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Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus
Zhu, Rong; Zhang, Yibing; Zhang, Qiya; Gui, Jianfang; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009-10-10
Source PublicationPROGRESS IN NATURAL SCIENCE
ISSN1002-0071
Volume19Issue:10Pages:1227-1234
AbstractThe double-stranded RNA (dsRNA)-dependent protein kinase (PKR) belongs to the eIF2 alpha kinase family and plays a critical role in interferon (IFN)-mediated antiviral response. Recently, in Japanese flounder (Paralichthys olivaceus), a PKR gene has been identified. In this study, we showed that PoPKR localized to the cytoplasm, and the dsRNA-binding motifs (dsRBMs) played a determinative role in protein localization. In cultured FEC cells, PoPKR was detected at a low level of constitutive expression but was highly induced after treatment with UV-inactivated grass carp hemorrhagic virus, active SMRV and Poly I:C although with different expression kinetics. In flounder, PoPKR was ubiquitously distributed in all tested tissues, and SMRV infection resulted in significant upregulation at mRNA and protein levels. In order to reveal the role of PoPKR in host antiviral response, its expression upon exposure to various inducers was characterized and further compared with that of PoHRI, which is another eIF2 alpha kinase of flounder. Interestingly, expression comparison revealed that all inducers stimulated upregulation of PoHRI in cultured flounder embryonic cells and fish, with a similar kinetics to PoPKR but to a less extent. These results suggest that, during antiviral immune response, both flounder eIF2 alpha kinases might play similar roles and that PoPKR is the predominant kinase. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.; The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) belongs to the eIF2 alpha kinase family and plays a critical role in interferon (IFN)-mediated antiviral response. Recently, in Japanese flounder (Paralichthys olivaceus), a PKR gene has been identified. In this study, we showed that PoPKR localized to the cytoplasm, and the dsRNA-binding motifs (dsRBMs) played a determinative role in protein localization. In cultured FEC cells, PoPKR was detected at a low level of constitutive expression but was highly induced after treatment with UV-inactivated grass carp hemorrhagic virus, active SMRV and Poly I:C although with different expression kinetics. In flounder, PoPKR was ubiquitously distributed in all tested tissues, and SMRV infection resulted in significant upregulation at mRNA and protein levels. In order to reveal the role of PoPKR in host antiviral response, its expression upon exposure to various inducers was characterized and further compared with that of PoHRI, which is another eIF2 alpha kinase of flounder. Interestingly, expression comparison revealed that all inducers stimulated upregulation of PoHRI in cultured flounder embryonic cells and fish, with a similar kinetics to PoPKR but to a less extent. These results suggest that, during antiviral immune response, both flounder eIF2 alpha kinases might play similar roles and that PoPKR is the predominant kinase. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
SubtypeArticle
KeywordDouble-stranded Rna (dsRna)-dependent Protein Kinase (Pkr) Inductive Expression Paralichthys Olivaceus
Department[Zhu, Rong; Zhang, Yibing; Zhang, Qiya; Gui, Jianfang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
DOI10.1016/j.pnsc.2009.01.013
WOS HeadingsScience & Technology ; Technology
Funding OrganizationNational Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854] ; National Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854] ; National Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854] ; National Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854]
Indexed BySCI
Language英语
WOS Research AreaMaterials Science ; Science & Technology - Other Topics
WOS SubjectMaterials Science, Multidisciplinary ; Multidisciplinary Sciences
WOS IDWOS:000270021300008
WOS KeywordDOUBLE-STRANDED-RNA ; FACTOR 2-ALPHA KINASE ; ENDOPLASMIC-RETICULUM STRESS ; VESICULAR STOMATITIS-VIRUS ; MOLECULAR-CLONING ; BINDING DOMAINS ; EUKARYOTIC INITIATION-FACTOR-2-ALPHA ; FUNCTIONAL-CHARACTERIZATION ; TRANSLATIONAL CONTROL ; VIRAL-INFECTION
Funding OrganizationNational Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854] ; National Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854] ; National Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854] ; National Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7572
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Rong,Zhang, Yibing,Zhang, Qiya,et al. Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus[J]. PROGRESS IN NATURAL SCIENCE,2009,19(10):1227-1234.
APA Zhu, Rong,Zhang, Yibing,Zhang, Qiya,Gui, Jianfang,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus.PROGRESS IN NATURAL SCIENCE,19(10),1227-1234.
MLA Zhu, Rong,et al."Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus".PROGRESS IN NATURAL SCIENCE 19.10(2009):1227-1234.
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