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学科主题: Biotechnology & Applied Microbiology; Genetics & Heredity
题名: Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila
作者: Fu, Chengjie1; Xiong, Jie2, 3; Miao, Wei1
通讯作者: Miao, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
关键词: SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; SYNONYMOUS CODON USAGE ; SEQUENCE TAGS ESTS ; CAENORHABDITIS-ELEGANS ; PARAMECIUM-TETRAURELIA ; MAXIMUM-LIKELIHOOD ; MUTATIONAL BIASES ; NUCLEAR RECEPTORS ; EXPRESSED GENES ; AMINO-ACID
刊名: BMC GENOMICS
发表日期: 2009-05-01
DOI: 10.1186/1471-2164-10-208
卷: 10, 期:0
收录类别: SCI
文章类型: Article
部门归属: [Fu, Chengjie; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Xiong, Jie] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Xiong, Jie] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Ministry of Science and Technology of China [2006DFA31960]; National Natural Science Foundation of China [30870356]; Sunshine Foundation of Wuhan City [20055003059-22]; National Institutes of Health [GM021793, GM072752]
类目[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
摘要: Background: Cytochrome P450 monooxygenases play key roles in the metabolism of a wide variety of substrates and they are closely associated with endocellular physiological processes or detoxification metabolism under environmental exposure. To date, however, none has been systematically characterized in the phylum Ciliophora. T. thermophila possess many advantages as a eukaryotic model organism and it exhibits rapid and sensitive responses to xenobiotics, making it an ideal model system to study the evolutionary and functional diversity of the P450 monooxygenase gene family. Results: A total of 44 putative functional cytochrome P450 genes were identified and could be classified into 13 families and 21 sub-families according to standard nomenclature. The characteristics of both the conserved intron-exon organization and scaffold localization of tandem repeats within each P450 family clade suggested that the enlargement of T. thermophila P450 families probably resulted from recent separate small duplication events. Gene expression patterns of all T. thermophila P450s during three important cell physiological stages (vegetative growth, starvation and conjugation) were analyzed based on EST and microarray data, and three main categories of expression patterns were postulated. Evolutionary analysis including codon usage preference, sit-especific selection and gene-expression evolution patterns were investigated and the results indicated remarkable divergences among the T. thermophila P450 genes. Conclusion: The characterization, expression and evolutionary analysis of T. thermophila P450 monooxygenase genes in the current study provides useful information for understanding the characteristics and diversities of the P450 genes in the Ciliophora, and provides the baseline for functional analyses of individual P450 isoforms in this model ciliate species.
英文摘要: Background: Cytochrome P450 monooxygenases play key roles in the metabolism of a wide variety of substrates and they are closely associated with endocellular physiological processes or detoxification metabolism under environmental exposure. To date, however, none has been systematically characterized in the phylum Ciliophora. T. thermophila possess many advantages as a eukaryotic model organism and it exhibits rapid and sensitive responses to xenobiotics, making it an ideal model system to study the evolutionary and functional diversity of the P450 monooxygenase gene family.
关键词[WOS]: SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; SYNONYMOUS CODON USAGE ; SEQUENCE TAGS ESTS ; CAENORHABDITIS-ELEGANS ; PARAMECIUM-TETRAURELIA ; MAXIMUM-LIKELIHOOD ; MUTATIONAL BIASES ; NUCLEAR RECEPTORS ; EXPRESSED GENES ; AMINO-ACID
语种: 英语
WOS记录号: WOS:000267734500001
ISSN号: 1471-2164
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7676
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Fu, Chengjie; Xiong, Jie; Miao, Wei.Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila,BMC GENOMICS,2009,10():
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