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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus
作者: Su, Jianguo1; Yang, Chunrong1; Xiong, Feng2; Wang, Yaping2; Zhu, Zuoyan2
通讯作者: Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Peoples R China
关键词: Gobiocypris rarus ; Grass carp reovirus ; Aeromonas hydrophila ; Toll-like receptor 4 ; Gene cloning ; Characterization
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2009-07-01
DOI: 10.1016/j.fsi.2009.02.016
卷: 27, 期:1, 页:33-39
收录类别: SCI
文章类型: Article
部门归属: [Su, Jianguo; Yang, Chunrong] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China; [Xiong, Feng; Wang, Yaping; Zhu, Zuoyan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30871917]; SRF [14110104]
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要: Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved.
关键词[WOS]: RESPIRATORY SYNCYTIAL VIRUS ; MESSENGER-RNA DEGRADATION ; INNATE IMMUNITY ; EXPRESSION ANALYSIS ; TLR4 POLYMORPHISMS ; ADAPTIVE IMMUNITY ; GENE FAMILY ; ENDOTOXIN ; SEQUENCE ; TIR
语种: 英语
WOS记录号: WOS:000267478200005
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7674
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Su, Jianguo; Yang, Chunrong; Xiong, Feng; Wang, Yaping; Zhu, Zuoyan.Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus,FISH & SHELLFISH IMMUNOLOGY,2009,27(1):33-39
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