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学科主题: Environmental Sciences
题名: Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR
作者: Huang, Wenmin1, 2; Xing, Wei3; Li, Dunhai1; Liu, Yongding1
通讯作者: Li, DH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China
关键词: Microcystin-RR ; Tobacco BY-2 cells ; Morphology ; Ultrastructure ; Apoptosis ; Necrosis
刊名: CHEMOSPHERE
发表日期: 2009-08-01
DOI: 10.1016/j.chemosphere.2009.03.074
卷: 76, 期:7, 页:1006-1012
收录类别: SCI
文章类型: Article
部门归属: [Huang, Wenmin; Li, Dunhai; Liu, Yongding] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China; [Huang, Wenmin] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Xing, Wei] Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, PR, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Programs of China [2002CB412300, 2003CB716801, 2008CR418000]; National Hi-Tech Project [2005AA601010]; Project of Chinese Academy of Sciences [KSCX2-1-10]
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
摘要: Tobacco BY-2 cells were exposed to microcystin-RR (MC-RR) at two concentrations, 60 mu g mL(-1) and 120 mu g mL(-1), to study the changes in morphology and ultrastructure of cells as a result of the exposure. Exposure to the lower concentration for 5 d led to typical apoptotic morphological changes including condensation of nuclear chromatin, creation of a characteristic 'half moon' structure, and cytoplasm shrinkage and decreased cell volume, as revealed through light microscopy. fluorescence microscopy, and transmission electron microscopy, respectively. Exposure to the higher concentration, on the other hand, led to morphological and ultrastructural changes typical of necrosis, such as rupture of the plasma membrane and the nuclear membrane and a marked swelling of cells. The presence of many vacuoles containing unusual deposits points to the involvement of vacuoles in detoxifying MC-RR. Results of the present study indicate that exposure of tobacco BY-2 cells to MC-RR at a lower concentration (60 mu g mL(-1)) results in apoptosis and that to a higher concentration (120 mu g mL(-1)), in necrosis. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要: Tobacco BY-2 cells were exposed to microcystin-RR (MC-RR) at two concentrations, 60 mu g mL(-1) and 120 mu g mL(-1), to study the changes in morphology and ultrastructure of cells as a result of the exposure. Exposure to the lower concentration for 5 d led to typical apoptotic morphological changes including condensation of nuclear chromatin, creation of a characteristic 'half moon' structure, and cytoplasm shrinkage and decreased cell volume, as revealed through light microscopy. fluorescence microscopy, and transmission electron microscopy, respectively. Exposure to the higher concentration, on the other hand, led to morphological and ultrastructural changes typical of necrosis, such as rupture of the plasma membrane and the nuclear membrane and a marked swelling of cells. The presence of many vacuoles containing unusual deposits points to the involvement of vacuoles in detoxifying MC-RR. Results of the present study indicate that exposure of tobacco BY-2 cells to MC-RR at a lower concentration (60 mu g mL(-1)) results in apoptosis and that to a higher concentration (120 mu g mL(-1)), in necrosis. (C) 2009 Elsevier Ltd. All rights reserved.
关键词[WOS]: MITOCHONDRIAL PERMEABILITY TRANSITION ; TROUT ONCORHYNCHUS-MYKISS ; ALBA L. SEEDLINGS ; CYANOBACTERIAL TOXIN ; INDUCED APOPTOSIS ; OXIDATIVE STRESS ; SECONDARY METABOLITES ; SUSPENSION CELLS ; RAT HEPATOCYTES ; AQUATIC PLANTS
语种: 英语
WOS记录号: WOS:000269297200020
ISSN号: 0045-6535
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7604
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotech, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, PR, Peoples R China

Recommended Citation:
Huang, Wenmin; Xing, Wei; Li, Dunhai; Liu, Yongding.Morphological and ultrastructural changes in tobacco BY-2 cells exposed to microcystin-RR,CHEMOSPHERE,2009,76(7):1006-1012
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