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Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio)
Liu, Jing-Xia1,2; Zhai, Yan-Hua1; Gui, Jian-Fang1; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009-09-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume36Issue:7Pages:2011-2018
AbstractAs a new type of AFPs, AFPIV has been firstly identified in longhorn sculpin (Myoxocephalus octodecimspinosus), and in recent years, its cDNA and amino acid sequence have been reported, and its pancreatic synthesis has been firstly reported in polar fish. However, its expression patterns during fish embryogenesis have not been elucidated yet. By differential screening, we cloned the CagAFPIV in gibel carp, Carassius auratus gibelio, demonstrated its predominant expression during embryogenesis. RT-PCR detection revealed that CagAFPIV was first transcribed from blastula stage and kept a high level during embryogenesis and declined remarkably in hatched larva. In situ hybridization revealed that CagAFPIV transcripts were firstly distributed over the margin and marginal blastomere in blastula stage embryos, at the early-gastrula stage the positive signals distributed in the marginal cells and the internalization cells, and later restricted to the cells the yolk syncytial layer (YSL) from later gastrula stage to larva stage. Consistently, the CagAFPIV protein also kept a high level during embryogenesis, and the high protein level retained some days after the larva hatched. Our work, for the first time, revealed the dynamic expression and distribution of CagAFPIV during embryogenesis.; As a new type of AFPs, AFPIV has been firstly identified in longhorn sculpin (Myoxocephalus octodecimspinosus), and in recent years, its cDNA and amino acid sequence have been reported, and its pancreatic synthesis has been firstly reported in polar fish. However, its expression patterns during fish embryogenesis have not been elucidated yet. By differential screening, we cloned the CagAFPIV in gibel carp, Carassius auratus gibelio, demonstrated its predominant expression during embryogenesis. RT-PCR detection revealed that CagAFPIV was first transcribed from blastula stage and kept a high level during embryogenesis and declined remarkably in hatched larva. In situ hybridization revealed that CagAFPIV transcripts were firstly distributed over the margin and marginal blastomere in blastula stage embryos, at the early-gastrula stage the positive signals distributed in the marginal cells and the internalization cells, and later restricted to the cells the yolk syncytial layer (YSL) from later gastrula stage to larva stage. Consistently, the CagAFPIV protein also kept a high level during embryogenesis, and the high protein level retained some days after the larva hatched. Our work, for the first time, revealed the dynamic expression and distribution of CagAFPIV during embryogenesis.
SubtypeArticle
KeywordAfpiv Embryogenesis Gibel Carp
Department[Liu, Jing-Xia; Zhai, Yan-Hua; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, Jing-Xia] Chinese Acad Sci, Inst Hydrobiol, Grp Mol Toxicol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology
DOI10.1007/s11033-008-9412-3
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000268496800048
WOS KeywordI ANTIFREEZE PROTEINS ; SILVER CRUCIAN CARP ; LONGHORN SCULPIN ; MYOXOCEPHALUS-OCTODECIMSPINOSIS ; CARASSIUS-AURATUS ; ZEBRAFISH ; FISH ; SKIN ; POLYPEPTIDES ; MECHANISMS
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China-Youth Foundation [30700440]
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7632
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Grp Mol Toxicol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jing-Xia,Zhai, Yan-Hua,Gui, Jian-Fang,et al. Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio)[J]. MOLECULAR BIOLOGY REPORTS,2009,36(7):2011-2018.
APA Liu, Jing-Xia,Zhai, Yan-Hua,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio).MOLECULAR BIOLOGY REPORTS,36(7),2011-2018.
MLA Liu, Jing-Xia,et al."Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio)".MOLECULAR BIOLOGY REPORTS 36.7(2009):2011-2018.
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