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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)
作者: Su, Jianguo1; Jang, Songhun2; Yang, Chunrong1; Wang, Yaping2; Zhu, Zuoyan2
通讯作者: Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
关键词: Ctenopharyngodon idella ; Toll-like receptor 3 ; Genomic organization ; Gene cloning ; Grass carp reovirus
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2009-09-01
DOI: 10.1016/j.fsi.2009.06.009
卷: 27, 期:3, 页:433-439
收录类别: SCI
文章类型: Article
部门归属: [Su, Jianguo; Yang, Chunrong] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China; [Jang, Songhun; Wang, Yaping; Zhu, Zuoyan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: Toll-like receptor 3 (TLR3) participates in the innate immune response by recognizing viral pathogens. To investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P < 0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要: Toll-like receptor 3 (TLR3) participates in the innate immune response by recognizing viral pathogens. To investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P < 0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases. (C) 2009 Elsevier Ltd. All rights reserved.
关键词[WOS]: TOLL-LIKE RECEPTOR-3 ; DOUBLE-STRANDED-RNA ; VIRAL-INFECTION ; DNA-SEQUENCES ; ACTIVATION ; TLR3 ; ZEBRAFISH ; IMMUNITY ; GENE
语种: 英语
WOS记录号: WOS:000269771000006
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7594
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Su, Jianguo; Jang, Songhun; Yang, Chunrong; Wang, Yaping; Zhu, Zuoyan.Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella),FISH & SHELLFISH IMMUNOLOGY,2009,27(3):433-439
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