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Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages
Liao, T.1,2; Guo, Q. L.1; Jin, S. W.3; Cheng, W.2; Xu, Y.1; Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu Lake S Rd, Wuhan 430064, Peoples R China
2009-08-01
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
Volume35Issue:3Pages:341-349
AbstractPresent in the excrement of humans and animals, 17 beta-estradiol (E-2) has been detected in the aquatic environment in a range from several nanograms to several hundred nanograms per liter. In this study, the sensitivities of rare minnows during different life stages to E-2 at environmentally relevant (5, 25, and 100 ng l(-1)) and high (1000 ng l(-1)) concentrations were compared using vitellogenin (VTG) and gonad development as biomarkers under semistatic conditions. After 21 days of exposure, VTG concentrations in whole-body homogenates were analyzed; the results indicated that the lowest observed effective concentration for VTG induction was 25 ng l(-1) E-2 in the adult stage, but 100 ng l(-1) E-2 in the larval and juvenile stages. After exposure in the early life stage, the larval and juvenile fish were transferred to clean water until gonad maturation. No significant difference in VTG induction was found between the exposure and control groups in the adults. However, a markedly increased proportion of females and appearance of hermaphrodism were observed in the juvenile-stage group exposed to 25 ng l(-1) E-2. These results showed that VTG induction in the adult stage is more sensitive than in larval and juvenile stages following exposure to E-2. The juvenile stage may be the critical period of gonad development. Sex ratio could be a sensitive biomarker indicating exposure to xenoestrogens in early-life-stage subchronic exposure tests. The results of this study provide useful information for selecting sensitive biomarkers properly in aquatic toxicology testing.; Present in the excrement of humans and animals, 17 beta-estradiol (E(2)) has been detected in the aquatic environment in a range from several nanograms to several hundred nanograms per liter. In this study, the sensitivities of rare minnows during different life stages to E(2) at environmentally relevant (5, 25, and 100 ng l(-1)) and high (1000 ng l(-1)) concentrations were compared using vitellogenin (VTG) and gonad development as biomarkers under semistatic conditions. After 21 days of exposure, VTG concentrations in whole-body homogenates were analyzed; the results indicated that the lowest observed effective concentration for VTG induction was 25 ng l(-1) E(2) in the adult stage, but 100 ng l(-1) E(2) in the larval and juvenile stages. After exposure in the early life stage, the larval and juvenile fish were transferred to clean water until gonad maturation. No significant difference in VTG induction was found between the exposure and control groups in the adults. However, a markedly increased proportion of females and appearance of hermaphrodism were observed in the juvenile-stage group exposed to 25 ng l(-1) E(2). These results showed that VTG induction in the adult stage is more sensitive than in larval and juvenile stages following exposure to E(2). The juvenile stage may be the critical period of gonad development. Sex ratio could be a sensitive biomarker indicating exposure to xenoestrogens in early-life-stage subchronic exposure tests. The results of this study provide useful information for selecting sensitive biomarkers properly in aquatic toxicology testing.
SubtypeArticle
KeywordBiomarker Estradiol Hermaphrodism Sex Ratio Vitellogenin
Department[Liao, T.; Guo, Q. L.; Xu, Y.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430064, Peoples R China; [Liao, T.; Cheng, W.] Hubei Acad Agr Sci, Inst Farm Prod Proc & Nucl Agr Technol, Hubei Innovat Ctr Agr Sci & Technol, Wuhan 430064, Peoples R China; [Jin, S. W.] Wuhan Inst Technol, Key Lab Green Chem Proc, Minist Educ, Wuhan 430073, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
DOI10.1007/s10695-008-9247-9
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationHi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS SubjectBiochemistry & Molecular Biology ; Fisheries ; Physiology
WOS IDWOS:000267681000004
WOS KeywordZEBRAFISH DANIO-RERIO ; ENVIRONMENTALLY RELEVANT CONCENTRATIONS ; SEWAGE-TREATMENT PLANTS ; IN-VIVO ; GOBIOCYPRIS-RARUS ; VITELLOGENIN INDUCTION ; ENDOCRINE DISRUPTORS ; TRANSGENIC ZEBRAFISH ; ESTROGENIC CHEMICALS ; PIMEPHALES-PROMELAS
Funding OrganizationHi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Basic Research Program of China [2003CB415005]
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7672
Collection期刊论文
Corresponding AuthorXu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu Lake S Rd, Wuhan 430064, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430064, Peoples R China
2.Hubei Acad Agr Sci, Inst Farm Prod Proc & Nucl Agr Technol, Hubei Innovat Ctr Agr Sci & Technol, Wuhan 430064, Peoples R China
3.Wuhan Inst Technol, Key Lab Green Chem Proc, Minist Educ, Wuhan 430073, Peoples R China
Recommended Citation
GB/T 7714
Liao, T.,Guo, Q. L.,Jin, S. W.,et al. Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2009,35(3):341-349.
APA Liao, T.,Guo, Q. L.,Jin, S. W.,Cheng, W.,Xu, Y.,&Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu Lake S Rd, Wuhan 430064, Peoples R China.(2009).Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages.FISH PHYSIOLOGY AND BIOCHEMISTRY,35(3),341-349.
MLA Liao, T.,et al."Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages".FISH PHYSIOLOGY AND BIOCHEMISTRY 35.3(2009):341-349.
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