中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
学科主题: Marine & Freshwater Biology; Toxicology
题名: Hexabromocyclododecane-induced developmental toxicity and apoptosis in zebrafish embryos
作者: Deng, Jun1, 2; Yu, Liqin1, 2; Liu, Chunsheng1; Yu, Ke1; Shi, Xiongjie1; Yeung, Leo W. Y.3; Lam, Paul K. S.3; Wu, Rudolf S. S.3; Zhou, Bingsheng1
通讯作者: Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu S Rd 7, Wuhan 430072, Peoples R China
关键词: HBCD ; Developmental toxicity ; Apoptosis ; Zebrafish embryo ; Gene expression
刊名: AQUATIC TOXICOLOGY
发表日期: 2009-06-04
DOI: 10.1016/j.aquatox.2009.03.001
卷: 93, 期:1, 页:29-36
收录类别: SCI
文章类型: Article
部门归属: [Deng, Jun; Yu, Liqin; Liu, Chunsheng; Yu, Ke; Shi, Xiongjie; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Deng, Jun; Yu, Liqin] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Yeung, Leo W. Y.; Lam, Paul K. S.; Wu, Rudolf S. S.] City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Ministry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10]
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: Hexabromocyclododecane (HBCD) is widely used as a brominated flame retardant, and has been detected in the aquatic environment, wild animals, and humans. However, details of the environmental health risk of HBCD are not well known. In this study, zebrafish embryos were used to assess the developmental toxicity of the chemical. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to various concentrations of HBCD (0, 0.05, 0.1, 0.5, and 1.0 mg L-1) until 96 h. Exposure to 0.1, 0.5, and 1.0 mg L-1 HBCD significantly increased the malformation rate and reduced survival in the 0.5 and 1.0 mg L-1 HBCD exposure groups. Acridine orange (AO) staining showed that HBCD exposure resulted in cell apoptosis. Reactive oxygen species (ROS) was significantly induced at exposures of 0.1, 0.5, and 1.0 mg L-1 HBCD. To test the apoptotic pathway, several genes related to cell apoptosis, such as p53, Puma, Apaf-1, caspase-9, and caspase-3, were examined using real-time PCR. The expression patterns of these genes were up-regulated to some extent. Two anti-apoptotic genes, Mdm2 (antagonist of p53) and Bcl-2 (inhibitor of Bax), were down-regulated, and the activity of capspase-9 and caspase-3 was significantly increased. The overall results demonstrate that waterborne HBCD is able to produce oxidative stress and induce apoptosis through the involvement of caspases in zebrafish embryos. The results also indicate that zebrafish embryos can serve as a reliable model for the developmental toxicity of HBCD. (C) 2009 Elsevier B.V. All rights reserved.
英文摘要: Hexabromocyclododecane (HBCD) is widely used as a brominated flame retardant, and has been detected in the aquatic environment, wild animals, and humans. However, details of the environmental health risk of HBCD are not well known. In this study, zebrafish embryos were used to assess the developmental toxicity of the chemical. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to various concentrations of HBCD (0, 0.05, 0.1, 0.5, and 1.0 mg L(-1)) until 96 h. Exposure to 0.1, 0.5, and 1.0 mg L(-1) HBCD significantly increased the malformation rate and reduced survival in the 0.5 and 1.0 mg L(-1) HBCD exposure groups. Acridine orange (AO) staining showed that HBCD exposure resulted in cell apoptosis. Reactive oxygen species (ROS) was significantly induced at exposures of 0.1, 0.5, and 1.0 mg L(-1) HBCD. To test the apoptotic pathway, several genes related to cell apoptosis, such as p53, Puma, Apaf-1, caspase-9, and caspase-3, were examined using real-time PCR. The expression patterns of these genes were up-regulated to some extent. Two anti-apoptotic genes, Mdm2 (antagonist of p53) and Bcl-2 (inhibitor of Bax), were down-regulated, and the activity of capspase-9 and caspase-3 was significantly increased. The overall results demonstrate that waterborne HBCD is able to produce oxidative stress and induce apoptosis through the involvement of caspases in zebrafish embryos. The results also indicate that zebrafish embryos can serve as a reliable model for the developmental toxicity of HBCD. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: BROMINATED FLAME RETARDANTS ; TETRABROMOBISPHENOL-A TBBPA ; JUVENILE RAINBOW-TROUT ; GENE-EXPRESSION ; HEPATIC-ENZYMES ; CELL-DEATH ; HBCD ; TRENDS ; MODEL ; FISH
语种: 英语
WOS记录号: WOS:000267717800004
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7678
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Hexabromocyclododecane-induced developmental toxicity and apoptosis in zebrafish embryos.pdf(819KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Deng, Jun; Yu, Liqin; Liu, Chunsheng; Yu, Ke; Shi, Xiongjie; Yeung, Leo W. Y.; Lam, Paul K. S.; Wu, Rudolf S. S.; Zhou, Bingsheng.Hexabromocyclododecane-induced developmental toxicity and apoptosis in zebrafish embryos,AQUATIC TOXICOLOGY,2009,93(1):29-36
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Deng, Jun]'s Articles
[Yu, Liqin]'s Articles
[Liu, Chunsheng]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Deng, Jun]‘s Articles
[Yu, Liqin]‘s Articles
[Liu, Chunsheng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Hexabromocyclododecane-induced developmental toxicity and apoptosis in zebrafish embryos.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院水生生物研究所 - Feedback
Powered by CSpace