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The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss
Gao, Q.1,2; Nie, P.2; Thompson, K. D.3; Adams, A.; Wang, T.1; Secombes, C. J.1; Zou, J.1; Zou, J, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
2009-08-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume33Issue:8Pages:920-931
AbstractInterferons (IFNs), consisting of three major subfamilies, type I, type II (gamma) and type III (lambda) IFN, activate vertebrate antiviral defences once bound to their receptors. The three IFN subfamilies bind to different receptors, IFNAR1 and IFNAR2 for type I IFNs, IFN gamma R1 and IFN gamma R2 for type II IFN, and IL-28R1 and IL-10R2 for type III IFNs. In fish, although many types I and II IFN genes have been cloned, little is known about their receptors. In this report, two putative IFN-gamma receptor chains were identified and sequenced in rainbow trout (Oncorhynchus mykiss), and found to have many common characteristics with mammalian type II IFN receptor family members. The presented gene synteny analysis, phylogenetic tree analysis and ligand binding analysis all suggest that these molecules are the authentic IFN gamma Rs in fish. They are widely expressed in tissues, with IFN gamma R1 typically more highly expressed than IFN gamma R2. Using the trout RTG-2 cell line it was possible to show that the individual chains could be differentially modulated, with rIFN-gamma and rIL-1 beta down regulating IFN gamma R1 expression but up regulating IFN gamma R2 expression. Overexpression of the two receptor chains in RTG-2 cells revealed that the level of IFN gamma R2 transcript was crucial for responsiveness to rIFN-gamma, in terms of inducing gamma IP expression. Transfection experiments showed that the two putative receptors specifically bound to rIFN-gamma. These findings are discussed in the context of how the IFN gamma R may bind IFN-gamma in fish and the importance of the individual receptor chains to signal transduction. (c) 2009 Elsevier Ltd. All rights reserved.; Interferons (IFNs), consisting of three major subfamilies, type I, type II (gamma) and type III (lambda) IFN, activate vertebrate antiviral defences once bound to their receptors. The three IFN subfamilies bind to different receptors, IFNAR1 and IFNAR2 for type I IFNs, IFN gamma R1 and IFN gamma R2 for type II IFN, and IL-28R1 and IL-10R2 for type III IFNs. In fish, although many types I and II IFN genes have been cloned, little is known about their receptors. In this report, two putative IFN-gamma receptor chains were identified and sequenced in rainbow trout (Oncorhynchus mykiss), and found to have many common characteristics with mammalian type II IFN receptor family members. The presented gene synteny analysis, phylogenetic tree analysis and ligand binding analysis all suggest that these molecules are the authentic IFN gamma Rs in fish. They are widely expressed in tissues, with IFN gamma R1 typically more highly expressed than IFN gamma R2. Using the trout RTG-2 cell line it was possible to show that the individual chains could be differentially modulated, with rIFN-gamma and rIL-1 beta down regulating IFN gamma R1 expression but up regulating IFN gamma R2 expression. Overexpression of the two receptor chains in RTG-2 cells revealed that the level of IFN gamma R2 transcript was crucial for responsiveness to rIFN-gamma, in terms of inducing gamma IP expression. Transfection experiments showed that the two putative receptors specifically bound to rIFN-gamma. These findings are discussed in the context of how the IFN gamma R may bind IFN-gamma in fish and the importance of the individual receptor chains to signal transduction. (c) 2009 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordIfn-gamma Receptor Rainbow Trout Characterization Expression Analysis
Department[Gao, Q.; Wang, T.; Secombes, C. J.; Zou, J.] Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland; [Gao, Q.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Thompson, K. D.] Univ Stirling, Inst Aquaculture, Stirling FP9 4LA, Scotland
Subject AreaImmunology ; Zoology
DOI10.1016/j.dci.2009.03.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationRoyal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1] ; Royal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1] ; Royal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1] ; Royal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000267004700005
WOS KeywordART. NO. 29 ; BETA-CHAIN ; CELL-LINE ; IDENTIFICATION ; GENE ; TUBERCULOSIS ; VERTEBRATES ; MODULATION ; ZEBRAFISH ; EVOLUTION
Funding OrganizationRoyal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1] ; Royal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1] ; Royal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1] ; Royal Society International Incoming Fellowship ; K C Wang Education Foundation ; Chinese Academy of Sciences ; BBSRC [BB/C506021/1]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7686
Collection期刊论文
Corresponding AuthorZou, J, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
Affiliation1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Univ Stirling, Inst Aquaculture, Stirling FP9 4LA, Scotland
Recommended Citation
GB/T 7714
Gao, Q.,Nie, P.,Thompson, K. D.,et al. The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(8):920-931.
APA Gao, Q..,Nie, P..,Thompson, K. D..,Adams, A..,Wang, T..,...&Zou, J, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland.(2009).The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(8),920-931.
MLA Gao, Q.,et al."The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.8(2009):920-931.
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