptosis. These results suggested the likely involvement of Fas/FasL system in the MCs-induced germ cell apoptosis. It is also suggested that MCs can cause damage to Sertoli cells directly. (C) 2009 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMicrocystin Fas/fasl Caspase Testis Tunel Staining Real-time Pcr Western Blot
Department[Xiong, Qian; Xie, Ping; Li, Huiying; Hao, Le; Li, Guangyu; Qiu, Tong; Liu, Ying] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Xiong, Qian; Hao, Le; Li, Guangyu; Qiu, Tong; Liu, Ying] Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
Subject AreaPharmacology & Pharmacy ; Toxicology
DOI10.1016/j.toxicon.2009.01.035
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101]
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000267011600001
WOS KeywordFAS LIGAND ; MONO-(2-ETHYLHEXYL) PHTHALATE ; TISSUE DISTRIBUTIONS ; REPRODUCTIVE-SYSTEM ; SEASONAL DYNAMICS ; SUBTROPICAL CHINA ; EUTROPHIC LAKE ; LARGE SHALLOW ; IN-VITRO ; LR
Funding OrganizationNational Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101] ; National Basic Research Program of China [2008CB418101]
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7692
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Qian,Xie, Ping,Li, Huiying,et al. Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins[J]. TOXICON,2009,54(1):1-7.
APA Xiong, Qian.,Xie, Ping.,Li, Huiying.,Hao, Le.,Li, Guangyu.,...&Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins.TOXICON,54(1),1-7.
MLA Xiong, Qian,et al."Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins".TOXICON 54.1(2009):1-7.
Files in This Item:
File Name/Size DocType Version Access License
Involvement of Fas-F(425KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xiong, Qian]'s Articles
[Xie, Ping]'s Articles
[Li, Huiying]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xiong, Qian]'s Articles
[Xie, Ping]'s Articles
[Li, Huiying]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xiong, Qian]'s Articles
[Xie, Ping]'s Articles
[Li, Huiying]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Involvement of Fas-FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.