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Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila
Fu, Chengjie1; Xiong, Jie2,3; Miao, Wei1; Miao, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2009-05-01
Source PublicationBMC GENOMICS
ISSN1471-2164
Volume10Issue:0
AbstractBackground: Cytochrome P450 monooxygenases play key roles in the metabolism of a wide variety of substrates and they are closely associated with endocellular physiological processes or detoxification metabolism under environmental exposure. To date, however, none has been systematically characterized in the phylum Ciliophora. T. thermophila possess many advantages as a eukaryotic model organism and it exhibits rapid and sensitive responses to xenobiotics, making it an ideal model system to study the evolutionary and functional diversity of the P450 monooxygenase gene family. Results: A total of 44 putative functional cytochrome P450 genes were identified and could be classified into 13 families and 21 sub-families according to standard nomenclature. The characteristics of both the conserved intron-exon organization and scaffold localization of tandem repeats within each P450 family clade suggested that the enlargement of T. thermophila P450 families probably resulted from recent separate small duplication events. Gene expression patterns of all T. thermophila P450s during three important cell physiological stages (vegetative growth, starvation and conjugation) were analyzed based on EST and microarray data, and three main categories of expression patterns were postulated. Evolutionary analysis including codon usage preference, sit-especific selection and gene-expression evolution patterns were investigated and the results indicated remarkable divergences among the T. thermophila P450 genes. Conclusion: The characterization, expression and evolutionary analysis of T. thermophila P450 monooxygenase genes in the current study provides useful information for understanding the characteristics and diversities of the P450 genes in the Ciliophora, and provides the baseline for functional analyses of individual P450 isoforms in this model ciliate species.; Background: Cytochrome P450 monooxygenases play key roles in the metabolism of a wide variety of substrates and they are closely associated with endocellular physiological processes or detoxification metabolism under environmental exposure. To date, however, none has been systematically characterized in the phylum Ciliophora. T. thermophila possess many advantages as a eukaryotic model organism and it exhibits rapid and sensitive responses to xenobiotics, making it an ideal model system to study the evolutionary and functional diversity of the P450 monooxygenase gene family.
SubtypeArticle
KeywordSuppression Subtractive Hybridization Synonymous Codon Usage Sequence Tags Ests Caenorhabditis-elegans Paramecium-tetraurelia Maximum-likelihood Mutational Biases Nuclear Receptors Expressed Genes Amino-acid
Department[Fu, Chengjie; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Xiong, Jie] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Xiong, Jie] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
DOI10.1186/1471-2164-10-208
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMinistry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752] ; Ministry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752] ; Ministry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752] ; Ministry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000267734500001
WOS KeywordSUPPRESSION SUBTRACTIVE HYBRIDIZATION ; SYNONYMOUS CODON USAGE ; SEQUENCE TAGS ESTS ; CAENORHABDITIS-ELEGANS ; PARAMECIUM-TETRAURELIA ; MAXIMUM-LIKELIHOOD ; MUTATIONAL BIASES ; NUCLEAR RECEPTORS ; EXPRESSED GENES ; AMINO-ACID
Funding OrganizationMinistry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752] ; Ministry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752] ; Ministry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752] ; Ministry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7676
Collection期刊论文
Corresponding AuthorMiao, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Fu, Chengjie,Xiong, Jie,Miao, Wei,et al. Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila[J]. BMC GENOMICS,2009,10(0).
APA Fu, Chengjie,Xiong, Jie,Miao, Wei,&Miao, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.(2009).Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila.BMC GENOMICS,10(0).
MLA Fu, Chengjie,et al."Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila".BMC GENOMICS 10.0(2009).
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