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Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus
Su, Jianguo1; Yang, Chunrong1; Xiong, Feng2; Wang, Yaping2; Zhu, Zuoyan2; Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Peoples R China
2009-07-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume27Issue:1Pages:33-39
AbstractToll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved.; Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordGobiocypris Rarus Grass Carp Reovirus Aeromonas Hydrophila Toll-like Receptor 4 Gene Cloning Characterization
Department[Su, Jianguo; Yang, Chunrong] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China; [Xiong, Feng; Wang, Yaping; Zhu, Zuoyan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2009.02.016
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000267478200005
WOS KeywordRESPIRATORY SYNCYTIAL VIRUS ; MESSENGER-RNA DEGRADATION ; INNATE IMMUNITY ; EXPRESSION ANALYSIS ; TLR4 POLYMORPHISMS ; ADAPTIVE IMMUNITY ; GENE FAMILY ; ENDOTOXIN ; SEQUENCE ; TIR
Funding OrganizationNational Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104]
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Cited Times:53[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7674
Collection期刊论文
Corresponding AuthorSu, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Peoples R China
Affiliation1.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Su, Jianguo,Yang, Chunrong,Xiong, Feng,et al. Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus[J]. FISH & SHELLFISH IMMUNOLOGY,2009,27(1):33-39.
APA Su, Jianguo,Yang, Chunrong,Xiong, Feng,Wang, Yaping,Zhu, Zuoyan,&Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Peoples R China.(2009).Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus.FISH & SHELLFISH IMMUNOLOGY,27(1),33-39.
MLA Su, Jianguo,et al."Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus".FISH & SHELLFISH IMMUNOLOGY 27.1(2009):33-39.
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