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Effect of beta-glucan on activity of antioxidant enzymes and Mx gene expression in virus infected grass carp
Kim, Yu-sin; Ke, Fei; Zhang, Qi-Ya; Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume27Issue:2Pages:336-340
AbstractThe effects of beta-glucan, an immunostimulatory agent, on the superoxide dismutase (SOD) and catalase (CAT) activities of erythrocytes and Mx gene expression were studied from grass carp that were challenged with grass carp hemorrhage virus (GCHV). The SOD and CAT activities in erythrocytes and Mx gene expression in spleen from the fish were detected by spectrophotometry and RT-PCR, respectively. Negative control fish were injected with PBS; positive control groups were injected with either P-glucan or GCHV only; and the experimental groups were pre-injected with beta-glucan 15 days prior to injection with GCHV. The results show that the SOD and CAT activities were higher in fish injected with beta-glucan for 15 days than the negative control group injected with PBS. The SOD and CATactivities significantly decreased when the fish were challenged with GCHV, but it was higher in the group pre-treated with beta-glucan than in infected fish not pre-treated, 15 days after GCHV infection. Mx gene expression levels increased during the early stages (at 12 h and 36 h) of GCHV infection, and it remained at higher levels from the 6th till the 10th day in the beta-glucan pre-treated group, but it was failing from the 6th day in the beta-glucan untreated group. The GCHV-infected group pre-treated with P-glucan had a higher survival rate (60%) than the group not pre-treated with P-glucan (20%), suggesting that beta-glucan possesses or enhances anti-viral responses. (C) 2009 Elsevier Ltd. All rights reserved.
KeywordImmunostimulation Beta-glucan Grass Carp Hemorrhage Virus (Gchv) Superoxide Dismutase (Sod) Catalase (Cat) Mx Gene
Department[Kim, Yu-sin; Ke, Fei; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Major Basic Research Program [2009CB118704]; Agriculture Commonweal Scientific Research Plan [200803013]; National Natural Science Foundation of China [30871938]; state key laboratory foundation [2008FBZ16, 095c0223F01] ; National Major Basic Research Program [2009CB118704]; Agriculture Commonweal Scientific Research Plan [200803013]; National Natural Science Foundation of China [30871938]; state key laboratory foundation [2008FBZ16, 095c0223F01] ; National Major Basic Research Program [2009CB118704]; Agriculture Commonweal Scientific Research Plan [200803013]; National Natural Science Foundation of China [30871938]; state key laboratory foundation [2008FBZ16, 095c0223F01] ; National Major Basic Research Program [2009CB118704]; Agriculture Commonweal Scientific Research Plan [200803013]; National Natural Science Foundation of China [30871938]; state key laboratory foundation [2008FBZ16, 095c0223F01]
Indexed BySCI
Language英语
WOS IDWOS:000268914600031
Funding OrganizationNational Major Basic Research Program [2009CB118704]; Agriculture Commonweal Scientific Research Plan [200803013]; National Natural Science Foundation of China [30871938]; state key laboratory foundation [2008FBZ16, 095c0223F01] ; National Major Basic Research Program [2009CB118704]; Agriculture Commonweal Scientific Research Plan [200803013]; National Natural Science Foundation of China [30871938]; state key laboratory foundation [2008FBZ16, 095c0223F01] ; National Major Basic Research Program [2009CB118704]; Agriculture Commonweal Scientific Research Plan [200803013]; National Natural Science Foundation of China [30871938]; state key laboratory foundation [2008FBZ16, 095c0223F01] ; National Major Basic Research Program [2009CB118704]; Agriculture Commonweal Scientific Research Plan [200803013]; National Natural Science Foundation of China [30871938]; state key laboratory foundation [2008FBZ16, 095c0223F01]
Citation statistics
Cited Times:56[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7622
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Kim, Yu-sin,Ke, Fei,Zhang, Qi-Ya,et al. Effect of beta-glucan on activity of antioxidant enzymes and Mx gene expression in virus infected grass carp[J]. FISH & SHELLFISH IMMUNOLOGY,2009,27(2):336-340.
APA Kim, Yu-sin,Ke, Fei,Zhang, Qi-Ya,&Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Effect of beta-glucan on activity of antioxidant enzymes and Mx gene expression in virus infected grass carp.FISH & SHELLFISH IMMUNOLOGY,27(2),336-340.
MLA Kim, Yu-sin,et al."Effect of beta-glucan on activity of antioxidant enzymes and Mx gene expression in virus infected grass carp".FISH & SHELLFISH IMMUNOLOGY 27.2(2009):336-340.
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