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Characterization of the porcine differentially expressed PDK4 gene and association with meat quality
Lan, Jing; Lei, Ming-Gang; Zhang, Yi-Bing; Wang, Jian-Hua; Feng, Xiao-Ting; Xu, De-Quan; Gui, Jian-Fang; Xiong, Yuan-Zhu; Lei, MG, Huazhong Agr Univ, Minist Agr, Key Lab Swine Genet & Breeding, Wuhan 430070, Peoples R China
2009
Source PublicationMOLECULAR BIOLOGY REPORTS
Volume36Issue:7Pages:2003-2010
AbstractTo investigate the differential expression of genes in the skeletal muscle between Yorkshire and Chinese indigenous breed Meishan pigs, suppression subtractive hybridization was carried out and many genes were proved to be expressed significantly different in the two breeds. One gene highly expressed in Meishan but lowly expressed in Yorkshire specific library, shared strong homology with human pyruvate dehydrogenase kinase 4 (PDK4). Using semi-quantity and quantity PCR, We confirmed its differential expression between the two breeds. Temporal and spatial expression analysis indicated that porcine PDK4 gene is highly expressed in skeletal muscle and the highest in neonatal pigs. Complete cDNA cloning and sequence analysis revealed that porcine PDK4 gene contains an open reading frame of 1,221 bp. The deduced amino acid sequence showed conservation in evolution. A G/A mutation in intron 9 was identified and association analysis showed that it was significantly associated with intramuscular fat, muscle water content.
KeywordPorcine Pdk4 Differential Gene Expression Suppression Subtractive Hybridization Meat Quality
Department[Lan, Jing; Lei, Ming-Gang; Wang, Jian-Hua; Feng, Xiao-Ting; Xu, De-Quan; Xiong, Yuan-Zhu] Huazhong Agr Univ, Minist Agr, Key Lab Swine Genet & Breeding, Wuhan 430070, Peoples R China; [Lan, Jing; Lei, Ming-Gang; Wang, Jian-Hua; Feng, Xiao-Ting; Xu, De-Quan; Xiong, Yuan-Zhu] Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China; [Zhang, Yi-Bing; Gui, Jian-Fang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China
Subject AreaBiochemistry & Molecular Biology
Funding OrganizationNational Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6] ; National Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6] ; National Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6] ; National Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6]
Indexed BySCI
Language英语
WOS IDWOS:000268496800047
Funding OrganizationNational Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6] ; National Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6] ; National Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6] ; National Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6]
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Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7630
Collection期刊论文
Corresponding AuthorLei, MG, Huazhong Agr Univ, Minist Agr, Key Lab Swine Genet & Breeding, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Lan, Jing,Lei, Ming-Gang,Zhang, Yi-Bing,et al. Characterization of the porcine differentially expressed PDK4 gene and association with meat quality[J]. MOLECULAR BIOLOGY REPORTS,2009,36(7):2003-2010.
APA Lan, Jing.,Lei, Ming-Gang.,Zhang, Yi-Bing.,Wang, Jian-Hua.,Feng, Xiao-Ting.,...&Lei, MG, Huazhong Agr Univ, Minist Agr, Key Lab Swine Genet & Breeding, Wuhan 430070, Peoples R China.(2009).Characterization of the porcine differentially expressed PDK4 gene and association with meat quality.MOLECULAR BIOLOGY REPORTS,36(7),2003-2010.
MLA Lan, Jing,et al."Characterization of the porcine differentially expressed PDK4 gene and association with meat quality".MOLECULAR BIOLOGY REPORTS 36.7(2009):2003-2010.
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