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Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella
Wei, Lili1,2,3; Sun, Baojian1,2; Chang, MingXian1,2; Liu, Yi1,2; Nie, Pin1,2; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2009-07-01
Source PublicationENVIRONMENTAL BIOLOGY OF FISHES
ISSN0378-1909
Volume85Issue:3Pages:231-238
AbstractRecent studies in mammals have revealed that the cyanobacterial toxin MC-LR suppresses immune functions. Nevertheless, immunotoxic effects of microcystins have been little studied in fish. In this paper, we present the profiles of the immune modulation of MC-LR in grass carp, and quantitative real-time PCR methodology was developed for the measurement of relative transcription changes of six immune-related genes in the spleen and head kidney of the grass carp Ctenopharyngodon idella, which were intraperitoneally injected with 50 mu g MC-LR center dot kg(-1) body weight in a three-week period. This study was focused exclusively on gene transcription level changes at different time points after MC-LR exposure, so, only one dose was given. The investigated genes were interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), type I interferon (Type I IFN), peptidoglycan recognition protein-L (PGRP-L), immunoglobulin M (IgM) and major histocompatibility complex class I (MHC-I) genes. The results demonstrated that the transcription levels of the TNF-alpha, type I IFN, and PGRP-L genes in the spleen and head kidney were significantly low at all time points, and those of IL-1 beta were significantly low in the head kidney at different time points. In addition, IgM and MHC-I transcription levels were only significantly low in the spleen and head kidney at 21 d postinjection. The changes in the transcription levels of immune-related genes induced by MC-LR confirmed its effect on inhibiting immune function at the transcription level.; Recent studies in mammals have revealed that the cyanobacterial toxin MC-LR suppresses immune functions. Nevertheless, immunotoxic effects of microcystins have been little studied in fish. In this paper, we present the profiles of the immune modulation of MC-LR in grass carp, and quantitative real-time PCR methodology was developed for the measurement of relative transcription changes of six immune-related genes in the spleen and head kidney of the grass carp Ctenopharyngodon idella, which were intraperitoneally injected with 50 mu g MC-LR center dot kg(-1) body weight in a three-week period. This study was focused exclusively on gene transcription level changes at different time points after MC-LR exposure, so, only one dose was given. The investigated genes were interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), type I interferon (Type I IFN), peptidoglycan recognition protein-L (PGRP-L), immunoglobulin M (IgM) and major histocompatibility complex class I (MHC-I) genes. The results demonstrated that the transcription levels of the TNF-alpha, type I IFN, and PGRP-L genes in the spleen and head kidney were significantly low at all time points, and those of IL-1 beta were significantly low in the head kidney at different time points. In addition, IgM and MHC-I transcription levels were only significantly low in the spleen and head kidney at 21 d postinjection. The changes in the transcription levels of immune-related genes induced by MC-LR confirmed its effect on inhibiting immune function at the transcription level.
SubtypeArticle
KeywordGrass Carp Immune-relate Genes Microcystin-lr Immunotoxicity Quantitative Real-time Pcr
Department[Wei, Lili; Sun, Baojian; Chang, MingXian; Liu, Yi; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Wei, Lili; Sun, Baojian; Chang, MingXian; Liu, Yi; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China; [Wei, Lili] Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Jianxi Province, Peoples R China
Subject AreaEcology ; Marine & Freshwater Biology
DOI10.1007/s10641-009-9488-1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
WOS SubjectEcology ; Marine & Freshwater Biology
WOS IDWOS:000267148300008
WOS KeywordPEPTIDOGLYCAN RECOGNITION PROTEINS ; NECROSIS-FACTOR-ALPHA ; CULTURED RAT HEPATOCYTES ; CYCLIC PEPTIDE TOXIN ; BLUE-GREEN-ALGAE ; TELEOST FISH ; INNATE IMMUNITY ; TUMOR PROMOTER ; CYCLOSPORINE-A ; MESSENGER-RNA
Funding OrganizationNational Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065] ; National Natural Science Foundation of China [20577065]
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7684
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China
3.Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Jianxi Province, Peoples R China
Recommended Citation
GB/T 7714
Wei, Lili,Sun, Baojian,Chang, MingXian,et al. Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella[J]. ENVIRONMENTAL BIOLOGY OF FISHES,2009,85(3):231-238.
APA Wei, Lili,Sun, Baojian,Chang, MingXian,Liu, Yi,Nie, Pin,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.(2009).Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella.ENVIRONMENTAL BIOLOGY OF FISHES,85(3),231-238.
MLA Wei, Lili,et al."Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella".ENVIRONMENTAL BIOLOGY OF FISHES 85.3(2009):231-238.
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