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学科主题: Biochemistry & Molecular Biology
题名: Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio)
作者: Liu, Jing-Xia1, 2; Zhai, Yan-Hua1; Gui, Jian-Fang1
通讯作者: Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: AFPIV ; Embryogenesis ; Gibel carp
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2009-09-01
DOI: 10.1007/s11033-008-9412-3
卷: 36, 期:7, 页:2011-2018
收录类别: SCI
文章类型: Article
部门归属: [Liu, Jing-Xia; Zhai, Yan-Hua; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, Jing-Xia] Chinese Acad Sci, Inst Hydrobiol, Grp Mol Toxicol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440]
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: As a new type of AFPs, AFPIV has been firstly identified in longhorn sculpin (Myoxocephalus octodecimspinosus), and in recent years, its cDNA and amino acid sequence have been reported, and its pancreatic synthesis has been firstly reported in polar fish. However, its expression patterns during fish embryogenesis have not been elucidated yet. By differential screening, we cloned the CagAFPIV in gibel carp, Carassius auratus gibelio, demonstrated its predominant expression during embryogenesis. RT-PCR detection revealed that CagAFPIV was first transcribed from blastula stage and kept a high level during embryogenesis and declined remarkably in hatched larva. In situ hybridization revealed that CagAFPIV transcripts were firstly distributed over the margin and marginal blastomere in blastula stage embryos, at the early-gastrula stage the positive signals distributed in the marginal cells and the internalization cells, and later restricted to the cells the yolk syncytial layer (YSL) from later gastrula stage to larva stage. Consistently, the CagAFPIV protein also kept a high level during embryogenesis, and the high protein level retained some days after the larva hatched. Our work, for the first time, revealed the dynamic expression and distribution of CagAFPIV during embryogenesis.
英文摘要: As a new type of AFPs, AFPIV has been firstly identified in longhorn sculpin (Myoxocephalus octodecimspinosus), and in recent years, its cDNA and amino acid sequence have been reported, and its pancreatic synthesis has been firstly reported in polar fish. However, its expression patterns during fish embryogenesis have not been elucidated yet. By differential screening, we cloned the CagAFPIV in gibel carp, Carassius auratus gibelio, demonstrated its predominant expression during embryogenesis. RT-PCR detection revealed that CagAFPIV was first transcribed from blastula stage and kept a high level during embryogenesis and declined remarkably in hatched larva. In situ hybridization revealed that CagAFPIV transcripts were firstly distributed over the margin and marginal blastomere in blastula stage embryos, at the early-gastrula stage the positive signals distributed in the marginal cells and the internalization cells, and later restricted to the cells the yolk syncytial layer (YSL) from later gastrula stage to larva stage. Consistently, the CagAFPIV protein also kept a high level during embryogenesis, and the high protein level retained some days after the larva hatched. Our work, for the first time, revealed the dynamic expression and distribution of CagAFPIV during embryogenesis.
关键词[WOS]: I ANTIFREEZE PROTEINS ; SILVER CRUCIAN CARP ; LONGHORN SCULPIN ; MYOXOCEPHALUS-OCTODECIMSPINOSIS ; CARASSIUS-AURATUS ; ZEBRAFISH ; FISH ; SKIN ; POLYPEPTIDES ; MECHANISMS
语种: 英语
WOS记录号: WOS:000268496800048
ISSN号: 0301-4851
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7632
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Grp Mol Toxicol, Wuhan 430072, Peoples R China

Recommended Citation:
Liu, Jing-Xia; Zhai, Yan-Hua; Gui, Jian-Fang.Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio),MOLECULAR BIOLOGY REPORTS,2009,36(7):2011-2018
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