IHB OpenIR  > 期刊论文
Determination of microcystin-LR and its metabolites in snail (Bellamya aeruginosa), shrimp (Macrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) from Lake Taihu, China
Zhang, Dawen; Xie, Ping; Chen, Jun; Dai, Ming; Qiu, Tong; Liu, Yaqin; Liang, Gaodao; Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
2009-08-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume76Issue:7Pages:974-981
AbstractThis paper describes seasonal changes of microcystin-LR (MC-LR) and its glutathione (MC-LR-GSH) and cysteine conjugates (MC-LR-Cys) in three aquatic animals - snail (Bellamya aeruginosa), shrimp (fMacrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) collected from Lake Taihu, China. MC-LR, MC-LR-GSH, and MC-LR-Cys were determined by liquid chromatography electrospray ionization mass spectrum (LC-ESI-MS). The mean MC-LR concentrations in the hepatopancreas of snail and shrimp and liver of silver carp were 6.61, 0.24, and 0.027 mu g g(-1) dry weight (DW), respectively: while the average MC-LR-Cys concentrations were 0.50, 0.97, and 5.72 mu g g(-1) DW, respectively. MC-LR-GSH was usually not detectable in these samples. The above results suggest that: (1) in aquatic animals, especially fish, the main excretion form of MC-LR could be MC-LR-Cys, but not MC-LR-GSH, whereas MC-LR-Cys might play an important role in detoxication of MC-LR and (2) that efficiency of MC-LR-Cys formation differs among species. The main detoxication pathway of MC-LR in aquatic animals is suggested as follows: when MC-LR enters into liver/hepatopancreas, it firstly conjugates with polypeptide or protein (including GSH, PP-1 and 2A) containing Cys residues, perhaps also some free cysteine; subsequently, MC-LR-Cys is degraded from these polypeptide or protein; and finally is excreted from animals by the compound of MC-LR-Cys. (C) 2009 Elsevier Ltd. All rights reserved.; This paper describes seasonal changes of microcystin-LR (MC-LR) and its glutathione (MC-LR-GSH) and cysteine conjugates (MC-LR-Cys) in three aquatic animals - snail (Bellamya aeruginosa), shrimp (fMacrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) collected from Lake Taihu, China. MC-LR, MC-LR-GSH, and MC-LR-Cys were determined by liquid chromatography electrospray ionization mass spectrum (LC-ESI-MS). The mean MC-LR concentrations in the hepatopancreas of snail and shrimp and liver of silver carp were 6.61, 0.24, and 0.027 mu g g(-1) dry weight (DW), respectively: while the average MC-LR-Cys concentrations were 0.50, 0.97, and 5.72 mu g g(-1) DW, respectively. MC-LR-GSH was usually not detectable in these samples. The above results suggest that: (1) in aquatic animals, especially fish, the main excretion form of MC-LR could be MC-LR-Cys, but not MC-LR-GSH, whereas MC-LR-Cys might play an important role in detoxication of MC-LR and (2) that efficiency of MC-LR-Cys formation differs among species. The main detoxication pathway of MC-LR in aquatic animals is suggested as follows: when MC-LR enters into liver/hepatopancreas, it firstly conjugates with polypeptide or protein (including GSH, PP-1 and 2A) containing Cys residues, perhaps also some free cysteine; subsequently, MC-LR-Cys is degraded from these polypeptide or protein; and finally is excreted from animals by the compound of MC-LR-Cys. (C) 2009 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMc-lr-gsh Mc-lr-cys Snail Shrimp Silver Carp
Department[Zhang, Dawen; Xie, Ping; Chen, Jun; Dai, Ming; Qiu, Tong; Liu, Yaqin; Liang, Gaodao] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Subject AreaEnvironmental Sciences
DOI10.1016/j.chemosphere.2009.04.034
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationChinese Academy of Sciences [KZCX1-YW-14-6-1]; NSFC-RSE Research Program [30710480] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; NSFC-RSE Research Program [30710480] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; NSFC-RSE Research Program [30710480] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; NSFC-RSE Research Program [30710480]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000269297200016
WOS KeywordIN-VIVO EVIDENCE ; HEPATOTOXIC MICROCYSTINS ; PROTEIN PHOSPHATASE-1 ; GLUTATHIONE CONJUGATE ; PEPTIDE TOXIN ; WATER ; TOXICITY ; BIOACCUMULATION ; IDENTIFICATION ; CYANOBACTERIA
Funding OrganizationChinese Academy of Sciences [KZCX1-YW-14-6-1]; NSFC-RSE Research Program [30710480] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; NSFC-RSE Research Program [30710480] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; NSFC-RSE Research Program [30710480] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; NSFC-RSE Research Program [30710480]
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7602
Collection期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Dawen,Xie, Ping,Chen, Jun,et al. Determination of microcystin-LR and its metabolites in snail (Bellamya aeruginosa), shrimp (Macrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) from Lake Taihu, China[J]. CHEMOSPHERE,2009,76(7):974-981.
APA Zhang, Dawen.,Xie, Ping.,Chen, Jun.,Dai, Ming.,Qiu, Tong.,...&Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.(2009).Determination of microcystin-LR and its metabolites in snail (Bellamya aeruginosa), shrimp (Macrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) from Lake Taihu, China.CHEMOSPHERE,76(7),974-981.
MLA Zhang, Dawen,et al."Determination of microcystin-LR and its metabolites in snail (Bellamya aeruginosa), shrimp (Macrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) from Lake Taihu, China".CHEMOSPHERE 76.7(2009):974-981.
Files in This Item:
File Name/Size DocType Version Access License
Determination of mic(357KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Dawen]'s Articles
[Xie, Ping]'s Articles
[Chen, Jun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Dawen]'s Articles
[Xie, Ping]'s Articles
[Chen, Jun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Dawen]'s Articles
[Xie, Ping]'s Articles
[Chen, Jun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Determination of microcystin-LR and its metabolites in snail (Bellamya aeruginosa), shrimp (Macrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) from Lake Taihu, China.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.