IHB OpenIR  > 期刊论文
Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)
Chen, Guo-Yuan1,2; Qiu, Shan-Lian1,2; Zhou, Yi-Yong1; Zhou, YY, Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, 7 Donghu Nan Rd, Wuhan 430072, Hubei, Peoples R China
2009-04-01
Source PublicationRESEARCH IN MICROBIOLOGY
ISSN0923-2508
Volume160Issue:3Pages:173-178
AbstractClassical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH4+) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved.; Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH(4)(+)) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved.
SubtypeArticle
KeywordAmmonia-oxidizing Bacteria Ammonia Monooxygenase Gene Restriction Fragment Length Polymorphism Shallow Lakes
Department[Chen, Guo-Yuan; Qiu, Shan-Lian; Zhou, Yi-Yong] Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Chen, Guo-Yuan; Qiu, Shan-Lian] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Subject AreaMicrobiology
DOI10.1016/j.resmic.2009.01.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000267685500001
WOS Keyword16S RIBOSOMAL-RNA ; FRESH-WATER LAKE ; IN-SITU HYBRIDIZATION ; NITRIFYING BACTERIA ; MOLECULAR DIVERSITY ; AMOA ; POPULATIONS ; OXIDATION ; GENE ; NITRIFICATION
Funding OrganizationNational Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7668
Collection期刊论文
Corresponding AuthorZhou, YY, Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, 7 Donghu Nan Rd, Wuhan 430072, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Chen, Guo-Yuan,Qiu, Shan-Lian,Zhou, Yi-Yong,et al. Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)[J]. RESEARCH IN MICROBIOLOGY,2009,160(3):173-178.
APA Chen, Guo-Yuan,Qiu, Shan-Lian,Zhou, Yi-Yong,&Zhou, YY, Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, 7 Donghu Nan Rd, Wuhan 430072, Hubei, Peoples R China.(2009).Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu).RESEARCH IN MICROBIOLOGY,160(3),173-178.
MLA Chen, Guo-Yuan,et al."Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)".RESEARCH IN MICROBIOLOGY 160.3(2009):173-178.
Files in This Item: Download All
File Name/Size DocType Version Access License
Diversity and abunda(420KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Guo-Yuan]'s Articles
[Qiu, Shan-Lian]'s Articles
[Zhou, Yi-Yong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Guo-Yuan]'s Articles
[Qiu, Shan-Lian]'s Articles
[Zhou, Yi-Yong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Guo-Yuan]'s Articles
[Qiu, Shan-Lian]'s Articles
[Zhou, Yi-Yong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu).pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.