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学科主题: Ecology; Marine & Freshwater Biology
题名: Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella
作者: Wei, Lili1, 2, 3; Sun, Baojian1, 2; Chang, MingXian1, 2; Liu, Yi1, 2; Nie, Pin1, 2
通讯作者: Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
关键词: Grass carp ; Immune-relate genes ; Microcystin-LR ; Immunotoxicity ; Quantitative real-time PCR
刊名: ENVIRONMENTAL BIOLOGY OF FISHES
发表日期: 2009-07-01
DOI: 10.1007/s10641-009-9488-1
卷: 85, 期:3, 页:231-238
收录类别: SCI
文章类型: Article
部门归属: [Wei, Lili; Sun, Baojian; Chang, MingXian; Liu, Yi; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Wei, Lili; Sun, Baojian; Chang, MingXian; Liu, Yi; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China; [Wei, Lili] Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Jianxi Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [20577065]
类目[WOS]: Ecology ; Marine & Freshwater Biology
研究领域[WOS]: Environmental Sciences & Ecology ; Marine & Freshwater Biology
摘要: Recent studies in mammals have revealed that the cyanobacterial toxin MC-LR suppresses immune functions. Nevertheless, immunotoxic effects of microcystins have been little studied in fish. In this paper, we present the profiles of the immune modulation of MC-LR in grass carp, and quantitative real-time PCR methodology was developed for the measurement of relative transcription changes of six immune-related genes in the spleen and head kidney of the grass carp Ctenopharyngodon idella, which were intraperitoneally injected with 50 mu g MC-LR center dot kg(-1) body weight in a three-week period. This study was focused exclusively on gene transcription level changes at different time points after MC-LR exposure, so, only one dose was given. The investigated genes were interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), type I interferon (Type I IFN), peptidoglycan recognition protein-L (PGRP-L), immunoglobulin M (IgM) and major histocompatibility complex class I (MHC-I) genes. The results demonstrated that the transcription levels of the TNF-alpha, type I IFN, and PGRP-L genes in the spleen and head kidney were significantly low at all time points, and those of IL-1 beta were significantly low in the head kidney at different time points. In addition, IgM and MHC-I transcription levels were only significantly low in the spleen and head kidney at 21 d postinjection. The changes in the transcription levels of immune-related genes induced by MC-LR confirmed its effect on inhibiting immune function at the transcription level.
英文摘要: Recent studies in mammals have revealed that the cyanobacterial toxin MC-LR suppresses immune functions. Nevertheless, immunotoxic effects of microcystins have been little studied in fish. In this paper, we present the profiles of the immune modulation of MC-LR in grass carp, and quantitative real-time PCR methodology was developed for the measurement of relative transcription changes of six immune-related genes in the spleen and head kidney of the grass carp Ctenopharyngodon idella, which were intraperitoneally injected with 50 mu g MC-LR center dot kg(-1) body weight in a three-week period. This study was focused exclusively on gene transcription level changes at different time points after MC-LR exposure, so, only one dose was given. The investigated genes were interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), type I interferon (Type I IFN), peptidoglycan recognition protein-L (PGRP-L), immunoglobulin M (IgM) and major histocompatibility complex class I (MHC-I) genes. The results demonstrated that the transcription levels of the TNF-alpha, type I IFN, and PGRP-L genes in the spleen and head kidney were significantly low at all time points, and those of IL-1 beta were significantly low in the head kidney at different time points. In addition, IgM and MHC-I transcription levels were only significantly low in the spleen and head kidney at 21 d postinjection. The changes in the transcription levels of immune-related genes induced by MC-LR confirmed its effect on inhibiting immune function at the transcription level.
关键词[WOS]: PEPTIDOGLYCAN RECOGNITION PROTEINS ; NECROSIS-FACTOR-ALPHA ; CULTURED RAT HEPATOCYTES ; CYCLIC PEPTIDE TOXIN ; BLUE-GREEN-ALGAE ; TELEOST FISH ; INNATE IMMUNITY ; TUMOR PROMOTER ; CYCLOSPORINE-A ; MESSENGER-RNA
语种: 英语
WOS记录号: WOS:000267148300008
ISSN号: 0378-1909
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7684
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China
3.Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Jianxi Province, Peoples R China

Recommended Citation:
Wei, Lili; Sun, Baojian; Chang, MingXian; Liu, Yi; Nie, Pin.Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella,ENVIRONMENTAL BIOLOGY OF FISHES,2009,85(3):231-238
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