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Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR
Huang, Wenmin1,2; Xing, Wei3; Li, Dunhai1; Liu, Yongding1; Li, DH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China
2009-08-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume76Issue:7Pages:1006-1012
AbstractTobacco BY-2 cells were exposed to microcystin-RR (MC-RR) at two concentrations, 60 mu g mL(-1) and 120 mu g mL(-1), to study the changes in morphology and ultrastructure of cells as a result of the exposure. Exposure to the lower concentration for 5 d led to typical apoptotic morphological changes including condensation of nuclear chromatin, creation of a characteristic 'half moon' structure, and cytoplasm shrinkage and decreased cell volume, as revealed through light microscopy. fluorescence microscopy, and transmission electron microscopy, respectively. Exposure to the higher concentration, on the other hand, led to morphological and ultrastructural changes typical of necrosis, such as rupture of the plasma membrane and the nuclear membrane and a marked swelling of cells. The presence of many vacuoles containing unusual deposits points to the involvement of vacuoles in detoxifying MC-RR. Results of the present study indicate that exposure of tobacco BY-2 cells to MC-RR at a lower concentration (60 mu g mL(-1)) results in apoptosis and that to a higher concentration (120 mu g mL(-1)), in necrosis. (C) 2009 Elsevier Ltd. All rights reserved.; Tobacco BY-2 cells were exposed to microcystin-RR (MC-RR) at two concentrations, 60 mu g mL(-1) and 120 mu g mL(-1), to study the changes in morphology and ultrastructure of cells as a result of the exposure. Exposure to the lower concentration for 5 d led to typical apoptotic morphological changes including condensation of nuclear chromatin, creation of a characteristic 'half moon' structure, and cytoplasm shrinkage and decreased cell volume, as revealed through light microscopy. fluorescence microscopy, and transmission electron microscopy, respectively. Exposure to the higher concentration, on the other hand, led to morphological and ultrastructural changes typical of necrosis, such as rupture of the plasma membrane and the nuclear membrane and a marked swelling of cells. The presence of many vacuoles containing unusual deposits points to the involvement of vacuoles in detoxifying MC-RR. Results of the present study indicate that exposure of tobacco BY-2 cells to MC-RR at a lower concentration (60 mu g mL(-1)) results in apoptosis and that to a higher concentration (120 mu g mL(-1)), in necrosis. (C) 2009 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMicrocystin-rr Tobacco By-2 Cells Morphology Ultrastructure Apoptosis Necrosis
Department[Huang, Wenmin; Li, Dunhai; Liu, Yongding] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China; [Huang, Wenmin] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Xing, Wei] Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, PR, Peoples R China
Subject AreaEnvironmental Sciences
DOI10.1016/j.chemosphere.2009.03.074
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10] ; National Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10] ; National Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10] ; National Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000269297200020
WOS KeywordMITOCHONDRIAL PERMEABILITY TRANSITION ; TROUT ONCORHYNCHUS-MYKISS ; ALBA L. SEEDLINGS ; CYANOBACTERIAL TOXIN ; INDUCED APOPTOSIS ; OXIDATIVE STRESS ; SECONDARY METABOLITES ; SUSPENSION CELLS ; RAT HEPATOCYTES ; AQUATIC PLANTS
Funding OrganizationNational Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10] ; National Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10] ; National Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10] ; National Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10]
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7604
Collection期刊论文
Corresponding AuthorLi, DH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, PR, Peoples R China
Recommended Citation
GB/T 7714
Huang, Wenmin,Xing, Wei,Li, Dunhai,et al. Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR[J]. CHEMOSPHERE,2009,76(7):1006-1012.
APA Huang, Wenmin,Xing, Wei,Li, Dunhai,Liu, Yongding,&Li, DH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China.(2009).Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR.CHEMOSPHERE,76(7),1006-1012.
MLA Huang, Wenmin,et al."Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR".CHEMOSPHERE 76.7(2009):1006-1012.
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