| Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu) | |
| Chen, Guo-Yuan1,2; Qiu, Shan-Lian1,2; Zhou, Yi-Yong1; Zhou, YY, Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, 7 Donghu Nan Rd, Wuhan 430072, Hubei, Peoples R China | |
| 2009-04-01 | |
| Source Publication | RESEARCH IN MICROBIOLOGY
 |
| ISSN | 0923-2508 |
| Volume | 160Issue:3Pages:173-178 |
| Abstract | Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH4+) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved.; Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH(4)(+)) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved. |
| Subtype | Article |
| Keyword | Ammonia-oxidizing Bacteria Ammonia Monooxygenase Gene Restriction Fragment Length Polymorphism Shallow Lakes |
| Department | [Chen, Guo-Yuan; Qiu, Shan-Lian; Zhou, Yi-Yong] Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Chen, Guo-Yuan; Qiu, Shan-Lian] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| Subject Area | Microbiology |
| DOI | 10.1016/j.resmic.2009.01.003 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Microbiology |
| WOS Subject | Microbiology |
| WOS ID | WOS:000267685500001 |
| WOS Keyword | 16S RIBOSOMAL-RNA ; FRESH-WATER LAKE ; IN-SITU HYBRIDIZATION ; NITRIFYING BACTERIA ; MOLECULAR DIVERSITY ; AMOA ; POPULATIONS ; OXIDATION ; GENE ; NITRIFICATION |
| Funding Organization | National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7668 |
| Collection | 期刊论文 |
| Corresponding Author | Zhou, YY, Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, 7 Donghu Nan Rd, Wuhan 430072, Hubei, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Guo-Yuan,Qiu, Shan-Lian,Zhou, Yi-Yong,et al. Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)[J]. RESEARCH IN MICROBIOLOGY,2009,160(3):173-178. |
| APA | Chen, Guo-Yuan,Qiu, Shan-Lian,Zhou, Yi-Yong,&Zhou, YY, Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, 7 Donghu Nan Rd, Wuhan 430072, Hubei, Peoples R China.(2009).Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu).RESEARCH IN MICROBIOLOGY,160(3),173-178. |
| MLA | Chen, Guo-Yuan,et al."Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)".RESEARCH IN MICROBIOLOGY 160.3(2009):173-178. |
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