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学科主题: Reproductive Biology
题名: Histone H2A Has a Novel Variant in Fish Oocytes
作者: Wu, Nan1; Yue, Hua-Mei1; Chen, Bo1; Gui, Jian-Fang1
通讯作者: Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
关键词: early development ; embryo ; FRAP ; histone H2A variant ; oocyte development ; oocyte maturation ; oogenesis ; ovary ; phosphorylation
刊名: BIOLOGY OF REPRODUCTION
发表日期: 2009-08-01
DOI: 10.1095/biolreprod.108.074955
卷: 81, 期:2, 页:275-283
收录类别: SCI
文章类型: Article
部门归属: [Wu, Nan; Yue, Hua-Mei; Chen, Bo; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301]
类目[WOS]: Reproductive Biology
研究领域[WOS]: Reproductive Biology
摘要: Histone variants and their modification have significant roles in many cellular processes. In this study, we identified and characterized the histone H2A variant h2af1o in fish and revealed its oocyte-specific expression pattern during oogenesis and embryogenesis. Moreover, posttranslational modification of H2af1o was observed that results from phosphorylation during oocyte maturation. To understand the binding dynamics of the novel core histone variant H2af1o in nucleosomes, we cloned ubiquitous gibel carp h2afx as a conventional histone control and investigated the dynamic exchange difference in chromatin by fluorescence recovery after photobleaching. H2af1o has significantly higher mobility in nucleosomes than ubiquitous H2afx. Compared with ubiquitous H2afx, H2af1o has a tightly binding C-terminal and a weakly binding N-terminal. These data indicate that fish oocytes have a novel H2A variant that destabilizes nucleosomes by protruding its N-terminal tail and stabilizes core particles by contracting its C-terminal tail. Our findings suggest that H2af1o may have intrinsic ability to modify chromatin properties during fish oogenesis, oocyte maturation, and early cleavage.
英文摘要: Histone variants and their modification have significant roles in many cellular processes. In this study, we identified and characterized the histone H2A variant h2af1o in fish and revealed its oocyte-specific expression pattern during oogenesis and embryogenesis. Moreover, posttranslational modification of H2af1o was observed that results from phosphorylation during oocyte maturation. To understand the binding dynamics of the novel core histone variant H2af1o in nucleosomes, we cloned ubiquitous gibel carp h2afx as a conventional histone control and investigated the dynamic exchange difference in chromatin by fluorescence recovery after photobleaching. H2af1o has significantly higher mobility in nucleosomes than ubiquitous H2afx. Compared with ubiquitous H2afx, H2af1o has a tightly binding C-terminal and a weakly binding N-terminal. These data indicate that fish oocytes have a novel H2A variant that destabilizes nucleosomes by protruding its N-terminal tail and stabilizes core particles by contracting its C-terminal tail. Our findings suggest that H2af1o may have intrinsic ability to modify chromatin properties during fish oogenesis, oocyte maturation, and early cleavage.
关键词[WOS]: CARASSIUS-AURATUS-GIBELIO ; CELL NUCLEAR TRANSFER ; SILVER CRUCIAN CARP ; C1Q FAMILY-MEMBER ; TERMINAL TAIL ; CHROMATIN MODIFICATIONS ; EPIGENETIC REGULATION ; CORTICAL GRANULES ; LINKER HISTONES ; GENE-EXPRESSION
语种: 英语
WOS记录号: WOS:000268277300005
ISSN号: 0006-3363
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7644
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China

Recommended Citation:
Wu, Nan; Yue, Hua-Mei; Chen, Bo; Gui, Jian-Fang.Histone H2A Has a Novel Variant in Fish Oocytes,BIOLOGY OF REPRODUCTION,2009,81(2):275-283
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