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学科主题: Biochemistry & Molecular Biology; Physiology; Zoology
题名: Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis
作者: Li, Chuang-Ju1, 3; Wei, Qi-Wei1, 3; Zhou, Li2; Cao, Hong4; Zhang, Yan1, 3; Gui, Jian-Fang2
通讯作者: Wei, QW, Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat & Utilizat, Jinzhou 434000, Peoples R China
关键词: Chinese sturgeon ; Evolution ; Gene expression ; Somatostatin
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
发表日期: 2009-09-01
DOI: 10.1016/j.cbpa.2009.05.012
卷: 154, 期:1, 页:127-134
收录类别: SCI
文章类型: Article
部门归属: [Li, Chuang-Ju; Wei, Qi-Wei; Zhang, Yan] Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat & Utilizat, Jinzhou 434000, Peoples R China; [Zhou, Li; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Li, Chuang-Ju; Wei, Qi-Wei; Zhang, Yan] Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China; [Cao, Hong] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Yangtze River Fisheries Research institute ; Chinese Academy of Fisheries and the Open project of State Key Laboratory of Freshwater Ecology and Biotechnology [20081713007]
类目[WOS]: Biochemistry & Molecular Biology ; Physiology ; Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ; Physiology ; Zoology
摘要: Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNA was synthesized from the hypothalamus of Chinese sturgeon, and the full-length cDNAs of two somatostatin (SS) genes were cloned and sequenced. The first cDNA (AsSS1) encodes a 116-amino acid protein that contains the SS14 sequence at its C-terminal extremity. AsSS1 shows high identity to that of human and other vertebrates. The second cDNA (AsSS2) encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]-SS14 at its C-terminal extremity. Both the two SS mRNAs were expressed in brain and pituitary with different mRNA levels. But in peripheral tissues, AsSS2 was more widely distributed than AsSS1. High mRNA levels of AsSS2 were found in liver, kidney and heart, while low mRNA levels of AsSS2 were also detected in ovary. Throughout embryogenesis and early larval development only AsSS2 mRNAs were detected. Furthermore, in the hypothalamus of one to five year-old Chinese sturgeon, AsSS2 but not AsSS1 maintained stable expression. The mRNA distribution suggests that the Chinese sturgeon AsSS2 products play important physiological functions in adult fish as well as in cell growth and organ differentiation in embryo and larva development. (C) 2009 Elsevier Inc. All rights reserved.
英文摘要: Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNA was synthesized from the hypothalamus of Chinese sturgeon, and the full-length cDNAs of two somatostatin (SS) genes were cloned and sequenced. The first cDNA (AsSS1) encodes a 116-amino acid protein that contains the SS(14) sequence at its C-terminal extremity. AsSS1 shows high identity to that of human and other vertebrates. The second cDNA (AsSS2) encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]-SS(14) at its C-terminal extremity. Both the two SS mRNAs were expressed in brain and pituitary with different mRNA levels. But in peripheral tissues, AsSS2 was more widely distributed than AsSS1. High mRNA levels of AsSS2 were found in liver, kidney and heart, while low mRNA levels of AsSS2 were also detected in ovary. Throughout embryogenesis and early larval development only AsSS2 mRNAs were detected. Furthermore, in the hypothalamus of one to five year-old Chinese sturgeon, AsSS2 but not AsSS1 maintained stable expression. The mRNA distribution suggests that the Chinese sturgeon AsSS2 products play important physiological functions in adult fish as well as in cell growth and organ differentiation in embryo and larva development. (C) 2009 Elsevier Inc. All rights reserved.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; MESSENGER-RNAS ; RAINBOW-TROUT ; GROWTH-HORMONE ; DIFFERENTIAL EXPRESSION ; EMBRYONIC-DEVELOPMENT ; POLYGENIC EXPRESSION ; ENDOCRINE PANCREAS ; UROTENSIN-II ; CLONING
语种: 英语
WOS记录号: WOS:000268625600016
ISSN号: 1095-6433
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7638
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat & Utilizat, Jinzhou 434000, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
4.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China

Recommended Citation:
Li, Chuang-Ju; Wei, Qi-Wei; Zhou, Li; Cao, Hong; Zhang, Yan; Gui, Jian-Fang.Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2009,154(1):127-134
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