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学科主题: Multidisciplinary Sciences
题名: Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus
作者: Zhu, Rong1; Zhang, Yibing1; Zhang, Qiya1; Gui, Jianfang1
通讯作者: Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Double-stranded RNA (dsRNA)-dependent protein kinase (PKR) ; Inductive expression ; Paralichthys olivaceus
刊名: PROGRESS IN NATURAL SCIENCE
发表日期: 2009-10-10
DOI: 10.1016/j.pnsc.2009.01.013
卷: 19, 期:10, 页:1227-1234
收录类别: SCI
文章类型: Article
部门归属: [Zhu, Rong; Zhang, Yibing; Zhang, Qiya; Gui, Jianfang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Technology
资助者: National Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854]
类目[WOS]: Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]: Materials Science ; Science & Technology - Other Topics
摘要: The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) belongs to the eIF2 alpha kinase family and plays a critical role in interferon (IFN)-mediated antiviral response. Recently, in Japanese flounder (Paralichthys olivaceus), a PKR gene has been identified. In this study, we showed that PoPKR localized to the cytoplasm, and the dsRNA-binding motifs (dsRBMs) played a determinative role in protein localization. In cultured FEC cells, PoPKR was detected at a low level of constitutive expression but was highly induced after treatment with UV-inactivated grass carp hemorrhagic virus, active SMRV and Poly I:C although with different expression kinetics. In flounder, PoPKR was ubiquitously distributed in all tested tissues, and SMRV infection resulted in significant upregulation at mRNA and protein levels. In order to reveal the role of PoPKR in host antiviral response, its expression upon exposure to various inducers was characterized and further compared with that of PoHRI, which is another eIF2 alpha kinase of flounder. Interestingly, expression comparison revealed that all inducers stimulated upregulation of PoHRI in cultured flounder embryonic cells and fish, with a similar kinetics to PoPKR but to a less extent. These results suggest that, during antiviral immune response, both flounder eIF2 alpha kinases might play similar roles and that PoPKR is the predominant kinase. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要: The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) belongs to the eIF2 alpha kinase family and plays a critical role in interferon (IFN)-mediated antiviral response. Recently, in Japanese flounder (Paralichthys olivaceus), a PKR gene has been identified. In this study, we showed that PoPKR localized to the cytoplasm, and the dsRNA-binding motifs (dsRBMs) played a determinative role in protein localization. In cultured FEC cells, PoPKR was detected at a low level of constitutive expression but was highly induced after treatment with UV-inactivated grass carp hemorrhagic virus, active SMRV and Poly I:C although with different expression kinetics. In flounder, PoPKR was ubiquitously distributed in all tested tissues, and SMRV infection resulted in significant upregulation at mRNA and protein levels. In order to reveal the role of PoPKR in host antiviral response, its expression upon exposure to various inducers was characterized and further compared with that of PoHRI, which is another eIF2 alpha kinase of flounder. Interestingly, expression comparison revealed that all inducers stimulated upregulation of PoHRI in cultured flounder embryonic cells and fish, with a similar kinetics to PoPKR but to a less extent. These results suggest that, during antiviral immune response, both flounder eIF2 alpha kinases might play similar roles and that PoPKR is the predominant kinase. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
关键词[WOS]: DOUBLE-STRANDED-RNA ; FACTOR 2-ALPHA KINASE ; ENDOPLASMIC-RETICULUM STRESS ; VESICULAR STOMATITIS-VIRUS ; MOLECULAR-CLONING ; BINDING DOMAINS ; EUKARYOTIC INITIATION-FACTOR-2-ALPHA ; FUNCTIONAL-CHARACTERIZATION ; TRANSLATIONAL CONTROL ; VIRAL-INFECTION
语种: 英语
WOS记录号: WOS:000270021300008
ISSN号: 1002-0071
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7572
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhu, Rong; Zhang, Yibing; Zhang, Qiya; Gui, Jianfang.Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus,PROGRESS IN NATURAL SCIENCE,2009,19(10):1227-1234
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