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Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish
Liu, Chunsheng1,2; Yu, Liqin1; Deng, Jun1; Lam, Paul K. S.3; Wu, Rudolf S. S.3; Zhou, Bingsheng1; Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009-06-28
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume93Issue:2-3Pages:131-137
AbstractFluorotelomer alcohols (FTOHs) have shown estrogenic activity in vitro and in vivo, but the mechanism of this activity is not known. In this study, 18-week-old zebrafish (Danio rerio) were exposed to 0, 0.03, 0.3 and 3.0 mg/l 1H, 1H, 2H, 2H-perfluorooctan-1-ol (6:2 ETCH) for 7 days, and the effects on plasma sex hormone levels were measured followed by use of real-time PCR to examine selected gene expression in hypothalamic-pituitary-gonadal (HPG) axis and liver. Exposure to 6:2 FTOH significantly increased plasma estradiol (E2) and testosterone (T) levels in both males and females. Furthermore, the ratio of T/E2 was reduced in females while increased in males. In females, the increase of E2 was accompanied by up-regulated hepatic estrogenic receptor alpha (ER alpha) and vitellogenin (VTG1 and VTG3) expression. In males, the elevation of the T level is consistent with the up-regulation of cytochrome P450 c17 alpha-hydroxylase, 17, 20-lase (CYP17) and the down-regulation of cytochrome P450 aromatase A (CYP19A). The present study demonstrated that waterborne exposure to 6:2 FTOH alter plasma sex hormone levels and the ratio of T/E2, as well as the transcriptional profiles of some genes in the HPG axis and liver. The results suggested that FTOHs may disturb fish reproduction through endocrine disrupted activity. (C) 2009 Elsevier B.V. All rights reserved.; Fluorotelomer alcohols (FTOHs) have shown estrogenic activity in vitro and in vivo, but the mechanism of this activity is not known. In this study, 18-week-old zebrafish (Danio rerio) were exposed to 0, 0.03, 0.3 and 3.0 mg/l 1H, 1H, 2H, 2H-perfluorooctan-1-ol (6:2 ETCH) for 7 days, and the effects on plasma sex hormone levels were measured followed by use of real-time PCR to examine selected gene expression in hypothalamic-pituitary-gonadal (HPG) axis and liver. Exposure to 6:2 FTOH significantly increased plasma estradiol (E2) and testosterone (T) levels in both males and females. Furthermore, the ratio of T/E2 was reduced in females while increased in males. In females, the increase of E2 was accompanied by up-regulated hepatic estrogenic receptor alpha (ER alpha) and vitellogenin (VTG1 and VTG3) expression. In males, the elevation of the T level is consistent with the up-regulation of cytochrome P450 c17 alpha-hydroxylase, 17, 20-lase (CYP17) and the down-regulation of cytochrome P450 aromatase A (CYP19A). The present study demonstrated that waterborne exposure to 6:2 FTOH alter plasma sex hormone levels and the ratio of T/E2, as well as the transcriptional profiles of some genes in the HPG axis and liver. The results suggested that FTOHs may disturb fish reproduction through endocrine disrupted activity. (C) 2009 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordFluorotelomer Alcohols Hypothalamic-pituitary-gonadal (Hpg) Axis Gene Transcription Sex Hormone Endocrine Disruption
Department[Liu, Chunsheng; Yu, Liqin; Deng, Jun; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Liu, Chunsheng] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Lam, Paul K. S.; Wu, Rudolf S. S.] City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China
Subject AreaMarine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2009.04.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000268412600006
WOS KeywordCARP CYPRINUS-CARPIO ; ESTROGEN-RECEPTOR ; DANIO-RERIO ; PERFLUORINATED CHEMICALS ; VITELLOGENIN INDUCTION ; GOLDFISH PITUITARY ; FATHEAD MINNOW ; PCR ARRAY ; EXPRESSION ; GONADOTROPIN
Funding OrganizationNational Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7646
Collection期刊论文
Corresponding AuthorZhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Liu, Chunsheng,Yu, Liqin,Deng, Jun,et al. Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish[J]. AQUATIC TOXICOLOGY,2009,93(2-3):131-137.
APA Liu, Chunsheng.,Yu, Liqin.,Deng, Jun.,Lam, Paul K. S..,Wu, Rudolf S. S..,...&Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish.AQUATIC TOXICOLOGY,93(2-3),131-137.
MLA Liu, Chunsheng,et al."Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish".AQUATIC TOXICOLOGY 93.2-3(2009):131-137.
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