| Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus | |
| Su, Jianguo1; Yang, Chunrong1; Xiong, Feng2; Wang, Yaping2; Zhu, Zuoyan2; Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Peoples R China | |
| 2009-07-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 27Issue:1Pages:33-39 |
| Abstract | Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved.; Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Gobiocypris Rarus Grass Carp Reovirus Aeromonas Hydrophila Toll-like Receptor 4 Gene Cloning Characterization |
| Department | [Su, Jianguo; Yang, Chunrong] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China; [Xiong, Feng; Wang, Yaping; Zhu, Zuoyan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Subject Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2009.02.016 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000267478200005 |
| WOS Keyword | RESPIRATORY SYNCYTIAL VIRUS ; MESSENGER-RNA DEGRADATION ; INNATE IMMUNITY ; EXPRESSION ANALYSIS ; TLR4 POLYMORPHISMS ; ADAPTIVE IMMUNITY ; GENE FAMILY ; ENDOTOXIN ; SEQUENCE ; TIR |
| Funding Organization | National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] ; National Natural Science Foundation of China [30871917]; SRF [14110104] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7674 |
| Collection | 期刊论文 |
| Corresponding Author | Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Peoples R China |
| Affiliation | 1.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Su, Jianguo,Yang, Chunrong,Xiong, Feng,et al. Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus[J]. FISH & SHELLFISH IMMUNOLOGY,2009,27(1):33-39. |
| APA | Su, Jianguo,Yang, Chunrong,Xiong, Feng,Wang, Yaping,Zhu, Zuoyan,&Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Peoples R China.(2009).Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus.FISH & SHELLFISH IMMUNOLOGY,27(1),33-39. |
| MLA | Su, Jianguo,et al."Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus".FISH & SHELLFISH IMMUNOLOGY 27.1(2009):33-39. |
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