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学科主题: Biochemistry & Molecular Biology; Fisheries; Physiology
题名: Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages
作者: Liao, T.1, 2; Guo, Q. L.1; Jin, S. W.3; Cheng, W.2; Xu, Y.1
通讯作者: Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu Lake S Rd, Wuhan 430064, Peoples R China
关键词: Biomarker ; Estradiol ; Hermaphrodism ; Sex ratio ; Vitellogenin
刊名: FISH PHYSIOLOGY AND BIOCHEMISTRY
发表日期: 2009-08-01
DOI: 10.1007/s10695-008-9247-9
卷: 35, 期:3, 页:341-349
收录类别: SCI
文章类型: Article
部门归属: [Liao, T.; Guo, Q. L.; Xu, Y.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430064, Peoples R China; [Liao, T.; Cheng, W.] Hubei Acad Agr Sci, Inst Farm Prod Proc & Nucl Agr Technol, Hubei Innovat Ctr Agr Sci & Technol, Wuhan 430064, Peoples R China; [Jin, S. W.] Wuhan Inst Technol, Key Lab Green Chem Proc, Minist Educ, Wuhan 430073, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Hi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005]
类目[WOS]: Biochemistry & Molecular Biology ; Fisheries ; Physiology
研究领域[WOS]: Biochemistry & Molecular Biology ; Fisheries ; Physiology
摘要: Present in the excrement of humans and animals, 17 beta-estradiol (E-2) has been detected in the aquatic environment in a range from several nanograms to several hundred nanograms per liter. In this study, the sensitivities of rare minnows during different life stages to E-2 at environmentally relevant (5, 25, and 100 ng l(-1)) and high (1000 ng l(-1)) concentrations were compared using vitellogenin (VTG) and gonad development as biomarkers under semistatic conditions. After 21 days of exposure, VTG concentrations in whole-body homogenates were analyzed; the results indicated that the lowest observed effective concentration for VTG induction was 25 ng l(-1) E-2 in the adult stage, but 100 ng l(-1) E-2 in the larval and juvenile stages. After exposure in the early life stage, the larval and juvenile fish were transferred to clean water until gonad maturation. No significant difference in VTG induction was found between the exposure and control groups in the adults. However, a markedly increased proportion of females and appearance of hermaphrodism were observed in the juvenile-stage group exposed to 25 ng l(-1) E-2. These results showed that VTG induction in the adult stage is more sensitive than in larval and juvenile stages following exposure to E-2. The juvenile stage may be the critical period of gonad development. Sex ratio could be a sensitive biomarker indicating exposure to xenoestrogens in early-life-stage subchronic exposure tests. The results of this study provide useful information for selecting sensitive biomarkers properly in aquatic toxicology testing.
英文摘要: Present in the excrement of humans and animals, 17 beta-estradiol (E(2)) has been detected in the aquatic environment in a range from several nanograms to several hundred nanograms per liter. In this study, the sensitivities of rare minnows during different life stages to E(2) at environmentally relevant (5, 25, and 100 ng l(-1)) and high (1000 ng l(-1)) concentrations were compared using vitellogenin (VTG) and gonad development as biomarkers under semistatic conditions. After 21 days of exposure, VTG concentrations in whole-body homogenates were analyzed; the results indicated that the lowest observed effective concentration for VTG induction was 25 ng l(-1) E(2) in the adult stage, but 100 ng l(-1) E(2) in the larval and juvenile stages. After exposure in the early life stage, the larval and juvenile fish were transferred to clean water until gonad maturation. No significant difference in VTG induction was found between the exposure and control groups in the adults. However, a markedly increased proportion of females and appearance of hermaphrodism were observed in the juvenile-stage group exposed to 25 ng l(-1) E(2). These results showed that VTG induction in the adult stage is more sensitive than in larval and juvenile stages following exposure to E(2). The juvenile stage may be the critical period of gonad development. Sex ratio could be a sensitive biomarker indicating exposure to xenoestrogens in early-life-stage subchronic exposure tests. The results of this study provide useful information for selecting sensitive biomarkers properly in aquatic toxicology testing.
关键词[WOS]: ZEBRAFISH DANIO-RERIO ; ENVIRONMENTALLY RELEVANT CONCENTRATIONS ; SEWAGE-TREATMENT PLANTS ; IN-VIVO ; GOBIOCYPRIS-RARUS ; VITELLOGENIN INDUCTION ; ENDOCRINE DISRUPTORS ; TRANSGENIC ZEBRAFISH ; ESTROGENIC CHEMICALS ; PIMEPHALES-PROMELAS
语种: 英语
WOS记录号: WOS:000267681000004
ISSN号: 0920-1742
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7672
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430064, Peoples R China
2.Hubei Acad Agr Sci, Inst Farm Prod Proc & Nucl Agr Technol, Hubei Innovat Ctr Agr Sci & Technol, Wuhan 430064, Peoples R China
3.Wuhan Inst Technol, Key Lab Green Chem Proc, Minist Educ, Wuhan 430073, Peoples R China

Recommended Citation:
Liao, T.; Guo, Q. L.; Jin, S. W.; Cheng, W.; Xu, Y..Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages,FISH PHYSIOLOGY AND BIOCHEMISTRY,2009,35(3):341-349
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