IHB OpenIR  > 期刊论文
Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)
Su, Jianguo1; Jang, Songhun2; Yang, Chunrong1; Wang, Yaping2; Zhu, Zuoyan2; Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
2009-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume27Issue:3Pages:433-439
AbstractToll-like receptor 3 (TLR3) participates in the innate immune response by recognizing viral pathogens. To investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P < 0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases. (C) 2009 Elsevier Ltd. All rights reserved.; Toll-like receptor 3 (TLR3) participates in the innate immune response by recognizing viral pathogens. To investigate grass carp immune system responding to GCRV (grass carp reovirus) infection, the full-length cDNA sequence and genomic organization of grass carp TLR3 (CiTLR3) was identified and characterized. The full-length genome sequence of CiTLR3 is composed of 5668 nucleotides, including five exons and four introns. The full-length of CiTLR3 cDNA is 3681 bp in length and encodes a polypeptide of 904 amino acids with an estimated molecular mass of 102,765 Da and a predicted isoelectric point of 8.35. Analysis of the deduced amino acid sequence indicated that CiTLR3 has four main structural domains, including a signal peptide sequence, 14 LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/interleukin-1 receptor) domain. It is most similar to the crucian carp (Carassius auratus) TLR3 amino acid sequence with an identity of 99%. Quantitative RT-PCR analysis showed that CiTLR3 transcripts were significantly up-regulated starting at day 1 and continued through day 7 following GCRV infection (P < 0.05). These data implied that CiTLR3 is involved in antiviral defense, provide molecular and functional information for grass carp TLR3, and implicate their role in mediating immune protection against grass carp viral diseases. (C) 2009 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordCtenopharyngodon Idella Toll-like Receptor 3 Genomic Organization Gene Cloning Grass Carp Reovirus
Department[Su, Jianguo; Yang, Chunrong] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China; [Jang, Songhun; Wang, Yaping; Zhu, Zuoyan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2009.06.009
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508] ; National Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508] ; National Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508] ; National Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000269771000006
WOS KeywordTOLL-LIKE RECEPTOR-3 ; DOUBLE-STRANDED-RNA ; VIRAL-INFECTION ; DNA-SEQUENCES ; ACTIVATION ; TLR3 ; ZEBRAFISH ; IMMUNITY ; GENE
Funding OrganizationNational Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508] ; National Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508] ; National Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508] ; National Natural Science Foundation of China [30871917]; Program for New Century Excellent Talents in University [NCET-08-0466]; SRF for ROCS, SEM [14110104]; Northwest A & F University in China [08080113, 01140309, 01140508]
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7594
Collection期刊论文
Corresponding AuthorSu, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
Affiliation1.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Su, Jianguo,Jang, Songhun,Yang, Chunrong,et al. Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2009,27(3):433-439.
APA Su, Jianguo,Jang, Songhun,Yang, Chunrong,Wang, Yaping,Zhu, Zuoyan,&Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China.(2009).Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,27(3),433-439.
MLA Su, Jianguo,et al."Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 27.3(2009):433-439.
Files in This Item:
File Name/Size DocType Version Access License
Genomic organization(710KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Su, Jianguo]'s Articles
[Jang, Songhun]'s Articles
[Yang, Chunrong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Su, Jianguo]'s Articles
[Jang, Songhun]'s Articles
[Yang, Chunrong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Su, Jianguo]'s Articles
[Jang, Songhun]'s Articles
[Yang, Chunrong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Genomic organization and expression analysis of Toll-like receptor 3 in grass carp (Ctenopharyngodon idella).pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.