| Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity | |
| Cao, Xiuyun3; Song, Chunlei3; Zhou, Yiyong3; Strojsova, Alena1,2; Znachor, Petr1,2; Zapomelova, Eliska1,2; Vrba, Jaroslav1,2; Vrba, J, Univ S Bohemia, Fac Sci, Sadkach 7, Ceske Budejovice 37005, Czech Republic | |
| 2009-08-01 | |
| Source Publication | LIMNOLOGY
 |
| ISSN | 1439-8621 |
| Volume | 10Issue:2Pages:95-104 |
| Abstract | Extracellular phosphatases are an important part of the phosphorus cycle in aquatic environments. Phosphatase activity (PA) in plankton was studied in seven subtropical shallow lakes of different exploitation management and trophic status in the urban area of Wuhan City. Bulk PA was rather high (range 1.1-11 mu mol l(-1) h(-1)), although concentrations of soluble reactive phosphorus (SRP) were also high (range 27 mu g P l(-1) to similar to 1.5 mg P l(-1)) in all lakes. Cell-associated extracellular PA in phytoplankton was detected using the fluorescence-labelled enzyme activity technique. Phytoplankton species partly contributed to the bulk PA. We found explicit differences in the presence of cell-associated phosphatase within the main phytoplankton groups; species belonging to Chlorophyta and Dinophyta were regularly phosphatase-positive, while Cyanophyta and Bacillariophyceae were phosphatase-negative in all but one case. Furthermore, there is a certain potential of extracellular phosphatases produced by heterotrophic nanoflagellates in most of the lakes. This new finding compromises the 'traditional' interpretation of bulk phosphatase data as being due to overall phytoplankton or bacterial P regeneration.; Extracellular phosphatases are an important part of the phosphorus cycle in aquatic environments. Phosphatase activity (PA) in plankton was studied in seven subtropical shallow lakes of different exploitation management and trophic status in the urban area of Wuhan City. Bulk PA was rather high (range 1.1-11 mu mol l(-1) h(-1)), although concentrations of soluble reactive phosphorus (SRP) were also high (range 27 mu g P l(-1) to similar to 1.5 mg P l(-1)) in all lakes. Cell-associated extracellular PA in phytoplankton was detected using the fluorescence-labelled enzyme activity technique. Phytoplankton species partly contributed to the bulk PA. We found explicit differences in the presence of cell-associated phosphatase within the main phytoplankton groups; species belonging to Chlorophyta and Dinophyta were regularly phosphatase-positive, while Cyanophyta and Bacillariophyceae were phosphatase-negative in all but one case. Furthermore, there is a certain potential of extracellular phosphatases produced by heterotrophic nanoflagellates in most of the lakes. This new finding compromises the 'traditional' interpretation of bulk phosphatase data as being due to overall phytoplankton or bacterial P regeneration. |
| Subtype | Article |
| Keyword | Elf97 Phosphate Phosphorus Regeneration Polymictic Lakes Algae Heterotrophic Flagellates Protozoa |
| Department | [Strojsova, Alena; Znachor, Petr; Zapomelova, Eliska; Vrba, Jaroslav] Univ S Bohemia, Fac Sci, Ceske Budejovice 37005, Czech Republic; [Strojsova, Alena; Znachor, Petr; Zapomelova, Eliska; Vrba, Jaroslav] Acad Sci Czech Republic, Inst Hydrobiol, Ctr Biol, Ceske Budejovice 37005, Czech Republic; [Cao, Xiuyun; Song, Chunlei; Zhou, Yiyong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Subject Area | Limnology |
| DOI | 10.1007/s10201-009-0265-9 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617] ; National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617] ; National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617] ; National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Marine & Freshwater Biology |
| WOS Subject | Limnology |
| WOS ID | WOS:000268017800003 |
| WOS Keyword | PHOSPHORUS-DEFICIENCY INDICATORS ; ENZYME-LABELED FLUORESCENCE ; FRESH-WATER PHYTOPLANKTON ; ALKALINE-PHOSPHATASE ; SPRING PHYTOPLANKTON ; KINETICS ; DONGHU ; ERKEN ; AVAILABILITY ; ENVIRONMENTS |
| Funding Organization | National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617] ; National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617] ; National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617] ; National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7656 |
| Collection | 期刊论文 |
| Corresponding Author | Vrba, J, Univ S Bohemia, Fac Sci, Sadkach 7, Ceske Budejovice 37005, Czech Republic |
| Affiliation | 1.Univ S Bohemia, Fac Sci, Ceske Budejovice 37005, Czech Republic 2.Acad Sci Czech Republic, Inst Hydrobiol, Ctr Biol, Ceske Budejovice 37005, Czech Republic 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Cao, Xiuyun,Song, Chunlei,Zhou, Yiyong,et al. Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity[J]. LIMNOLOGY,2009,10(2):95-104. |
| APA | Cao, Xiuyun.,Song, Chunlei.,Zhou, Yiyong.,Strojsova, Alena.,Znachor, Petr.,...&Vrba, J, Univ S Bohemia, Fac Sci, Sadkach 7, Ceske Budejovice 37005, Czech Republic.(2009).Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity.LIMNOLOGY,10(2),95-104. |
| MLA | Cao, Xiuyun,et al."Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity".LIMNOLOGY 10.2(2009):95-104. |
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