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Histone H2A Has a Novel Variant in Fish Oocytes
Wu, Nan; Yue, Hua-Mei; Chen, Bo; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
2009-08-01
Source PublicationBIOLOGY OF REPRODUCTION
ISSN0006-3363
Volume81Issue:2Pages:275-283
AbstractHistone variants and their modification have significant roles in many cellular processes. In this study, we identified and characterized the histone H2A variant h2af1o in fish and revealed its oocyte-specific expression pattern during oogenesis and embryogenesis. Moreover, posttranslational modification of H2af1o was observed that results from phosphorylation during oocyte maturation. To understand the binding dynamics of the novel core histone variant H2af1o in nucleosomes, we cloned ubiquitous gibel carp h2afx as a conventional histone control and investigated the dynamic exchange difference in chromatin by fluorescence recovery after photobleaching. H2af1o has significantly higher mobility in nucleosomes than ubiquitous H2afx. Compared with ubiquitous H2afx, H2af1o has a tightly binding C-terminal and a weakly binding N-terminal. These data indicate that fish oocytes have a novel H2A variant that destabilizes nucleosomes by protruding its N-terminal tail and stabilizes core particles by contracting its C-terminal tail. Our findings suggest that H2af1o may have intrinsic ability to modify chromatin properties during fish oogenesis, oocyte maturation, and early cleavage.; Histone variants and their modification have significant roles in many cellular processes. In this study, we identified and characterized the histone H2A variant h2af1o in fish and revealed its oocyte-specific expression pattern during oogenesis and embryogenesis. Moreover, posttranslational modification of H2af1o was observed that results from phosphorylation during oocyte maturation. To understand the binding dynamics of the novel core histone variant H2af1o in nucleosomes, we cloned ubiquitous gibel carp h2afx as a conventional histone control and investigated the dynamic exchange difference in chromatin by fluorescence recovery after photobleaching. H2af1o has significantly higher mobility in nucleosomes than ubiquitous H2afx. Compared with ubiquitous H2afx, H2af1o has a tightly binding C-terminal and a weakly binding N-terminal. These data indicate that fish oocytes have a novel H2A variant that destabilizes nucleosomes by protruding its N-terminal tail and stabilizes core particles by contracting its C-terminal tail. Our findings suggest that H2af1o may have intrinsic ability to modify chromatin properties during fish oogenesis, oocyte maturation, and early cleavage.
SubtypeArticle
KeywordEarly Development Embryo Frap Histone H2a Variant Oocyte Development Oocyte Maturation Oogenesis Ovary Phosphorylation
Department[Wu, Nan; Yue, Hua-Mei; Chen, Bo; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
Subject AreaReproductive Biology
DOI10.1095/biolreprod.108.074955
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301]
Indexed BySCI
Language英语
WOS Research AreaReproductive Biology
WOS SubjectReproductive Biology
WOS IDWOS:000268277300005
WOS KeywordCARASSIUS-AURATUS-GIBELIO ; CELL NUCLEAR TRANSFER ; SILVER CRUCIAN CARP ; C1Q FAMILY-MEMBER ; TERMINAL TAIL ; CHROMATIN MODIFICATIONS ; EPIGENETIC REGULATION ; CORTICAL GRANULES ; LINKER HISTONES ; GENE-EXPRESSION
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of Institute of Hydrobiology ; Chinese Academy of Sciences [075A011301]
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Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7644
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wu, Nan,Yue, Hua-Mei,Chen, Bo,et al. Histone H2A Has a Novel Variant in Fish Oocytes[J]. BIOLOGY OF REPRODUCTION,2009,81(2):275-283.
APA Wu, Nan,Yue, Hua-Mei,Chen, Bo,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China.(2009).Histone H2A Has a Novel Variant in Fish Oocytes.BIOLOGY OF REPRODUCTION,81(2),275-283.
MLA Wu, Nan,et al."Histone H2A Has a Novel Variant in Fish Oocytes".BIOLOGY OF REPRODUCTION 81.2(2009):275-283.
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