| Histone H2A Has a Novel Variant in Fish Oocytes | |
| Wu, Nan; Yue, Hua-Mei; Chen, Bo; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China | |
| 2009-08-01 | |
| Source Publication | BIOLOGY OF REPRODUCTION
 |
| ISSN | 0006-3363 |
| Volume | 81Issue:2Pages:275-283 |
| Abstract | Histone variants and their modification have significant roles in many cellular processes. In this study, we identified and characterized the histone H2A variant h2af1o in fish and revealed its oocyte-specific expression pattern during oogenesis and embryogenesis. Moreover, posttranslational modification of H2af1o was observed that results from phosphorylation during oocyte maturation. To understand the binding dynamics of the novel core histone variant H2af1o in nucleosomes, we cloned ubiquitous gibel carp h2afx as a conventional histone control and investigated the dynamic exchange difference in chromatin by fluorescence recovery after photobleaching. H2af1o has significantly higher mobility in nucleosomes than ubiquitous H2afx. Compared with ubiquitous H2afx, H2af1o has a tightly binding C-terminal and a weakly binding N-terminal. These data indicate that fish oocytes have a novel H2A variant that destabilizes nucleosomes by protruding its N-terminal tail and stabilizes core particles by contracting its C-terminal tail. Our findings suggest that H2af1o may have intrinsic ability to modify chromatin properties during fish oogenesis, oocyte maturation, and early cleavage.; Histone variants and their modification have significant roles in many cellular processes. In this study, we identified and characterized the histone H2A variant h2af1o in fish and revealed its oocyte-specific expression pattern during oogenesis and embryogenesis. Moreover, posttranslational modification of H2af1o was observed that results from phosphorylation during oocyte maturation. To understand the binding dynamics of the novel core histone variant H2af1o in nucleosomes, we cloned ubiquitous gibel carp h2afx as a conventional histone control and investigated the dynamic exchange difference in chromatin by fluorescence recovery after photobleaching. H2af1o has significantly higher mobility in nucleosomes than ubiquitous H2afx. Compared with ubiquitous H2afx, H2af1o has a tightly binding C-terminal and a weakly binding N-terminal. These data indicate that fish oocytes have a novel H2A variant that destabilizes nucleosomes by protruding its N-terminal tail and stabilizes core particles by contracting its C-terminal tail. Our findings suggest that H2af1o may have intrinsic ability to modify chromatin properties during fish oogenesis, oocyte maturation, and early cleavage. |
| Subtype | Article |
| Keyword | Early Development Embryo Frap Histone H2a Variant Oocyte Development Oocyte Maturation Oogenesis Ovary Phosphorylation |
| Department | [Wu, Nan; Yue, Hua-Mei; Chen, Bo; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China |
| Subject Area | Reproductive Biology |
| DOI | 10.1095/biolreprod.108.074955 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Reproductive Biology |
| WOS Subject | Reproductive Biology |
| WOS ID | WOS:000268277300005 |
| WOS Keyword | CARASSIUS-AURATUS-GIBELIO ; CELL NUCLEAR TRANSFER ; SILVER CRUCIAN CARP ; C1Q FAMILY-MEMBER ; TERMINAL TAIL ; CHROMATIN MODIFICATIONS ; EPIGENETIC REGULATION ; CORTICAL GRANULES ; LINKER HISTONES ; GENE-EXPRESSION |
| Funding Organization | National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7644 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Nan,Yue, Hua-Mei,Chen, Bo,et al. Histone H2A Has a Novel Variant in Fish Oocytes[J]. BIOLOGY OF REPRODUCTION,2009,81(2):275-283. |
| APA | Wu, Nan,Yue, Hua-Mei,Chen, Bo,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China.(2009).Histone H2A Has a Novel Variant in Fish Oocytes.BIOLOGY OF REPRODUCTION,81(2),275-283. |
| MLA | Wu, Nan,et al."Histone H2A Has a Novel Variant in Fish Oocytes".BIOLOGY OF REPRODUCTION 81.2(2009):275-283. |
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